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Effect of N-acetylcysteine on lipopolysaccharide-induced uveitis in rats.
Jpn J Ophthalmol 2007 Jan-Feb; 51(1):14-20JJ

Abstract

PURPOSE

In this study we investigated the in vivo effect of N-acetylcysteine (NAC) on lipopolysaccharide (LPS)-induced uveitis in rats.

METHODS

To induce uveitis, LPS (100 microg) was injected into subcutaneous tissue of Wistar rats (170-190 g). NAC was injected intraperitoneally. Intracameral levels of protein, cells, nitrite, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6 were determined by spectrophotometry, hemocytometry, and enzyme-linked immunosorbent assay. Expression of TNF-alpha, IL-6, endothelial leukocyte adhesion molecule-1 (E-selectin), intercellular adhesion molecule-1 (ICAM-1), and inducible nitric oxide synthase (iNOS) mRNA was examined by real-time polymerase chain reaction.

RESULTS

LPS injection elevated intracameral protein and cells, and the elevation was inhibited by NAC. LPS injection induced expression of TNF-alpha, IL-6, E-selectin, and ICAM-1 mRNA in the iris/ciliary body at 3 h, and iNOS mRNA at 6 h. The LPS-induced elevation of the mRNA levels was inhibited by NAC. NAC inhibited LPS-induced intracameral elevation of TNF-alpha, IL-6, and nitrite.

CONCLUSION

NAC decreased LPS-induced uveitis in vivo by reducing the expression of proinflammatory cytokines and adhesion molecules.

Authors+Show Affiliations

Department of Ophthalmology, School of Medicine, University of Toyama, Toyama, Japan. ophthal@ms.toyama-mpu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17295135

Citation

Zhang, Xue-Yun, et al. "Effect of N-acetylcysteine On Lipopolysaccharide-induced Uveitis in Rats." Japanese Journal of Ophthalmology, vol. 51, no. 1, 2007, pp. 14-20.
Zhang XY, Hayasaka S, Hayasaka Y, et al. Effect of N-acetylcysteine on lipopolysaccharide-induced uveitis in rats. Jpn J Ophthalmol. 2007;51(1):14-20.
Zhang, X. Y., Hayasaka, S., Hayasaka, Y., Cui, H. S., & Chi, Z. L. (2007). Effect of N-acetylcysteine on lipopolysaccharide-induced uveitis in rats. Japanese Journal of Ophthalmology, 51(1), pp. 14-20.
Zhang XY, et al. Effect of N-acetylcysteine On Lipopolysaccharide-induced Uveitis in Rats. Jpn J Ophthalmol. 2007;51(1):14-20. PubMed PMID: 17295135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of N-acetylcysteine on lipopolysaccharide-induced uveitis in rats. AU - Zhang,Xue-Yun, AU - Hayasaka,Seiji, AU - Hayasaka,Yoriko, AU - Cui,Hu-Shan, AU - Chi,Zai-Long, Y1 - 2007/02/09/ PY - 2006/02/16/received PY - 2006/09/12/accepted PY - 2007/2/14/pubmed PY - 2007/4/18/medline PY - 2007/2/14/entrez SP - 14 EP - 20 JF - Japanese journal of ophthalmology JO - Jpn. J. Ophthalmol. VL - 51 IS - 1 N2 - PURPOSE: In this study we investigated the in vivo effect of N-acetylcysteine (NAC) on lipopolysaccharide (LPS)-induced uveitis in rats. METHODS: To induce uveitis, LPS (100 microg) was injected into subcutaneous tissue of Wistar rats (170-190 g). NAC was injected intraperitoneally. Intracameral levels of protein, cells, nitrite, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6 were determined by spectrophotometry, hemocytometry, and enzyme-linked immunosorbent assay. Expression of TNF-alpha, IL-6, endothelial leukocyte adhesion molecule-1 (E-selectin), intercellular adhesion molecule-1 (ICAM-1), and inducible nitric oxide synthase (iNOS) mRNA was examined by real-time polymerase chain reaction. RESULTS: LPS injection elevated intracameral protein and cells, and the elevation was inhibited by NAC. LPS injection induced expression of TNF-alpha, IL-6, E-selectin, and ICAM-1 mRNA in the iris/ciliary body at 3 h, and iNOS mRNA at 6 h. The LPS-induced elevation of the mRNA levels was inhibited by NAC. NAC inhibited LPS-induced intracameral elevation of TNF-alpha, IL-6, and nitrite. CONCLUSION: NAC decreased LPS-induced uveitis in vivo by reducing the expression of proinflammatory cytokines and adhesion molecules. SN - 0021-5155 UR - https://www.unboundmedicine.com/medline/citation/17295135/Effect_of_N_acetylcysteine_on_lipopolysaccharide_induced_uveitis_in_rats_ L2 - https://dx.doi.org/10.1007/s10384-006-0382-5 DB - PRIME DP - Unbound Medicine ER -