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Raman microspectroscopic analysis of triterpenoids found in plant cuticles.
Appl Spectrosc 2007; 61(1):32-7AS

Abstract

The above-ground organs of plants are covered by a cuticle, an extracellular membrane performing important physiological and ecological functions, that consists of the fatty acid-derived polymer cutin and waxes. In the cuticular wax of many species, including the leaves of Prunus laurocerasus, triterpenoids are found at high concentrations. This paper investigates the potential of Raman microspectroscopy for the simultaneous detection of structurally similar triterpenoids in plant cuticles. Relative composition analysis was first performed on artificial triterpenoid mixtures consisting of alpha-amyrin and oleanolic acid, as well as oleanolic acid and ursolic acid, the two triterpenoids abundantly found in the cuticles of P. laurocerasus. The different triterpenoids could be distinguished in the mixture spectra and the resulting calculated triterpenoid ratios were consistent with the expected values. Qualitative analysis of the Raman spectra of P. laurocerasus cuticle demonstrated the in situ detectability of the triterpenoids using this approach. It is shown here that Raman microspectroscopy has the potential to provide useful information concerning the spatial distribution of some key chemical components of plant cuticles. This technique thus offers a valuable complement to the current standard analytical methods used for analyzing the bulk composition of plant cuticles.

Authors+Show Affiliations

Department of Chemistry, The University of British Columbia, 6174 University Boulevard, Vancouver, BC, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17311714

Citation

Yu, Marcia M L., et al. "Raman Microspectroscopic Analysis of Triterpenoids Found in Plant Cuticles." Applied Spectroscopy, vol. 61, no. 1, 2007, pp. 32-7.
Yu MM, Schulze HG, Jetter R, et al. Raman microspectroscopic analysis of triterpenoids found in plant cuticles. Appl Spectrosc. 2007;61(1):32-7.
Yu, M. M., Schulze, H. G., Jetter, R., Blades, M. W., & Turner, R. F. (2007). Raman microspectroscopic analysis of triterpenoids found in plant cuticles. Applied Spectroscopy, 61(1), pp. 32-7.
Yu MM, et al. Raman Microspectroscopic Analysis of Triterpenoids Found in Plant Cuticles. Appl Spectrosc. 2007;61(1):32-7. PubMed PMID: 17311714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Raman microspectroscopic analysis of triterpenoids found in plant cuticles. AU - Yu,Marcia M L, AU - Schulze,H Georg, AU - Jetter,Reinhard, AU - Blades,Michael W, AU - Turner,Robin F B, PY - 2007/2/22/pubmed PY - 2007/3/21/medline PY - 2007/2/22/entrez SP - 32 EP - 7 JF - Applied spectroscopy JO - Appl Spectrosc VL - 61 IS - 1 N2 - The above-ground organs of plants are covered by a cuticle, an extracellular membrane performing important physiological and ecological functions, that consists of the fatty acid-derived polymer cutin and waxes. In the cuticular wax of many species, including the leaves of Prunus laurocerasus, triterpenoids are found at high concentrations. This paper investigates the potential of Raman microspectroscopy for the simultaneous detection of structurally similar triterpenoids in plant cuticles. Relative composition analysis was first performed on artificial triterpenoid mixtures consisting of alpha-amyrin and oleanolic acid, as well as oleanolic acid and ursolic acid, the two triterpenoids abundantly found in the cuticles of P. laurocerasus. The different triterpenoids could be distinguished in the mixture spectra and the resulting calculated triterpenoid ratios were consistent with the expected values. Qualitative analysis of the Raman spectra of P. laurocerasus cuticle demonstrated the in situ detectability of the triterpenoids using this approach. It is shown here that Raman microspectroscopy has the potential to provide useful information concerning the spatial distribution of some key chemical components of plant cuticles. This technique thus offers a valuable complement to the current standard analytical methods used for analyzing the bulk composition of plant cuticles. SN - 0003-7028 UR - https://www.unboundmedicine.com/medline/citation/17311714/Raman_microspectroscopic_analysis_of_triterpenoids_found_in_plant_cuticles_ L2 - http://journals.sagepub.com/doi/full/10.1366/000370207779701352?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -