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Gene expressions and roles of matrix metalloproteinases-8 and tissue inhibitor of metalloproteinases-1 in hyperoxia-induced pulmonary fibrosis in neonatal rats.
Zhongguo Dang Dai Er Ke Za Zhi. 2007 Feb; 9(1):1-5.ZD

Abstract

OBJECTIVE

Extracellular matrix (ECM) deposition is a major reason of pulmonary fibrosis in hyperoxia-induced lung injury. However, the relevant mechanism has not been identified. This study examined the gene expressions of matrix metalloproteinases-8 (MMP-8, a catabolic enzyme of type I collagen) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in neonatal rats with hyperoxia-induced pulmonary injury in order to explore the role of MMP-8 and TIMP-1 in pulmonary fibrosis.

METHODS

Eighty term newborn rats were randomly exposed to hyperoxia (FiO2=0.90, hyperoxia group)and to room air (FiO2=0.21, control group)(n=40 each). Lung injury was induced by hyperoxia exposure. The content of type I collagen and the expressions of type I collagen protein and MMP-1 mRNA and TIMP-1 mRNA were assayed with enzyme linked immunoadsorbent (ELISA), immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) respectively on days 1, 3, 7, 14 and 21 after exposure.

RESULTS

The content of type I collagen and the expression of type I collagen protein in the hyperoxia group were statistically higher than those in the control group at 14 and 21 days post-exposure. The MMP-8 mRNA expression decreased while the TIMP-1 mRNA expression increased significantly in the hyperoxia group as compared to the control group at 14 and 21 days post-exposure.

CONCLUSIONS

Hyperoxia exposure down-regulates MMP-8 mRNA expression and up-regulates TIMP-1 mRNA expression. This results in a reduction of ECM degradation, thereby ECM deposition occurs in lung tissue, which may be an important mechanism of pulmonary fibrosis following hyperoxia-induced lung injury.

Authors+Show Affiliations

Fu JH
Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, China.
Xue XD
No affiliation info available

MeSH

AnimalsAnimals, NewbornChronic DiseaseCollagen Type IFemaleHyperoxiaMaleMatrix Metalloproteinase 8Pulmonary FibrosisRNA, MessengerRatsTissue Inhibitor of Metalloproteinase-1

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17306066

Citation

Fu, Jian-Hua, and Xin-Dong Xue. "Gene Expressions and Roles of Matrix Metalloproteinases-8 and Tissue Inhibitor of Metalloproteinases-1 in Hyperoxia-induced Pulmonary Fibrosis in Neonatal Rats." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 9, no. 1, 2007, pp. 1-5.
Fu JH, Xue XD. Gene expressions and roles of matrix metalloproteinases-8 and tissue inhibitor of metalloproteinases-1 in hyperoxia-induced pulmonary fibrosis in neonatal rats. Zhongguo Dang Dai Er Ke Za Zhi. 2007;9(1):1-5.
Fu, J. H., & Xue, X. D. (2007). Gene expressions and roles of matrix metalloproteinases-8 and tissue inhibitor of metalloproteinases-1 in hyperoxia-induced pulmonary fibrosis in neonatal rats. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 9(1), 1-5.
Fu JH, Xue XD. Gene Expressions and Roles of Matrix Metalloproteinases-8 and Tissue Inhibitor of Metalloproteinases-1 in Hyperoxia-induced Pulmonary Fibrosis in Neonatal Rats. Zhongguo Dang Dai Er Ke Za Zhi. 2007;9(1):1-5. PubMed PMID: 17306066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expressions and roles of matrix metalloproteinases-8 and tissue inhibitor of metalloproteinases-1 in hyperoxia-induced pulmonary fibrosis in neonatal rats. AU - Fu,Jian-Hua, AU - Xue,Xin-Dong, PY - 2007/2/20/pubmed PY - 2007/3/21/medline PY - 2007/2/20/entrez SP - 1 EP - 5 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 9 IS - 1 N2 - OBJECTIVE: Extracellular matrix (ECM) deposition is a major reason of pulmonary fibrosis in hyperoxia-induced lung injury. However, the relevant mechanism has not been identified. This study examined the gene expressions of matrix metalloproteinases-8 (MMP-8, a catabolic enzyme of type I collagen) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in neonatal rats with hyperoxia-induced pulmonary injury in order to explore the role of MMP-8 and TIMP-1 in pulmonary fibrosis. METHODS: Eighty term newborn rats were randomly exposed to hyperoxia (FiO2=0.90, hyperoxia group)and to room air (FiO2=0.21, control group)(n=40 each). Lung injury was induced by hyperoxia exposure. The content of type I collagen and the expressions of type I collagen protein and MMP-1 mRNA and TIMP-1 mRNA were assayed with enzyme linked immunoadsorbent (ELISA), immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) respectively on days 1, 3, 7, 14 and 21 after exposure. RESULTS: The content of type I collagen and the expression of type I collagen protein in the hyperoxia group were statistically higher than those in the control group at 14 and 21 days post-exposure. The MMP-8 mRNA expression decreased while the TIMP-1 mRNA expression increased significantly in the hyperoxia group as compared to the control group at 14 and 21 days post-exposure. CONCLUSIONS: Hyperoxia exposure down-regulates MMP-8 mRNA expression and up-regulates TIMP-1 mRNA expression. This results in a reduction of ECM degradation, thereby ECM deposition occurs in lung tissue, which may be an important mechanism of pulmonary fibrosis following hyperoxia-induced lung injury. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/17306066/Gene_expressions_and_roles_of_matrix_metalloproteinases_8_and_tissue_inhibitor_of_metalloproteinases_1_in_hyperoxia_induced_pulmonary_fibrosis_in_neonatal_rats_ L2 - https://medlineplus.gov/pulmonaryfibrosis.html DB - PRIME DP - Unbound Medicine ER -

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