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Deranged expression of follistatin and follistatin-like protein in women with ovarian endometriosis.
Fertil Steril. 2007 Jul; 88(1):200-5.FS

Abstract

OBJECTIVE

To evaluate the messenger RNA (mRNA) expression and peptide localization of follistatin and follistatin-like protein (FLRG) in ovarian endometriosis, compared to healthy human endometrium.

DESIGN

Samples of ovarian endometriotic and healthy endometrial tissues were processed by semiquantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry.

SETTING

Academic health centers in Siena, Italy, and Belo Horizonte, Brazil.

PATIENT(S)

Women with endometrioma who underwent laparoscopic excision of ovarian endometriotic cysts (n = 16), and healthy, nonpregnant women (n = 18, control group).

MAIN OUTCOME MEASURE(S)

Immunostaining and relative quantification of follistatin and FLRG mRNA in ovarian endometriosis and eutopic endometrium.

RESULT(S)

Both ovarian endometriosis and healthy endometrium expressed and localized follistatin and FLRG. In endometriotic glands, follistatin immunostaining was homogeneously distributed throughout the cytoplasm of the epithelial cells, contrasting with normal eutopic endometrium, where follistatin expression was focal, irregular, and confined to the basal side of the glands. Follistatin-like protein was immunolocalized in the nuclei of both glandular epithelial cells and stromal cells, with less intense staining in endometriotic samples. The relative intensity of follistatin and FLRG immunostaining was significantly higher and lower, respectively, in endometriosis than in controls. The expression of follistatin mRNA was higher, while that of FLRG mRNA was lower, in ovarian endometriosis than in healthy eutopic endometrium.

CONCLUSION(S)

Ovarian endometriotic lesions show a deranged expression of FLRG and follistatin, which are activin A-binding proteins. This may result in an altered effect of activin A on angiogenesis and/or endometrial differentiation.

Authors+Show Affiliations

Section of Obstetrics and Gynecology, Department of Pediatrics, Obstetrics, and Reproductive Medicine, University of Siena, Siena, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17296189

Citation

Torres, Paulo B., et al. "Deranged Expression of Follistatin and Follistatin-like Protein in Women With Ovarian Endometriosis." Fertility and Sterility, vol. 88, no. 1, 2007, pp. 200-5.
Torres PB, Florio P, Ferreira MC, et al. Deranged expression of follistatin and follistatin-like protein in women with ovarian endometriosis. Fertil Steril. 2007;88(1):200-5.
Torres, P. B., Florio, P., Ferreira, M. C., Torricelli, M., Reis, F. M., & Petraglia, F. (2007). Deranged expression of follistatin and follistatin-like protein in women with ovarian endometriosis. Fertility and Sterility, 88(1), 200-5.
Torres PB, et al. Deranged Expression of Follistatin and Follistatin-like Protein in Women With Ovarian Endometriosis. Fertil Steril. 2007;88(1):200-5. PubMed PMID: 17296189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deranged expression of follistatin and follistatin-like protein in women with ovarian endometriosis. AU - Torres,Paulo B, AU - Florio,Pasquale, AU - Ferreira,Marcia C, AU - Torricelli,Michela, AU - Reis,Fernando M, AU - Petraglia,Felice, Y1 - 2007/02/12/ PY - 2006/05/17/received PY - 2006/10/31/revised PY - 2006/11/16/accepted PY - 2007/2/14/pubmed PY - 2007/8/1/medline PY - 2007/2/14/entrez SP - 200 EP - 5 JF - Fertility and sterility JO - Fertil. Steril. VL - 88 IS - 1 N2 - OBJECTIVE: To evaluate the messenger RNA (mRNA) expression and peptide localization of follistatin and follistatin-like protein (FLRG) in ovarian endometriosis, compared to healthy human endometrium. DESIGN: Samples of ovarian endometriotic and healthy endometrial tissues were processed by semiquantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry. SETTING: Academic health centers in Siena, Italy, and Belo Horizonte, Brazil. PATIENT(S): Women with endometrioma who underwent laparoscopic excision of ovarian endometriotic cysts (n = 16), and healthy, nonpregnant women (n = 18, control group). MAIN OUTCOME MEASURE(S): Immunostaining and relative quantification of follistatin and FLRG mRNA in ovarian endometriosis and eutopic endometrium. RESULT(S): Both ovarian endometriosis and healthy endometrium expressed and localized follistatin and FLRG. In endometriotic glands, follistatin immunostaining was homogeneously distributed throughout the cytoplasm of the epithelial cells, contrasting with normal eutopic endometrium, where follistatin expression was focal, irregular, and confined to the basal side of the glands. Follistatin-like protein was immunolocalized in the nuclei of both glandular epithelial cells and stromal cells, with less intense staining in endometriotic samples. The relative intensity of follistatin and FLRG immunostaining was significantly higher and lower, respectively, in endometriosis than in controls. The expression of follistatin mRNA was higher, while that of FLRG mRNA was lower, in ovarian endometriosis than in healthy eutopic endometrium. CONCLUSION(S): Ovarian endometriotic lesions show a deranged expression of FLRG and follistatin, which are activin A-binding proteins. This may result in an altered effect of activin A on angiogenesis and/or endometrial differentiation. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/17296189/Deranged_expression_of_follistatin_and_follistatin_like_protein_in_women_with_ovarian_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(06)04547-X DB - PRIME DP - Unbound Medicine ER -