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Drying a tuberculosis vaccine without freezing.
Proc Natl Acad Sci U S A 2007; 104(8):2591-5PN

Abstract

With the increasing incidence of tuberculosis and drug resistant disease in developing countries due to HIV/AIDS, there is a need for vaccines that are more effective than the present bacillus Calmette-Guérin (BCG) vaccine. We demonstrate that BCG vaccine can be dried without traditional freezing and maintained with remarkable refrigerated and room-temperature stability for months through spray drying. Studies with a model Mycobacterium (Mycobacterium smegmatis) revealed that by removing salts and cryoprotectant (e.g., glycerol) from bacterial suspensions, the significant osmotic pressures that are normally produced on bacterial membranes through droplet drying can be reduced sufficiently to minimize loss of viability on drying by up to 2 orders of magnitude. By placing the bacteria in a matrix of leucine, high-yield, free-flowing, "vial-fillable" powders of bacteria (including M. smegmatis and M. bovis BCG) can be produced. These powders show relatively minor losses of activity after maintenance at 4 degrees C and 25 degrees C up to and beyond 4 months. Comparisons with lyophilized material prepared both with the same formulation and with a commercial formulation reveal that the spray-dried BCG has better overall viability on drying.

Authors+Show Affiliations

Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17299039

Citation

Wong, Yun-Ling, et al. "Drying a Tuberculosis Vaccine Without Freezing." Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 8, 2007, pp. 2591-5.
Wong YL, Sampson S, Germishuizen WA, et al. Drying a tuberculosis vaccine without freezing. Proc Natl Acad Sci USA. 2007;104(8):2591-5.
Wong, Y. L., Sampson, S., Germishuizen, W. A., Goonesekera, S., Caponetti, G., Sadoff, J., ... Edwards, D. (2007). Drying a tuberculosis vaccine without freezing. Proceedings of the National Academy of Sciences of the United States of America, 104(8), pp. 2591-5.
Wong YL, et al. Drying a Tuberculosis Vaccine Without Freezing. Proc Natl Acad Sci USA. 2007 Feb 20;104(8):2591-5. PubMed PMID: 17299039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Drying a tuberculosis vaccine without freezing. AU - Wong,Yun-Ling, AU - Sampson,Samantha, AU - Germishuizen,Willem Andreas, AU - Goonesekera,Sunali, AU - Caponetti,Giovanni, AU - Sadoff,Jerry, AU - Bloom,Barry R, AU - Edwards,David, Y1 - 2007/02/13/ PY - 2007/2/15/pubmed PY - 2007/4/14/medline PY - 2007/2/15/entrez SP - 2591 EP - 5 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 104 IS - 8 N2 - With the increasing incidence of tuberculosis and drug resistant disease in developing countries due to HIV/AIDS, there is a need for vaccines that are more effective than the present bacillus Calmette-Guérin (BCG) vaccine. We demonstrate that BCG vaccine can be dried without traditional freezing and maintained with remarkable refrigerated and room-temperature stability for months through spray drying. Studies with a model Mycobacterium (Mycobacterium smegmatis) revealed that by removing salts and cryoprotectant (e.g., glycerol) from bacterial suspensions, the significant osmotic pressures that are normally produced on bacterial membranes through droplet drying can be reduced sufficiently to minimize loss of viability on drying by up to 2 orders of magnitude. By placing the bacteria in a matrix of leucine, high-yield, free-flowing, "vial-fillable" powders of bacteria (including M. smegmatis and M. bovis BCG) can be produced. These powders show relatively minor losses of activity after maintenance at 4 degrees C and 25 degrees C up to and beyond 4 months. Comparisons with lyophilized material prepared both with the same formulation and with a commercial formulation reveal that the spray-dried BCG has better overall viability on drying. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/17299039/Drying_a_tuberculosis_vaccine_without_freezing_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=17299039 DB - PRIME DP - Unbound Medicine ER -