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Double immunostaining with p63 and high-molecular-weight cytokeratins distinguishes borderline papillary lesions of the breast.
Pathol Int. 2007 Mar; 57(3):126-32.PI

Abstract

Papillary breast lesions remain a source of diagnostic confusion because the full range of epithelial proliferations may arise within, or secondarily involve, papilloma. The expression of p63 and high-molecular-weight cytokeratins (HMWCK) was studied simultaneously in 33 papillary lesions including intraductal papilloma (IP, n = 10), atypical papilloma (AP, n = 8) and intraductal papillary carcinoma (IPC, n = 15) by double immunostaining. The myoepithelial cell nuclei were stained dark brown whereas the cytoplasms of usual ductal hyperplasia (UDH) and myoepithelium were stained purple. The myoepithelial layer was recognized as a dark brown dotted line at the epithelial stromal junction in all IP (10/10), most AP (7/8) and some IPC (7/15), suggesting that the retained myoepithelial layer in the papillary processes does not necessarily guarantee benignity. However, the malignant epithelial cells in AP and IPC were typically recognized as monotonous populations unstained with either chromogen. These monotonous cells contrasted with the proliferating cells of UDH in papilloma, which had intense purple cytoplasm in a mosaic-like fashion. The present data suggest that the double immunostaining with the two popular antibodies p63 and HMWCK is a useful tool for reproducible classification of papillary breast lesions.

Authors+Show Affiliations

Department of Pathology, Nagoya Medical Center, 4-1-1 Sannomaru, Naka-ku, Nagoya 460-0001, Japan. shu-kkr@umin.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17295644

Citation

Ichihara, Shu, et al. "Double Immunostaining With P63 and High-molecular-weight Cytokeratins Distinguishes Borderline Papillary Lesions of the Breast." Pathology International, vol. 57, no. 3, 2007, pp. 126-32.
Ichihara S, Fujimoto T, Hashimoto K, et al. Double immunostaining with p63 and high-molecular-weight cytokeratins distinguishes borderline papillary lesions of the breast. Pathol Int. 2007;57(3):126-32.
Ichihara, S., Fujimoto, T., Hashimoto, K., Moritani, S., Hasegawa, M., & Yokoi, T. (2007). Double immunostaining with p63 and high-molecular-weight cytokeratins distinguishes borderline papillary lesions of the breast. Pathology International, 57(3), 126-32.
Ichihara S, et al. Double Immunostaining With P63 and High-molecular-weight Cytokeratins Distinguishes Borderline Papillary Lesions of the Breast. Pathol Int. 2007;57(3):126-32. PubMed PMID: 17295644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Double immunostaining with p63 and high-molecular-weight cytokeratins distinguishes borderline papillary lesions of the breast. AU - Ichihara,Shu, AU - Fujimoto,Takuya, AU - Hashimoto,Katsunori, AU - Moritani,Suzuko, AU - Hasegawa,Masaki, AU - Yokoi,Toyoharu, PY - 2007/2/14/pubmed PY - 2007/4/6/medline PY - 2007/2/14/entrez SP - 126 EP - 32 JF - Pathology international JO - Pathol Int VL - 57 IS - 3 N2 - Papillary breast lesions remain a source of diagnostic confusion because the full range of epithelial proliferations may arise within, or secondarily involve, papilloma. The expression of p63 and high-molecular-weight cytokeratins (HMWCK) was studied simultaneously in 33 papillary lesions including intraductal papilloma (IP, n = 10), atypical papilloma (AP, n = 8) and intraductal papillary carcinoma (IPC, n = 15) by double immunostaining. The myoepithelial cell nuclei were stained dark brown whereas the cytoplasms of usual ductal hyperplasia (UDH) and myoepithelium were stained purple. The myoepithelial layer was recognized as a dark brown dotted line at the epithelial stromal junction in all IP (10/10), most AP (7/8) and some IPC (7/15), suggesting that the retained myoepithelial layer in the papillary processes does not necessarily guarantee benignity. However, the malignant epithelial cells in AP and IPC were typically recognized as monotonous populations unstained with either chromogen. These monotonous cells contrasted with the proliferating cells of UDH in papilloma, which had intense purple cytoplasm in a mosaic-like fashion. The present data suggest that the double immunostaining with the two popular antibodies p63 and HMWCK is a useful tool for reproducible classification of papillary breast lesions. SN - 1320-5463 UR - https://www.unboundmedicine.com/medline/citation/17295644/Double_immunostaining_with_p63_and_high_molecular_weight_cytokeratins_distinguishes_borderline_papillary_lesions_of_the_breast_ L2 - https://doi.org/10.1111/j.1440-1827.2006.02069.x DB - PRIME DP - Unbound Medicine ER -