Tags

Type your tag names separated by a space and hit enter

Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina.
J Comp Neurol. 2007 Apr 20; 501(6):891-903.JC

Abstract

The hyperpolarization-activated and cyclic nucleotide-gated (HCN) channel isoforms HCN1, HCN2, and HCN4 were localized by immunofluorescence in the rat retina. Double labeling with the vesicular glutamate transporter (VGLUT1) was used to identify bipolar cell axon terminals in the inner retina. The HCN1 channel was localized to two cell types with differing intracellular distributions, insofar as staining was seen in the dendrites of a putative OFF-type cone bipolar cell and in the axon terminals of an ON-type bipolar that ramifies in stratum 3 (s3) of the inner plexiform layer (IPL). Staining for HCN4 was seen in two sets of bipolar axon terminals located in s2 and s3 and positioned between the two bands of choline acetyltransferase (ChAT) staining. The cells that ramify in s2 were identified as type 3 cone bipolar cells and the cells that ramify in s3 cells as a subclass of type 5 cone bipolars. The latter group, designated here as type 5b, exhibit diffuse axon terminals and can be distinguished from the narrowly stratifying type 5a cells. Double labeling showed that type 5b cone bipolar cells express both HCN1 and HCN4 as well as HCN2. Superposition of HCN channel labeling with VGLUT1 staining confirmed the presence of a cone bipolar cell whose terminals ramify in the same stratum of the IPL as type 5b cells but that do not express these HCN channels.

Authors+Show Affiliations

Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17311321

Citation

Fyk-Kolodziej, Bozena, and Roberta G. Pourcho. "Differential Distribution of Hyperpolarization-activated and Cyclic Nucleotide-gated Channels in Cone Bipolar Cells of the Rat Retina." The Journal of Comparative Neurology, vol. 501, no. 6, 2007, pp. 891-903.
Fyk-Kolodziej B, Pourcho RG. Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina. J Comp Neurol. 2007;501(6):891-903.
Fyk-Kolodziej, B., & Pourcho, R. G. (2007). Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina. The Journal of Comparative Neurology, 501(6), 891-903.
Fyk-Kolodziej B, Pourcho RG. Differential Distribution of Hyperpolarization-activated and Cyclic Nucleotide-gated Channels in Cone Bipolar Cells of the Rat Retina. J Comp Neurol. 2007 Apr 20;501(6):891-903. PubMed PMID: 17311321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina. AU - Fyk-Kolodziej,Bozena, AU - Pourcho,Roberta G, PY - 2007/2/22/pubmed PY - 2007/4/27/medline PY - 2007/2/22/entrez SP - 891 EP - 903 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 501 IS - 6 N2 - The hyperpolarization-activated and cyclic nucleotide-gated (HCN) channel isoforms HCN1, HCN2, and HCN4 were localized by immunofluorescence in the rat retina. Double labeling with the vesicular glutamate transporter (VGLUT1) was used to identify bipolar cell axon terminals in the inner retina. The HCN1 channel was localized to two cell types with differing intracellular distributions, insofar as staining was seen in the dendrites of a putative OFF-type cone bipolar cell and in the axon terminals of an ON-type bipolar that ramifies in stratum 3 (s3) of the inner plexiform layer (IPL). Staining for HCN4 was seen in two sets of bipolar axon terminals located in s2 and s3 and positioned between the two bands of choline acetyltransferase (ChAT) staining. The cells that ramify in s2 were identified as type 3 cone bipolar cells and the cells that ramify in s3 cells as a subclass of type 5 cone bipolars. The latter group, designated here as type 5b, exhibit diffuse axon terminals and can be distinguished from the narrowly stratifying type 5a cells. Double labeling showed that type 5b cone bipolar cells express both HCN1 and HCN4 as well as HCN2. Superposition of HCN channel labeling with VGLUT1 staining confirmed the presence of a cone bipolar cell whose terminals ramify in the same stratum of the IPL as type 5b cells but that do not express these HCN channels. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/17311321/Differential_distribution_of_hyperpolarization_activated_and_cyclic_nucleotide_gated_channels_in_cone_bipolar_cells_of_the_rat_retina_ L2 - https://doi.org/10.1002/cne.21287 DB - PRIME DP - Unbound Medicine ER -