Tags

Type your tag names separated by a space and hit enter

Smoking modulates interleukin-6:interleukin-10 and RANKL:osteoprotegerin ratios in the periodontal tissues.
J Periodontal Res. 2007 Apr; 42(2):184-91.JP

Abstract

BACKGROUND AND OBJECTIVE

This study evaluated the effect of smoking on the gene expression of interleukin-1alpha, -1ra, -6, -8 and -10, tumor necrosis factor-alpha, matrix metalloproteinase (MMP)-2 and -8, receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin, in sites with periodontitis.

MATERIAL AND METHODS

Gingival biopsies were divided into three groups: the healthy group (periodontally healthy subjects; n=10); the periodontitis group [subjects with severe chronic periodontitis who never smoked (probing depth>or=7 mm) (n=25)]; and the smoking group (subjects diagnosed with severe chronic periodontitis who smoked>or=1 pack per day for at least 10 years; n=25). Gene and protein expressions were analyzed by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.

RESULTS

Data analysis demonstrated that, except for MMP-8 and osteoprotegerin, the levels of all factors were increased by inflammation (p<0.001). The levels of interleukin-1alpha, -1ra, -6 and -8, and RANKL, were higher in smokers with periodontitis compared with controls, whereas the levels of interleukin-10, MMP-8 and osteoprotegerin were lower (p<0.001). Smoking lowered the levels of interleukin-1alpha, -8, -10, tumor necrosis factor-alpha, MMP-8 and osteoprotegerin, and increased the levels of interleukin-6 and -1ra in sites with a comparable type of periodontitis (p<0.001).

CONCLUSION

In conclusion, smoking modulates gene expression in the periodontium, and the influence of smoking on periodontal disease may involve effects of interleukin-6:interleukin-10 and RANKL:osteoprotegerin ratios.

Authors+Show Affiliations

Department of Prosthodontics/Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17305878

Citation

César-Neto, J B., et al. "Smoking Modulates Interleukin-6:interleukin-10 and RANKL:osteoprotegerin Ratios in the Periodontal Tissues." Journal of Periodontal Research, vol. 42, no. 2, 2007, pp. 184-91.
César-Neto JB, Duarte PM, de Oliveira MC, et al. Smoking modulates interleukin-6:interleukin-10 and RANKL:osteoprotegerin ratios in the periodontal tissues. J Periodontal Res. 2007;42(2):184-91.
César-Neto, J. B., Duarte, P. M., de Oliveira, M. C., Tambeli, C. H., Sallum, E. A., & Nociti, F. H. (2007). Smoking modulates interleukin-6:interleukin-10 and RANKL:osteoprotegerin ratios in the periodontal tissues. Journal of Periodontal Research, 42(2), 184-91.
César-Neto JB, et al. Smoking Modulates Interleukin-6:interleukin-10 and RANKL:osteoprotegerin Ratios in the Periodontal Tissues. J Periodontal Res. 2007;42(2):184-91. PubMed PMID: 17305878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Smoking modulates interleukin-6:interleukin-10 and RANKL:osteoprotegerin ratios in the periodontal tissues. AU - César-Neto,J B, AU - Duarte,P M, AU - de Oliveira,M C G, AU - Tambeli,C H, AU - Sallum,E A, AU - Nociti,F H,Jr PY - 2007/2/20/pubmed PY - 2007/4/17/medline PY - 2007/2/20/entrez SP - 184 EP - 91 JF - Journal of periodontal research JO - J Periodontal Res VL - 42 IS - 2 N2 - BACKGROUND AND OBJECTIVE: This study evaluated the effect of smoking on the gene expression of interleukin-1alpha, -1ra, -6, -8 and -10, tumor necrosis factor-alpha, matrix metalloproteinase (MMP)-2 and -8, receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin, in sites with periodontitis. MATERIAL AND METHODS: Gingival biopsies were divided into three groups: the healthy group (periodontally healthy subjects; n=10); the periodontitis group [subjects with severe chronic periodontitis who never smoked (probing depth>or=7 mm) (n=25)]; and the smoking group (subjects diagnosed with severe chronic periodontitis who smoked>or=1 pack per day for at least 10 years; n=25). Gene and protein expressions were analyzed by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. RESULTS: Data analysis demonstrated that, except for MMP-8 and osteoprotegerin, the levels of all factors were increased by inflammation (p<0.001). The levels of interleukin-1alpha, -1ra, -6 and -8, and RANKL, were higher in smokers with periodontitis compared with controls, whereas the levels of interleukin-10, MMP-8 and osteoprotegerin were lower (p<0.001). Smoking lowered the levels of interleukin-1alpha, -8, -10, tumor necrosis factor-alpha, MMP-8 and osteoprotegerin, and increased the levels of interleukin-6 and -1ra in sites with a comparable type of periodontitis (p<0.001). CONCLUSION: In conclusion, smoking modulates gene expression in the periodontium, and the influence of smoking on periodontal disease may involve effects of interleukin-6:interleukin-10 and RANKL:osteoprotegerin ratios. SN - 0022-3484 UR - https://www.unboundmedicine.com/medline/citation/17305878/Smoking_modulates_interleukin_6:interleukin_10_and_RANKL:osteoprotegerin_ratios_in_the_periodontal_tissues_ L2 - https://doi.org/10.1111/j.1600-0765.2006.00934.x DB - PRIME DP - Unbound Medicine ER -