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Imaging technique for the screening of protein-protein interactions using scattered light under surface plasmon resonance conditions.
Anal Chem. 2007 Feb 15; 79(4):1349-55.AC

Abstract

We propose a novel technique to detect protein-protein interactions in microarray format. The technique involves measuring scattered light under surface plasmon resonance (SPR) conditions. We have shown that the maximum scattering angle correlates with the traditionally employed reflection minimum. Panoramic scanning of scattered light under SPR conditions has all the functional advantages of the SPR technique. In addition, the proposed technique simplifies device design, increases the dynamic range of analysis, and integrates data with those from surface-plasmon field-enhanced fluorescence spectroscopy. We demonstrate the technique by showing direct protein-protein interaction between protein A and either rabbit antibodies or human serum.

Authors+Show Affiliations

Savchenko A
V. Lashkaryov Institute of Semiconductor Physics, National Academy of Sciences, 41 Prospect Nauki, 03028 Kyiv, Ukraine.
Kashuba E
No affiliation info available
Kashuba V
No affiliation info available
Snopok B
No affiliation info available

MeSH

AnimalsBlood ProteinsEquipment DesignEquipment Failure AnalysisImmunoglobulin GLightProtein Array AnalysisProtein BindingProtein Interaction MappingRabbitsScattering, RadiationSensitivity and SpecificityStaphylococcal Protein AStaphylococcus aureusSurface Plasmon Resonance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17297933

Citation

Savchenko, Andrej, et al. "Imaging Technique for the Screening of Protein-protein Interactions Using Scattered Light Under Surface Plasmon Resonance Conditions." Analytical Chemistry, vol. 79, no. 4, 2007, pp. 1349-55.
Savchenko A, Kashuba E, Kashuba V, et al. Imaging technique for the screening of protein-protein interactions using scattered light under surface plasmon resonance conditions. Anal Chem. 2007;79(4):1349-55.
Savchenko, A., Kashuba, E., Kashuba, V., & Snopok, B. (2007). Imaging technique for the screening of protein-protein interactions using scattered light under surface plasmon resonance conditions. Analytical Chemistry, 79(4), 1349-55.
Savchenko A, et al. Imaging Technique for the Screening of Protein-protein Interactions Using Scattered Light Under Surface Plasmon Resonance Conditions. Anal Chem. 2007 Feb 15;79(4):1349-55. PubMed PMID: 17297933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Imaging technique for the screening of protein-protein interactions using scattered light under surface plasmon resonance conditions. AU - Savchenko,Andrej, AU - Kashuba,Elena, AU - Kashuba,Vladimir, AU - Snopok,Boris, PY - 2007/2/15/pubmed PY - 2007/4/14/medline PY - 2007/2/15/entrez SP - 1349 EP - 55 JF - Analytical chemistry JO - Anal Chem VL - 79 IS - 4 N2 - We propose a novel technique to detect protein-protein interactions in microarray format. The technique involves measuring scattered light under surface plasmon resonance (SPR) conditions. We have shown that the maximum scattering angle correlates with the traditionally employed reflection minimum. Panoramic scanning of scattered light under SPR conditions has all the functional advantages of the SPR technique. In addition, the proposed technique simplifies device design, increases the dynamic range of analysis, and integrates data with those from surface-plasmon field-enhanced fluorescence spectroscopy. We demonstrate the technique by showing direct protein-protein interaction between protein A and either rabbit antibodies or human serum. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/17297933/Imaging_technique_for_the_screening_of_protein_protein_interactions_using_scattered_light_under_surface_plasmon_resonance_conditions_ L2 - https://doi.org/10.1021/ac061456n DB - PRIME DP - Unbound Medicine ER -