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On-line concentration and determination of all-trans- and 13-cis- retinoic acids in rabbit serum by application of sweeping technique in micellar electrokinetic chromatography.
J Chromatogr A. 2007 Mar 30; 1146(1):131-5.JC

Abstract

A simple and rapid micellar electrokinetic chromatography (MEKC) method with UV detection was developed for the simultaneous separation and determination of all-trans- and 13-cis-retinoic acids in rabbit serum by on-line sweeping concentration technique. The serum sample was simply deproteinized and centrifuged. Various parameters affecting sample enrichment and separation were systematically investigated. Under optimal conditions, the analytes could be well separated within 17min, and the relative standard deviations (RSD) of migration times and peak areas were less than 3.4%. Compared with the conventional MEKC injection method, the 18- and 19-fold improvements in sensitivity were achieved, respectively. The proposed method has been successfully applied to the determination of all-trans- and 13-cis-retinoic acids in serum samples from rabbits and could be feasible for the further pharmacokinetics study of all-trans-retinoic acid.

Authors+Show Affiliations

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. vzhaoyx@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17316656

Citation

Zhao, Yongxi, et al. "On-line Concentration and Determination of All-trans- and 13-cis- Retinoic Acids in Rabbit Serum By Application of Sweeping Technique in Micellar Electrokinetic Chromatography." Journal of Chromatography. A, vol. 1146, no. 1, 2007, pp. 131-5.
Zhao Y, Kong Y, Wang B, et al. On-line concentration and determination of all-trans- and 13-cis- retinoic acids in rabbit serum by application of sweeping technique in micellar electrokinetic chromatography. J Chromatogr A. 2007;1146(1):131-5.
Zhao, Y., Kong, Y., Wang, B., Wu, Y., & Wu, H. (2007). On-line concentration and determination of all-trans- and 13-cis- retinoic acids in rabbit serum by application of sweeping technique in micellar electrokinetic chromatography. Journal of Chromatography. A, 1146(1), 131-5.
Zhao Y, et al. On-line Concentration and Determination of All-trans- and 13-cis- Retinoic Acids in Rabbit Serum By Application of Sweeping Technique in Micellar Electrokinetic Chromatography. J Chromatogr A. 2007 Mar 30;1146(1):131-5. PubMed PMID: 17316656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line concentration and determination of all-trans- and 13-cis- retinoic acids in rabbit serum by application of sweeping technique in micellar electrokinetic chromatography. AU - Zhao,Yongxi, AU - Kong,Yu, AU - Wang,Bo, AU - Wu,Yayan, AU - Wu,Hong, Y1 - 2007/02/11/ PY - 2006/12/26/received PY - 2007/01/31/revised PY - 2007/02/06/accepted PY - 2007/2/24/pubmed PY - 2007/5/1/medline PY - 2007/2/24/entrez SP - 131 EP - 5 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1146 IS - 1 N2 - A simple and rapid micellar electrokinetic chromatography (MEKC) method with UV detection was developed for the simultaneous separation and determination of all-trans- and 13-cis-retinoic acids in rabbit serum by on-line sweeping concentration technique. The serum sample was simply deproteinized and centrifuged. Various parameters affecting sample enrichment and separation were systematically investigated. Under optimal conditions, the analytes could be well separated within 17min, and the relative standard deviations (RSD) of migration times and peak areas were less than 3.4%. Compared with the conventional MEKC injection method, the 18- and 19-fold improvements in sensitivity were achieved, respectively. The proposed method has been successfully applied to the determination of all-trans- and 13-cis-retinoic acids in serum samples from rabbits and could be feasible for the further pharmacokinetics study of all-trans-retinoic acid. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17316656/On_line_concentration_and_determination_of_all_trans__and_13_cis__retinoic_acids_in_rabbit_serum_by_application_of_sweeping_technique_in_micellar_electrokinetic_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00279-8 DB - PRIME DP - Unbound Medicine ER -