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Skeletal muscle mitochondrial FAT/CD36 content and palmitate oxidation are not decreased in obese women.
Am J Physiol Endocrinol Metab 2007; 292(6):E1782-9AJ

Abstract

A reduction in fatty acid oxidation has been associated with lipid accumulation and insulin resistance in the skeletal muscle of obese individuals. We examined whether this decrease in fatty acid oxidation was attributable to a reduction in muscle mitochondrial content and/or a dysfunction in fatty acid oxidation within mitochondria obtained from skeletal muscle of age-matched, lean [body mass index (BMI) = 23.3 +/- 0.7 kg/m2] and obese women (BMI = 37.6 +/- 2.2 kg/m2). The mitochondrial marker enzymes citrate synthase (-34%), beta-hydroxyacyl-CoA dehydrogenase (-17%), and cytochrome c oxidase (-32%) were reduced (P < 0.05) in obese participants, indicating that mitochondrial content was diminished. Obesity did not alter the ability of isolated mitochondria to oxidize palmitate; however, fatty acid oxidation was reduced at the whole muscle level by 28% (P < 0.05) in the obese. Mitochondrial fatty acid translocase (FAT/CD36) did not differ in lean and obese individuals, but mitochondrial FAT/CD36 was correlated with mitochondrial fatty acid oxidation (r = 0.67, P < 0.05). We conclude that the reduction in fatty acid oxidation in obese individuals is attributable to a decrease in mitochondrial content, not to an intrinsic defect in the mitochondria obtained from skeletal muscle of obese individuals. In addition, it appears that mitochondrial FAT/CD36 may be involved in regulating fatty acid oxidation in human skeletal muscle.

Authors+Show Affiliations

Department of Human Health & Nutritional Sciences, University of Guelph, 50 Stone Rd., Guelph, Ontario, Canada N1G2W1. ghollowa@uoguelph.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17311893

Citation

Holloway, Graham P., et al. "Skeletal Muscle Mitochondrial FAT/CD36 Content and Palmitate Oxidation Are Not Decreased in Obese Women." American Journal of Physiology. Endocrinology and Metabolism, vol. 292, no. 6, 2007, pp. E1782-9.
Holloway GP, Thrush AB, Heigenhauser GJ, et al. Skeletal muscle mitochondrial FAT/CD36 content and palmitate oxidation are not decreased in obese women. Am J Physiol Endocrinol Metab. 2007;292(6):E1782-9.
Holloway, G. P., Thrush, A. B., Heigenhauser, G. J., Tandon, N. N., Dyck, D. J., Bonen, A., & Spriet, L. L. (2007). Skeletal muscle mitochondrial FAT/CD36 content and palmitate oxidation are not decreased in obese women. American Journal of Physiology. Endocrinology and Metabolism, 292(6), pp. E1782-9.
Holloway GP, et al. Skeletal Muscle Mitochondrial FAT/CD36 Content and Palmitate Oxidation Are Not Decreased in Obese Women. Am J Physiol Endocrinol Metab. 2007;292(6):E1782-9. PubMed PMID: 17311893.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Skeletal muscle mitochondrial FAT/CD36 content and palmitate oxidation are not decreased in obese women. AU - Holloway,Graham P, AU - Thrush,A Brianne, AU - Heigenhauser,George J F, AU - Tandon,Narendra N, AU - Dyck,David J, AU - Bonen,Arend, AU - Spriet,Lawrence L, Y1 - 2007/02/20/ PY - 2007/2/22/pubmed PY - 2007/7/31/medline PY - 2007/2/22/entrez SP - E1782 EP - 9 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 292 IS - 6 N2 - A reduction in fatty acid oxidation has been associated with lipid accumulation and insulin resistance in the skeletal muscle of obese individuals. We examined whether this decrease in fatty acid oxidation was attributable to a reduction in muscle mitochondrial content and/or a dysfunction in fatty acid oxidation within mitochondria obtained from skeletal muscle of age-matched, lean [body mass index (BMI) = 23.3 +/- 0.7 kg/m2] and obese women (BMI = 37.6 +/- 2.2 kg/m2). The mitochondrial marker enzymes citrate synthase (-34%), beta-hydroxyacyl-CoA dehydrogenase (-17%), and cytochrome c oxidase (-32%) were reduced (P < 0.05) in obese participants, indicating that mitochondrial content was diminished. Obesity did not alter the ability of isolated mitochondria to oxidize palmitate; however, fatty acid oxidation was reduced at the whole muscle level by 28% (P < 0.05) in the obese. Mitochondrial fatty acid translocase (FAT/CD36) did not differ in lean and obese individuals, but mitochondrial FAT/CD36 was correlated with mitochondrial fatty acid oxidation (r = 0.67, P < 0.05). We conclude that the reduction in fatty acid oxidation in obese individuals is attributable to a decrease in mitochondrial content, not to an intrinsic defect in the mitochondria obtained from skeletal muscle of obese individuals. In addition, it appears that mitochondrial FAT/CD36 may be involved in regulating fatty acid oxidation in human skeletal muscle. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/17311893/Skeletal_muscle_mitochondrial_FAT/CD36_content_and_palmitate_oxidation_are_not_decreased_in_obese_women_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00639.2006?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -