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Gold nanoparticles as assisted matrix for determining neutral small carbohydrates through laser desorption/ionization time-of-flight mass spectrometry.
Anal Chem. 2007 Feb 15; 79(4):1626-33.AC

Abstract

A method for the analysis of small neutral carbohydrates by surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) has been developed by using bare AuNPs as matrices. In comparison with citrate-capped and DDAB-capped AuNPs, bare AuNPs can capture the analytes on their surface; therefore, small neutral carbohydrates, which are difficult to ionize by matrix-assisted desorption/ionization mass spectrometry, could be cationized very efficiently by SALDI-MS with AuNPs as matrices. By using SALDI-MS in the positive ion mode, many molecular ions are obtained from monosaccharides and disaccharides. Without derivatization, the limits of detection at a S/N ratio of 3 are 82, 41, 144, and 151 nM for ribose, glucose, cellobiose, and maltose. In comparison with conventional organic matrixes (e.g., 2,5-dihydroxybenzoic acid), bare AuNPs as SALDI matrices offer many advantages, such as easy sample preparation, high ionization efficiency, and high shot-to-shot reproducibility. To validate the applicability of our method, a calibration curve is created from urine spiked with standard glucose (0.5-10 mM). We strongly believe that this approach can potentially be applied to diagnosis and glycomics.

Authors+Show Affiliations

Department of Chemistry, National Sun Yat-sen University and National Sun Yat-Sen University-Kaohsiung Medical University Joint Center, Kaohsiung, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17297965

Citation

Su, Chih-Lin, and Wei-Lung Tseng. "Gold Nanoparticles as Assisted Matrix for Determining Neutral Small Carbohydrates Through Laser Desorption/ionization Time-of-flight Mass Spectrometry." Analytical Chemistry, vol. 79, no. 4, 2007, pp. 1626-33.
Su CL, Tseng WL. Gold nanoparticles as assisted matrix for determining neutral small carbohydrates through laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2007;79(4):1626-33.
Su, C. L., & Tseng, W. L. (2007). Gold nanoparticles as assisted matrix for determining neutral small carbohydrates through laser desorption/ionization time-of-flight mass spectrometry. Analytical Chemistry, 79(4), 1626-33.
Su CL, Tseng WL. Gold Nanoparticles as Assisted Matrix for Determining Neutral Small Carbohydrates Through Laser Desorption/ionization Time-of-flight Mass Spectrometry. Anal Chem. 2007 Feb 15;79(4):1626-33. PubMed PMID: 17297965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gold nanoparticles as assisted matrix for determining neutral small carbohydrates through laser desorption/ionization time-of-flight mass spectrometry. AU - Su,Chih-Lin, AU - Tseng,Wei-Lung, PY - 2007/2/15/pubmed PY - 2007/4/14/medline PY - 2007/2/15/entrez SP - 1626 EP - 33 JF - Analytical chemistry JO - Anal Chem VL - 79 IS - 4 N2 - A method for the analysis of small neutral carbohydrates by surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) has been developed by using bare AuNPs as matrices. In comparison with citrate-capped and DDAB-capped AuNPs, bare AuNPs can capture the analytes on their surface; therefore, small neutral carbohydrates, which are difficult to ionize by matrix-assisted desorption/ionization mass spectrometry, could be cationized very efficiently by SALDI-MS with AuNPs as matrices. By using SALDI-MS in the positive ion mode, many molecular ions are obtained from monosaccharides and disaccharides. Without derivatization, the limits of detection at a S/N ratio of 3 are 82, 41, 144, and 151 nM for ribose, glucose, cellobiose, and maltose. In comparison with conventional organic matrixes (e.g., 2,5-dihydroxybenzoic acid), bare AuNPs as SALDI matrices offer many advantages, such as easy sample preparation, high ionization efficiency, and high shot-to-shot reproducibility. To validate the applicability of our method, a calibration curve is created from urine spiked with standard glucose (0.5-10 mM). We strongly believe that this approach can potentially be applied to diagnosis and glycomics. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/17297965/Gold_nanoparticles_as_assisted_matrix_for_determining_neutral_small_carbohydrates_through_laser_desorption/ionization_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1021/ac061747w DB - PRIME DP - Unbound Medicine ER -