Tags

Type your tag names separated by a space and hit enter

Poor ovarian response to gonadotropin stimulation is associated with low expression of follicle-stimulating hormone receptor in granulosa cells.
Fertil Steril. 2007 Jun; 87(6):1350-6.FS

Abstract

OBJECTIVE

To explore the importance of follicle-stimulating hormone receptor (FSHR) in granulosa cells in the ovarian response to gonadotropin stimulation.

DESIGN

Prospective study.

SETTING

A women's hospital in China.

PATIENT(S)

One hundred infertile women undergoing ovarian stimulation with recombinant follicle-stimulating hormone (rFSH).

INTERVENTION(S)

These women were divided into three groups: poor, moderate, and high responders, according to the number of follicles with diameter >/=14 mm. The FSHR expression at both mRNA and protein levels was determined by either reverse transcription-polymerase chain reaction or Western blot in granulosa cells. E(2) concentrations in serum and FSH levels in serum/follicular fluid (FF) were measured by electrochemiluminescence immunoassay.

MAIN OUTCOME MEASURE(S)

Relative expression of mRNA and protein of FSHR in granulosa cells, serum E(2), FSH level in serum and FF, and the number of mature follicles.

RESULT(S)

The expression of FSHR, at both the mRNA and protein levels, was significantly different among the three groups, with the lowest expression in the poor responders. The level of FSHR protein was positively correlated with the peak level of serum E(2) and the number of mature oocytes. FSH levels in FF and the dosage of rFSH used were significantly different among the three groups, with the highest values in the poor responders.

CONCLUSION(S)

Different levels of FSHR expression in granulosa cells result in different ovarian response, and lower expression of FSHR may account for poor ovarian response to gonadotropin stimulation, which suggests the critical role of FSHR in the ovarian response to gonadotropin stimulation.

Authors+Show Affiliations

Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17296182

Citation

Cai, Jie, et al. "Poor Ovarian Response to Gonadotropin Stimulation Is Associated With Low Expression of Follicle-stimulating Hormone Receptor in Granulosa Cells." Fertility and Sterility, vol. 87, no. 6, 2007, pp. 1350-6.
Cai J, Lou HY, Dong MY, et al. Poor ovarian response to gonadotropin stimulation is associated with low expression of follicle-stimulating hormone receptor in granulosa cells. Fertil Steril. 2007;87(6):1350-6.
Cai, J., Lou, H. Y., Dong, M. Y., Lu, X. E., Zhu, Y. M., Gao, H. J., & Huang, H. F. (2007). Poor ovarian response to gonadotropin stimulation is associated with low expression of follicle-stimulating hormone receptor in granulosa cells. Fertility and Sterility, 87(6), 1350-6.
Cai J, et al. Poor Ovarian Response to Gonadotropin Stimulation Is Associated With Low Expression of Follicle-stimulating Hormone Receptor in Granulosa Cells. Fertil Steril. 2007;87(6):1350-6. PubMed PMID: 17296182.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poor ovarian response to gonadotropin stimulation is associated with low expression of follicle-stimulating hormone receptor in granulosa cells. AU - Cai,Jie, AU - Lou,Hang-ying, AU - Dong,Min-yue, AU - Lu,Xiu-e, AU - Zhu,Yi-min, AU - Gao,Hui-juan, AU - Huang,He-feng, Y1 - 2007/02/12/ PY - 2006/03/16/received PY - 2006/11/09/revised PY - 2006/11/09/accepted PY - 2007/2/14/pubmed PY - 2007/9/15/medline PY - 2007/2/14/entrez SP - 1350 EP - 6 JF - Fertility and sterility JO - Fertil Steril VL - 87 IS - 6 N2 - OBJECTIVE: To explore the importance of follicle-stimulating hormone receptor (FSHR) in granulosa cells in the ovarian response to gonadotropin stimulation. DESIGN: Prospective study. SETTING: A women's hospital in China. PATIENT(S): One hundred infertile women undergoing ovarian stimulation with recombinant follicle-stimulating hormone (rFSH). INTERVENTION(S): These women were divided into three groups: poor, moderate, and high responders, according to the number of follicles with diameter >/=14 mm. The FSHR expression at both mRNA and protein levels was determined by either reverse transcription-polymerase chain reaction or Western blot in granulosa cells. E(2) concentrations in serum and FSH levels in serum/follicular fluid (FF) were measured by electrochemiluminescence immunoassay. MAIN OUTCOME MEASURE(S): Relative expression of mRNA and protein of FSHR in granulosa cells, serum E(2), FSH level in serum and FF, and the number of mature follicles. RESULT(S): The expression of FSHR, at both the mRNA and protein levels, was significantly different among the three groups, with the lowest expression in the poor responders. The level of FSHR protein was positively correlated with the peak level of serum E(2) and the number of mature oocytes. FSH levels in FF and the dosage of rFSH used were significantly different among the three groups, with the highest values in the poor responders. CONCLUSION(S): Different levels of FSHR expression in granulosa cells result in different ovarian response, and lower expression of FSHR may account for poor ovarian response to gonadotropin stimulation, which suggests the critical role of FSHR in the ovarian response to gonadotropin stimulation. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/17296182/Poor_ovarian_response_to_gonadotropin_stimulation_is_associated_with_low_expression_of_follicle_stimulating_hormone_receptor_in_granulosa_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(06)04497-9 DB - PRIME DP - Unbound Medicine ER -