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Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay.
J Comb Chem. 2007 Mar-Apr; 9(2):237-44.JC

Abstract

A new type of encoded bead, which uses surface-enhanced Raman scattering (SERS), is described for multiplex immunoassays. Silver nanoparticles were embedded in sulfonated polystyrene (PS) beads via a polyol method, and they were used as SERS-active substrates. Raman-label organic compounds such as 4-methylbenzenethiol (4-MT), 2-naphthalenethiol (2-NT), and benzenethiol (BT) were then adsorbed onto the silver nanoparticles in the sulfonated PS bead. Although only three kinds of encoding have been demonstrated here, various combinations of these Raman-label organic compounds have the potential to give a large number of tags. The Raman-label-incorporated particles were then coated with a silica shell using tetraethoxyorthosilicate (TEOS) for chemical stability and biocompatibility. The resulting beads showed unique and intense Raman signals for the labeled organic compounds. We demonstrated that SERS-encoded beads could be used for multiplex detection with a model using streptavidin and p53. In our system, the binding event of target molecules and the type of ligand can be simultaneously recognized by Raman spectroscopy using a single laser-line excitation (514.5 nm).

Authors+Show Affiliations

Organic Synthesis Laboratory, School of Chemical and Biological Engineering, Seoul National University, Seoul 151-747, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17298100

Citation

Jun, Bong-Hyun, et al. "Surface-enhanced Raman Spectroscopic-encoded Beads for Multiplex Immunoassay." Journal of Combinatorial Chemistry, vol. 9, no. 2, 2007, pp. 237-44.
Jun BH, Kim JH, Park H, et al. Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay. J Comb Chem. 2007;9(2):237-44.
Jun, B. H., Kim, J. H., Park, H., Kim, J. S., Yu, K. N., Lee, S. M., Choi, H., Kwak, S. Y., Kim, Y. K., Jeong, D. H., Cho, M. H., & Lee, Y. S. (2007). Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay. Journal of Combinatorial Chemistry, 9(2), 237-44.
Jun BH, et al. Surface-enhanced Raman Spectroscopic-encoded Beads for Multiplex Immunoassay. J Comb Chem. 2007 Mar-Apr;9(2):237-44. PubMed PMID: 17298100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay. AU - Jun,Bong-Hyun, AU - Kim,Jong-Ho, AU - Park,Hyunmi, AU - Kim,Jun-Sung, AU - Yu,Kyeong-Nam, AU - Lee,Sang-Myung, AU - Choi,Heejeong, AU - Kwak,Seon-Yeong, AU - Kim,Yong-Kweon, AU - Jeong,Dae Hong, AU - Cho,Myung-Haing, AU - Lee,Yoon-Sik, Y1 - 2007/02/14/ PY - 2007/2/15/pubmed PY - 2007/5/16/medline PY - 2007/2/15/entrez SP - 237 EP - 44 JF - Journal of combinatorial chemistry JO - J Comb Chem VL - 9 IS - 2 N2 - A new type of encoded bead, which uses surface-enhanced Raman scattering (SERS), is described for multiplex immunoassays. Silver nanoparticles were embedded in sulfonated polystyrene (PS) beads via a polyol method, and they were used as SERS-active substrates. Raman-label organic compounds such as 4-methylbenzenethiol (4-MT), 2-naphthalenethiol (2-NT), and benzenethiol (BT) were then adsorbed onto the silver nanoparticles in the sulfonated PS bead. Although only three kinds of encoding have been demonstrated here, various combinations of these Raman-label organic compounds have the potential to give a large number of tags. The Raman-label-incorporated particles were then coated with a silica shell using tetraethoxyorthosilicate (TEOS) for chemical stability and biocompatibility. The resulting beads showed unique and intense Raman signals for the labeled organic compounds. We demonstrated that SERS-encoded beads could be used for multiplex detection with a model using streptavidin and p53. In our system, the binding event of target molecules and the type of ligand can be simultaneously recognized by Raman spectroscopy using a single laser-line excitation (514.5 nm). SN - 1520-4766 UR - https://www.unboundmedicine.com/medline/citation/17298100/Surface_enhanced_Raman_spectroscopic_encoded_beads_for_multiplex_immunoassay_ L2 - https://doi.org/10.1021/cc0600831 DB - PRIME DP - Unbound Medicine ER -