Tags

Type your tag names separated by a space and hit enter

The effect of influenza vaccination on IL2 production in healthy elderly: implications for current vaccination practices.

Abstract

Age-related senescence of T-cell mediated responses is well recognized. This study was designed to determine how aging affects the T-cell mediated Interleukin 2 (IL2) response to influenza vaccination. A group of healthy elderly individuals were compared to a control group of healthy young adults for their response to the 1990 influenza vaccine. Cultures of peripheral blood mononuclear cells (PBMC) were prepared from venous blood samples taken prevaccination (pre) and 8 and 12 weeks post-vaccination (post). PBMC cultures stimulated with inactivated A/Shanghai/16/89 (contained in the 1990 vaccine) and A/Philippine/2/82 (not contained in the vaccine) were assayed for peak IL2 activity. We find that after influenza vaccination, there was an insignificant increase in IL2 activity when PBMC from the young control group were stimulated with A/Shanghai/16/89 (pre, 5.14 U/mL/10(6) PBMC; post, 6.64 U/mL/10(6) PBMC) but there was a significant increase in IL2 activity when stimulated with A/Phillippine/2/82 (pre, 1.5 U/mL/10(6) PBMC; post, 8.3 U/mL/10(6) PBMC). In similar cultures of PBMC from the elderly group, there was a significant increase in IL2 response to both A/Shanghai/16/89 (pre, 1.6 U/mL/10(6) PBMC; post, 3.5 U/mL/10(6) PBMC) and A/Philippine/2/82 (pre, 0.86 U/mL/10(6) PBMC; post, 8.3 U/mL/10(6) PBMC). Measurements of CD4+/CD8+ populations were not affected by vaccination and were not significantly different in the two groups. Subgroup analysis of the elderly group revealed that previous influenza vaccination in 1989 did not significantly affect IL2 levels measured in the present study. This study shows that in healthy elderly, influenza vaccination effectively restores IL2 activity to normal. There appears to be an age-related decrease in the duration of T-cell memory.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

,

University of Alberta, Edmonton.

, , , , ,

Source

Journal of gerontology 47:1 1992 Jan pg M3-8

MeSH

Adult
Aged
Aged, 80 and over
Aging
CD4 Antigens
CD8 Antigens
Female
Humans
In Vitro Techniques
Influenza A virus
Influenza Vaccines
Interleukin-2
Leukocytes, Mononuclear
Lymphocyte Activation
Male
Middle Aged
Time Factors
Vaccination

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1730850

Citation

McElhaney, J E., et al. "The Effect of Influenza Vaccination On IL2 Production in Healthy Elderly: Implications for Current Vaccination Practices." Journal of Gerontology, vol. 47, no. 1, 1992, pp. M3-8.
McElhaney JE, Meneilly GS, Beattie BL, et al. The effect of influenza vaccination on IL2 production in healthy elderly: implications for current vaccination practices. J Gerontol. 1992;47(1):M3-8.
McElhaney, J. E., Meneilly, G. S., Beattie, B. L., Helgason, C. D., Lee, S. F., Devine, R. D., & Bleackley, R. C. (1992). The effect of influenza vaccination on IL2 production in healthy elderly: implications for current vaccination practices. Journal of Gerontology, 47(1), pp. M3-8.
McElhaney JE, et al. The Effect of Influenza Vaccination On IL2 Production in Healthy Elderly: Implications for Current Vaccination Practices. J Gerontol. 1992;47(1):M3-8. PubMed PMID: 1730850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of influenza vaccination on IL2 production in healthy elderly: implications for current vaccination practices. AU - McElhaney,J E, AU - Meneilly,G S, AU - Beattie,B L, AU - Helgason,C D, AU - Lee,S F, AU - Devine,R D, AU - Bleackley,R C, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - M3 EP - 8 JF - Journal of gerontology JO - J Gerontol VL - 47 IS - 1 N2 - Age-related senescence of T-cell mediated responses is well recognized. This study was designed to determine how aging affects the T-cell mediated Interleukin 2 (IL2) response to influenza vaccination. A group of healthy elderly individuals were compared to a control group of healthy young adults for their response to the 1990 influenza vaccine. Cultures of peripheral blood mononuclear cells (PBMC) were prepared from venous blood samples taken prevaccination (pre) and 8 and 12 weeks post-vaccination (post). PBMC cultures stimulated with inactivated A/Shanghai/16/89 (contained in the 1990 vaccine) and A/Philippine/2/82 (not contained in the vaccine) were assayed for peak IL2 activity. We find that after influenza vaccination, there was an insignificant increase in IL2 activity when PBMC from the young control group were stimulated with A/Shanghai/16/89 (pre, 5.14 U/mL/10(6) PBMC; post, 6.64 U/mL/10(6) PBMC) but there was a significant increase in IL2 activity when stimulated with A/Phillippine/2/82 (pre, 1.5 U/mL/10(6) PBMC; post, 8.3 U/mL/10(6) PBMC). In similar cultures of PBMC from the elderly group, there was a significant increase in IL2 response to both A/Shanghai/16/89 (pre, 1.6 U/mL/10(6) PBMC; post, 3.5 U/mL/10(6) PBMC) and A/Philippine/2/82 (pre, 0.86 U/mL/10(6) PBMC; post, 8.3 U/mL/10(6) PBMC). Measurements of CD4+/CD8+ populations were not affected by vaccination and were not significantly different in the two groups. Subgroup analysis of the elderly group revealed that previous influenza vaccination in 1989 did not significantly affect IL2 levels measured in the present study. This study shows that in healthy elderly, influenza vaccination effectively restores IL2 activity to normal. There appears to be an age-related decrease in the duration of T-cell memory.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0022-1422 UR - https://www.unboundmedicine.com/medline/citation/1730850/The_effect_of_influenza_vaccination_on_IL2_production_in_healthy_elderly:_implications_for_current_vaccination_practices_ L2 - https://medlineplus.gov/seniorshealth.html DB - PRIME DP - Unbound Medicine ER -