TY - JOUR T1 - Liquid chromatography/tandem mass spectrometry method for the simultaneous determination of olanzapine, risperidone, 9-hydroxyrisperidone, clozapine, haloperidol and ziprasidone in rat plasma. AU - Zhang,Guodong, AU - Terry,Alvin V,Jr AU - Bartlett,Michael G, PY - 2007/2/14/pubmed PY - 2007/5/10/medline PY - 2007/2/14/entrez SP - 920 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 6 N2 - A simple, sensitive and rapid liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) method was developed and validated for simultaneous quantification of olanzapine, clozapine, ziprasidone, haloperidol, risperidone, and its active metabolite 9-hydroxyrisperidone, in rat plasma using midazolam as internal standard (IS). The analytes were extracted from rat plasma using a single step liquid-liquid extraction technique. The compounds were separated on a Waters Atlantis dC-18 (30 mm x 2.1 mm i.d., 3 microm) column using a mobile phase of acetonitrile/5 mM ammonium formate (pH 6.1 adjusted with formic acid) with gradient elution. All of the analytes were detected in positive ion mode using multiple reaction monitoring (MRM). The method was validated and the specificity, linearity, lower limit of quantitation (LLOQ), precision, accuracy, recoveries and stability were determined. LLOQ was 0.1 ng/mL and correlation coefficient (R(2)) values for the linear range of 0.1-100 ng/mL were 0.997 or greater for all the analytes. The intra-day and inter-day precision and accuracy were better than 8.05%. The relative and absolute recovery was above 77% and matrix effects were low for all the analytes except for ziprasidone. This validated method has been successfully used to quantify the plasma concentration of the analytes after chronic treatment with antipsychotic drugs. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17295424/Liquid_chromatography/tandem_mass_spectrometry_method_for_the_simultaneous_determination_of_olanzapine_risperidone_9_hydroxyrisperidone_clozapine_haloperidol_and_ziprasidone_in_rat_plasma_ L2 - https://doi.org/10.1002/rcm.2914 DB - PRIME DP - Unbound Medicine ER -