TY - JOUR T1 - [A follow up study of total IgM, IgG, nucleoprotein and spike protein antibodies against severe acute respiratory syndrome (SARS) coronavirus in patients with SARS]. AU - Yan,Hui-ping, AU - Tan,Yu-fen, AU - Zhuang,Hui, AU - Zhou,Yu-sen, AU - Zhao,Chun-hui, AU - Feng,Xia, AU - Jin,Rong-hua, AU - Wu,Hao, AU - Fu,Yan, PY - 2007/2/23/pubmed PY - 2007/6/23/medline PY - 2007/2/23/entrez SP - 896 EP - 9 JF - Zhonghua nei ke za zhi JO - Zhonghua Nei Ke Za Zhi VL - 45 IS - 11 N2 - OBJECTIVE: To survey the dynamic changing and persistence of the special antibodies, including total IgM, IgG, nucleocapsid protein and spike protein antibodies, against severe acute respiratory syndrome coronavirus (SARS-CoV) in patients with SARS. METHODS: 146 cases, all clinically diagnosed as SARS with positive SARS-CoV IgG, were followed up. 362 serum samples were collected from the onset of the disease to 660 days afterward. Total IgM and IgG against SARS-CoV were tested with commercial ELISA kits. For recombinant nucleoprotein and spike protein, we developed an ELISA to test these two antibodies. RESULTS: Within 20 days of the onset, the positive rate of anti-SARS-CoV IgM was 46.5% (20/43); it reached a peak after 21 - 40 days (80.6%, 25/31). Then, the positive rate of IgM went down gradually to 8.2% (6/73) until 550 days after the onset. The patient's IgG positive rate was lower (34.9%, 15/43) than that of IgM within 20 days of the onset. Then it went up rapidly to 100%. It remained positive (98.6%, 70/71) until 600 - 660 days after the onset. When N-IgG and S-IgG were tested 40 days after the onset of the disease at three different times, the positive rate of N-IgG (92.5%, 37/40) was higher than that of S-IgG (67.5%, 27/40), but the two structure protein antibodies were always lower than the total IgG. CONCLUSIONS: In SARS patients with definite clinical and etiological diagnosis, the highest positive rate of the antibodies against SARS-CoV was found at 21 - 40 days after the onset. IgM disappeared almost 500 days (91.8%) after the onset. Total IgG positive rate could reach 100% and 98.6% and the positivity might persist nearly two years. So it is speculated that the total IgG antibody may be positive 3 to 5 years after infection, but it seems that N-IgG and S-IgG keep positive shorter in time than total IgG antibody. SN - 0578-1426 UR - https://www.unboundmedicine.com/medline/citation/17313874/[A_follow_up_study_of_total_IgM_IgG_nucleoprotein_and_spike_protein_antibodies_against_severe_acute_respiratory_syndrome__SARS__coronavirus_in_patients_with_SARS]_ DB - PRIME DP - Unbound Medicine ER -