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Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily.
Proc Natl Acad Sci U S A. 1992 Jan 01; 89(1):426-30.PN

Abstract

The majority of human urinary stones are primarily composed of calcium salts. Although normal urine is frequently supersaturated with respect to calcium oxalate, most humans do not form stones. Inhibitors are among the multiple factors that may influence the complex process of urinary stone formation. We have isolated an inhibitor of calcium oxalate crystal growth from human urine by monoclonal antibody immunoaffinity chromatography. The N-terminal amino acid sequence and acidic amino acid content of this aspartic acid-rich protein, uropontin, are similar to those of other pontin proteins from bone, plasma, breast milk, and cells. The inhibitory effect of uropontin on calcium oxalate crystal growth in vitro supports the concept that pontins may have a regulatory role. This function would be analogous to that of other members of the aspartic acid-rich protein superfamily, which stereospecifically regulate the mineralization fronts of calcium-containing crystals.

Links

Publisher Full Text
ncbi.nlm.nih.gov
pnas.org
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Authors+Show Affiliations

Shiraga H
Department of Pediatrics, University of Pennsylvania, Philadelphia.
Min W
No affiliation info available
VanDusen WJ
No affiliation info available
Clayman MD
No affiliation info available
Miner D
No affiliation info available
Terrell CH
No affiliation info available
Sherbotie JR
No affiliation info available
Foreman JW
No affiliation info available
Przysiecki C
No affiliation info available
Neilson EG
No affiliation info available

MeSH

Amino Acid SequenceAntibodies, MonoclonalAspartic AcidCalcium OxalateCrystallizationHumansMolecular Sequence DataMultigene FamilyOsteopontinProteinsSequence AlignmentSialoglycoproteinsUrinary Bladder Calculi

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1729712

Citation

Shiraga, H, et al. "Inhibition of Calcium Oxalate Crystal Growth in Vitro By Uropontin: Another Member of the Aspartic Acid-rich Protein Superfamily." Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 1, 1992, pp. 426-30.
Shiraga H, Min W, VanDusen WJ, et al. Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proc Natl Acad Sci U S A. 1992;89(1):426-30.
Shiraga, H., Min, W., VanDusen, W. J., Clayman, M. D., Miner, D., Terrell, C. H., Sherbotie, J. R., Foreman, J. W., Przysiecki, C., & Neilson, E. G. (1992). Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proceedings of the National Academy of Sciences of the United States of America, 89(1), 426-30.
Shiraga H, et al. Inhibition of Calcium Oxalate Crystal Growth in Vitro By Uropontin: Another Member of the Aspartic Acid-rich Protein Superfamily. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):426-30. PubMed PMID: 1729712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. A1 - Shiraga,H, AU - Min,W, AU - VanDusen,W J, AU - Clayman,M D, AU - Miner,D, AU - Terrell,C H, AU - Sherbotie,J R, AU - Foreman,J W, AU - Przysiecki,C, AU - Neilson,E G, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 426 EP - 30 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 89 IS - 1 N2 - The majority of human urinary stones are primarily composed of calcium salts. Although normal urine is frequently supersaturated with respect to calcium oxalate, most humans do not form stones. Inhibitors are among the multiple factors that may influence the complex process of urinary stone formation. We have isolated an inhibitor of calcium oxalate crystal growth from human urine by monoclonal antibody immunoaffinity chromatography. The N-terminal amino acid sequence and acidic amino acid content of this aspartic acid-rich protein, uropontin, are similar to those of other pontin proteins from bone, plasma, breast milk, and cells. The inhibitory effect of uropontin on calcium oxalate crystal growth in vitro supports the concept that pontins may have a regulatory role. This function would be analogous to that of other members of the aspartic acid-rich protein superfamily, which stereospecifically regulate the mineralization fronts of calcium-containing crystals. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/1729712/Inhibition_of_calcium_oxalate_crystal_growth_in_vitro_by_uropontin:_another_member_of_the_aspartic_acid_rich_protein_superfamily_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1729712 DB - PRIME DP - Unbound Medicine ER -