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Receptor activator of NF-kappaB ligand induces the expression of carbonic anhydrase II, cathepsin K, and matrix metalloproteinase-9 in osteoclast precursor RAW264.7 cells.
Life Sci. 2007 Mar 13; 80(14):1311-8.LS

Abstract

Interleukin-1 (IL-1) is a proinflammatory cytokine that is a potent stimulator of bone resorption and an inhibitor of bone formation, whereas macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-kappaB (RANK) ligand (RANKL) are essential and sufficient for osteoclast differentiation. Recently, we showed that IL-1alpha affects mineralized nodule formation in vitro and halts bone matrix turnover. We also showed that IL-1alpha stimulates osteoclast formation via the interaction of RANKL with RANK by increasing M-CSF and prostaglandin E(2) and decreasing osteoprotegerin. Here, we examined the effects of IL-1alpha or RANKL and/or M-CSF in the presence of IL-1alpha on the expression of carbonic anhydrase II (CAII), cathepsin K, matrix metalloproteinase-9 (MMP-9), RANK, M-CSF receptor (c-fms), and c-fos transcription factor using RAW264.7 cells as osteoclast precursors. Cells were cultured for up to 14 days in 0 or 100 U/ml IL-1alpha and either 50 ng/ml RANKL, 10 ng/ml M-CSF, or 50 ng/ml RANKL+10 ng/ml M-CSF in the presence of 100 U/ml IL-1alpha. The formation of osteoclast-like cells was estimated using tartrate-resistant acid phosphatase staining. Expression of the genes coding for the six proteins of interest was determined using real-time PCR, and the expression of the three enzymes was examined using Western blotting or ELISA. In the presence of IL-1alpha, expression of CAII, cathepsin K, and MMP-9 was markedly increased in cells cultured with RANKL or M-CSF+RANKL, whereas expression was difficult to detect in cells cultured with IL-1alpha alone and M-CSF. RANK and c-fos expression was also increased in cells cultured with RANKL or M-CSF+RANKL in the presence of IL-1alpha, whereas c-fms expression did not change. These results indicate that the expression of CAII, cathepsin K, and MMP-9 in RAW264.7 cells is not induced by M-CSF, but by RANKL in the presence of IL-1alpha.

Authors+Show Affiliations

Nihon University Graduate School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17306833

Citation

Fujisaki, Kyosuke, et al. "Receptor Activator of NF-kappaB Ligand Induces the Expression of Carbonic Anhydrase II, Cathepsin K, and Matrix Metalloproteinase-9 in Osteoclast Precursor RAW264.7 Cells." Life Sciences, vol. 80, no. 14, 2007, pp. 1311-8.
Fujisaki K, Tanabe N, Suzuki N, et al. Receptor activator of NF-kappaB ligand induces the expression of carbonic anhydrase II, cathepsin K, and matrix metalloproteinase-9 in osteoclast precursor RAW264.7 cells. Life Sci. 2007;80(14):1311-8.
Fujisaki, K., Tanabe, N., Suzuki, N., Kawato, T., Takeichi, O., Tsuzukibashi, O., Makimura, M., Ito, K., & Maeno, M. (2007). Receptor activator of NF-kappaB ligand induces the expression of carbonic anhydrase II, cathepsin K, and matrix metalloproteinase-9 in osteoclast precursor RAW264.7 cells. Life Sciences, 80(14), 1311-8.
Fujisaki K, et al. Receptor Activator of NF-kappaB Ligand Induces the Expression of Carbonic Anhydrase II, Cathepsin K, and Matrix Metalloproteinase-9 in Osteoclast Precursor RAW264.7 Cells. Life Sci. 2007 Mar 13;80(14):1311-8. PubMed PMID: 17306833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Receptor activator of NF-kappaB ligand induces the expression of carbonic anhydrase II, cathepsin K, and matrix metalloproteinase-9 in osteoclast precursor RAW264.7 cells. AU - Fujisaki,Kyosuke, AU - Tanabe,Natsuko, AU - Suzuki,Naoto, AU - Kawato,Takayuki, AU - Takeichi,Osamu, AU - Tsuzukibashi,Osamu, AU - Makimura,Masaharu, AU - Ito,Koichi, AU - Maeno,Masao, Y1 - 2007/01/23/ PY - 2006/09/05/received PY - 2006/10/31/revised PY - 2006/12/15/accepted PY - 2007/2/20/pubmed PY - 2007/4/14/medline PY - 2007/2/20/entrez SP - 1311 EP - 8 JF - Life sciences JO - Life Sci VL - 80 IS - 14 N2 - Interleukin-1 (IL-1) is a proinflammatory cytokine that is a potent stimulator of bone resorption and an inhibitor of bone formation, whereas macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-kappaB (RANK) ligand (RANKL) are essential and sufficient for osteoclast differentiation. Recently, we showed that IL-1alpha affects mineralized nodule formation in vitro and halts bone matrix turnover. We also showed that IL-1alpha stimulates osteoclast formation via the interaction of RANKL with RANK by increasing M-CSF and prostaglandin E(2) and decreasing osteoprotegerin. Here, we examined the effects of IL-1alpha or RANKL and/or M-CSF in the presence of IL-1alpha on the expression of carbonic anhydrase II (CAII), cathepsin K, matrix metalloproteinase-9 (MMP-9), RANK, M-CSF receptor (c-fms), and c-fos transcription factor using RAW264.7 cells as osteoclast precursors. Cells were cultured for up to 14 days in 0 or 100 U/ml IL-1alpha and either 50 ng/ml RANKL, 10 ng/ml M-CSF, or 50 ng/ml RANKL+10 ng/ml M-CSF in the presence of 100 U/ml IL-1alpha. The formation of osteoclast-like cells was estimated using tartrate-resistant acid phosphatase staining. Expression of the genes coding for the six proteins of interest was determined using real-time PCR, and the expression of the three enzymes was examined using Western blotting or ELISA. In the presence of IL-1alpha, expression of CAII, cathepsin K, and MMP-9 was markedly increased in cells cultured with RANKL or M-CSF+RANKL, whereas expression was difficult to detect in cells cultured with IL-1alpha alone and M-CSF. RANK and c-fos expression was also increased in cells cultured with RANKL or M-CSF+RANKL in the presence of IL-1alpha, whereas c-fms expression did not change. These results indicate that the expression of CAII, cathepsin K, and MMP-9 in RAW264.7 cells is not induced by M-CSF, but by RANKL in the presence of IL-1alpha. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/17306833/Receptor_activator_of_NF_kappaB_ligand_induces_the_expression_of_carbonic_anhydrase_II_cathepsin_K_and_matrix_metalloproteinase_9_in_osteoclast_precursor_RAW264_7_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(07)00031-8 DB - PRIME DP - Unbound Medicine ER -