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Liposomised recombinant ribosomal L7/L12 protein protects BALB/c mice against Brucella abortus 544 infection.
Vaccine. 2007 May 04; 25(18):3692-704.V

Abstract

Brucella abortus, a facultative intracellular pathogen, is of tremendous zoonotic importance because of its ability to induce spontaneous abortion in cattle and other livestock. It is also known to cause persistent undulant fever, endocarditis, arthritis, osteomyelitis and meningitis in humans. The available vaccines against this dreadful infection suffer from limitations like short-term immunity, increased risk of hypersensitivity and low prophylactic index in the recipients. In the present study, we have demonstrated that liposomal form of a recombinant ribosomal L7/L12 protein, a B-T cell antigen of B. abortus, activates strong immune response in the host. In contrast, free antigen generates moderate immune response in the immunised animals. The liposomisation of rL7/L12 protein causes tremendous increase in cell-mediated immune response in terms of delayed type hypersensitivity, T-cell proliferation and up-regulation in type I cytokine expression, etc. Moreover, the liposome encapsulated antigen elicited stronger humoral immune response as compared to standard vaccine (S-19) or IFA-L7/L12 combination in the immunised animals. The effectiveness of liposome-based vaccine was also substantiated by better systemic clearance of bacterial load after challenging the animals with B. abortus 544 pathogen. The results of the present study suggest the potential of liposome-based rL7/L12 antigen as prospective and efficient candidate vaccine capable of eliciting both cell mediated as well as humoral immune responses against experimental murine brucellosis.

Authors+Show Affiliations

Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh-202002, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17296251

Citation

Mallick, A I., et al. "Liposomised Recombinant Ribosomal L7/L12 Protein Protects BALB/c Mice Against Brucella Abortus 544 Infection." Vaccine, vol. 25, no. 18, 2007, pp. 3692-704.
Mallick AI, Singha H, Chaudhuri P, et al. Liposomised recombinant ribosomal L7/L12 protein protects BALB/c mice against Brucella abortus 544 infection. Vaccine. 2007;25(18):3692-704.
Mallick, A. I., Singha, H., Chaudhuri, P., Nadeem, A., Khan, S. A., Dar, K. A., & Owais, M. (2007). Liposomised recombinant ribosomal L7/L12 protein protects BALB/c mice against Brucella abortus 544 infection. Vaccine, 25(18), 3692-704.
Mallick AI, et al. Liposomised Recombinant Ribosomal L7/L12 Protein Protects BALB/c Mice Against Brucella Abortus 544 Infection. Vaccine. 2007 May 4;25(18):3692-704. PubMed PMID: 17296251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liposomised recombinant ribosomal L7/L12 protein protects BALB/c mice against Brucella abortus 544 infection. AU - Mallick,A I, AU - Singha,H, AU - Chaudhuri,P, AU - Nadeem,Ahmad, AU - Khan,Shadab Ahmad, AU - Dar,Khurshid Ahmad, AU - Owais,M, Y1 - 2007/01/25/ PY - 2006/10/10/received PY - 2007/01/08/revised PY - 2007/01/08/accepted PY - 2007/2/14/pubmed PY - 2007/6/30/medline PY - 2007/2/14/entrez SP - 3692 EP - 704 JF - Vaccine JO - Vaccine VL - 25 IS - 18 N2 - Brucella abortus, a facultative intracellular pathogen, is of tremendous zoonotic importance because of its ability to induce spontaneous abortion in cattle and other livestock. It is also known to cause persistent undulant fever, endocarditis, arthritis, osteomyelitis and meningitis in humans. The available vaccines against this dreadful infection suffer from limitations like short-term immunity, increased risk of hypersensitivity and low prophylactic index in the recipients. In the present study, we have demonstrated that liposomal form of a recombinant ribosomal L7/L12 protein, a B-T cell antigen of B. abortus, activates strong immune response in the host. In contrast, free antigen generates moderate immune response in the immunised animals. The liposomisation of rL7/L12 protein causes tremendous increase in cell-mediated immune response in terms of delayed type hypersensitivity, T-cell proliferation and up-regulation in type I cytokine expression, etc. Moreover, the liposome encapsulated antigen elicited stronger humoral immune response as compared to standard vaccine (S-19) or IFA-L7/L12 combination in the immunised animals. The effectiveness of liposome-based vaccine was also substantiated by better systemic clearance of bacterial load after challenging the animals with B. abortus 544 pathogen. The results of the present study suggest the potential of liposome-based rL7/L12 antigen as prospective and efficient candidate vaccine capable of eliciting both cell mediated as well as humoral immune responses against experimental murine brucellosis. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/17296251/Liposomised_recombinant_ribosomal_L7/L12_protein_protects_BALB/c_mice_against_Brucella_abortus_544_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(07)00027-8 DB - PRIME DP - Unbound Medicine ER -