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[Effects of insulin on cardiac function and coronary circulation in acute myocardial ischemia and reperfusion experiment with dogs].
Zhonghua Yi Xue Za Zhi. 2006 Nov 28; 86(44):3128-32.ZY

Abstract

OBJECTIVE

To study the effect of insulin on cardiac functional recovery, coronary blood flow (CBF), coronary arterial function and coronary vascular endothelial cell apoptosis following acute myocardial ischemia/reperfusion (MI/R).

METHODS

In adult dogs, the left anterior descending coronary artery (LAD) was partially occluded (80% reduction in its blood flow) for 50 min and reperfused for 4 h. Vehicle (0.9% NaCl), GIK (glucose: 250 gxL(-1), insulin: 60 UxL(-1), potassium: 80 mmolxL(-1)), or GK (glucose: 250 gxL(-1), potassium: 80 mmolxL(-1)) were intravenously infused (2 mlxkg(-1)xh(-1)) 5 min before reperfusion, and CBF and left ventricular pressure were monitored. At the end of 4 h reperfusion period, coronary arteries were isolated, and the coronary vascular dysfunction, nitric oxide (NO) production and endothelial apoptosis were determined.

RESULTS

During reperfusion, compared with the vehicle, GIK increased CBFLAD (19.2 ml/min +/- 2.2 ml/min) vs (14.6 ml/min +/- 1.8 ml/min) of vehicle at the end of reperfusion, P < 0.05, improved recovery of LVSP and +/- LVdP/dtmax. In vivo ischemia/reperfusion caused significant coronary vascular endothelial dysfunction as evidenced by reduced endothelium dependent vasorelaxation, decreased total NO production, and endothelial cell apoptosis as determined by TUNEL staining. Reperfusion with GIK, but not GK, markedly improved the endothelium-dependent vasorelaxation (80.3% +/- 3.8%) vs. vehicle (28.1% +/- 2.3%, P < 0.01) of coronary artery in response to ACh. GIK significantly increased total NO production (17.19 micromol/L +/- 2.18 micromol/L) versus vehicle (4.74 micromol/L +/- 2.01 micromol/L, P < 0.01) and inhibited apoptosis in coronary arterial endothelial cell (12% +/- 4%) vs vehicle (45% +/- 7%, P < 0.01). GK failed to show any significant vasculoprotection against MI/R-induced coronary vascular injury.

CONCLUSION

These results demonstrate that insulin exerts cardioprotective effect by increasing CBF, reducing coronary artery injury and improving cardiac functional recovery during reperfusion, which may be partly attributable to the coronary vasculoprotective effect of insulin. The insulin-induced, NO-mediated anti-endothelial apoptotic effect may play a critical role in the insulin-induced coronary artery protective effect in MI/R.

Authors+Show Affiliations

Department of Physiology, Fourth Military Medical University, Xi'an 710032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17313765

Citation

Ma, Heng, et al. "[Effects of Insulin On Cardiac Function and Coronary Circulation in Acute Myocardial Ischemia and Reperfusion Experiment With Dogs]." Zhonghua Yi Xue Za Zhi, vol. 86, no. 44, 2006, pp. 3128-32.
Ma H, Huo JH, Zhang HX, et al. [Effects of insulin on cardiac function and coronary circulation in acute myocardial ischemia and reperfusion experiment with dogs]. Zhonghua Yi Xue Za Zhi. 2006;86(44):3128-32.
Ma, H., Huo, J. H., Zhang, H. X., Bian, J. F., Wang, Y. M., Xiong, L. Z., Guo, W. Y., Wang, H. C., & Gao, F. (2006). [Effects of insulin on cardiac function and coronary circulation in acute myocardial ischemia and reperfusion experiment with dogs]. Zhonghua Yi Xue Za Zhi, 86(44), 3128-32.
Ma H, et al. [Effects of Insulin On Cardiac Function and Coronary Circulation in Acute Myocardial Ischemia and Reperfusion Experiment With Dogs]. Zhonghua Yi Xue Za Zhi. 2006 Nov 28;86(44):3128-32. PubMed PMID: 17313765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of insulin on cardiac function and coronary circulation in acute myocardial ischemia and reperfusion experiment with dogs]. AU - Ma,Heng, AU - Huo,Jian-hua, AU - Zhang,Hang-xiang, AU - Bian,Jie-fang, AU - Wang,Yue-min, AU - Xiong,Li-ze, AU - Guo,Wen-yi, AU - Wang,Hai-chang, AU - Gao,Feng, PY - 2007/2/23/pubmed PY - 2008/4/25/medline PY - 2007/2/23/entrez SP - 3128 EP - 32 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 86 IS - 44 N2 - OBJECTIVE: To study the effect of insulin on cardiac functional recovery, coronary blood flow (CBF), coronary arterial function and coronary vascular endothelial cell apoptosis following acute myocardial ischemia/reperfusion (MI/R). METHODS: In adult dogs, the left anterior descending coronary artery (LAD) was partially occluded (80% reduction in its blood flow) for 50 min and reperfused for 4 h. Vehicle (0.9% NaCl), GIK (glucose: 250 gxL(-1), insulin: 60 UxL(-1), potassium: 80 mmolxL(-1)), or GK (glucose: 250 gxL(-1), potassium: 80 mmolxL(-1)) were intravenously infused (2 mlxkg(-1)xh(-1)) 5 min before reperfusion, and CBF and left ventricular pressure were monitored. At the end of 4 h reperfusion period, coronary arteries were isolated, and the coronary vascular dysfunction, nitric oxide (NO) production and endothelial apoptosis were determined. RESULTS: During reperfusion, compared with the vehicle, GIK increased CBFLAD (19.2 ml/min +/- 2.2 ml/min) vs (14.6 ml/min +/- 1.8 ml/min) of vehicle at the end of reperfusion, P < 0.05, improved recovery of LVSP and +/- LVdP/dtmax. In vivo ischemia/reperfusion caused significant coronary vascular endothelial dysfunction as evidenced by reduced endothelium dependent vasorelaxation, decreased total NO production, and endothelial cell apoptosis as determined by TUNEL staining. Reperfusion with GIK, but not GK, markedly improved the endothelium-dependent vasorelaxation (80.3% +/- 3.8%) vs. vehicle (28.1% +/- 2.3%, P < 0.01) of coronary artery in response to ACh. GIK significantly increased total NO production (17.19 micromol/L +/- 2.18 micromol/L) versus vehicle (4.74 micromol/L +/- 2.01 micromol/L, P < 0.01) and inhibited apoptosis in coronary arterial endothelial cell (12% +/- 4%) vs vehicle (45% +/- 7%, P < 0.01). GK failed to show any significant vasculoprotection against MI/R-induced coronary vascular injury. CONCLUSION: These results demonstrate that insulin exerts cardioprotective effect by increasing CBF, reducing coronary artery injury and improving cardiac functional recovery during reperfusion, which may be partly attributable to the coronary vasculoprotective effect of insulin. The insulin-induced, NO-mediated anti-endothelial apoptotic effect may play a critical role in the insulin-induced coronary artery protective effect in MI/R. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/17313765/[Effects_of_insulin_on_cardiac_function_and_coronary_circulation_in_acute_myocardial_ischemia_and_reperfusion_experiment_with_dogs]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2006&amp;vol=86&amp;issue=44&amp;fpage=3128 DB - PRIME DP - Unbound Medicine ER -