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New high-performance liquid chromatographic method for sensitive determination of pheomelanin in biological materials by precolumn fluorescence derivatization with naphthalene-2,3-dicarboxaldehyde.
J Chromatogr A. 2007 Mar 30; 1146(1):23-31.JC

Abstract

Pheomelanin is an important type of melanin distributed in the skin, eye and hair in the mammal, which is of great social, clinical and cosmetic significance. In this study, a new HPLC method with fluorescence detection is described originally for the sensitive determination of pheomelanin in biological materials. The pheomelanin polymer is decomposed into two specific degradation products, 3-amino-4-hydroxyphenylalanine (3-AHP) and 4-amino-3- hydroxyphenylalanine (4-AHP) with hydriodic acid. Then the two AHP isomers are derivatized using a fluorescent probe naphthalene-2,3-dicarboxaldehyde in the presence of cyanide. The resulting highly stable 2-substituted 1-cyanobenz[f] isoindole derivatives were separated on a 5NH(2)-MS aminopropyl packed HPLC column with binary isocratic elution profile and detected fluorimetrically. The assay shows high sensitivity of 0.11nM (2.2fmol per injection, the lowest reported) at signal-to-noise ratio of 3 for each AHPs, good accuracy and precision (RSDs<3.1%), and linearity (range of 0.02-10microM, r>0.995). The results obtained by using fluorescence detection have been compared with other detection systems (electrochemical and UV). The sensitivity can increase from 100 to respect electrochemical detection and 30000 times respect UV detection. The method has been used for the quantitative determination of pheomelanin in various biological samples, including cell cultures from five types of melanoma cell lines of human and rat origin, hair samples of various colors, melanoma tissue and the urines from human melanoma patients and healthy subjects. This original application of HPLC-fluorescence detection represents a powerful tool for investigating pheomelanin synthesis in vitro and in vivo under physiological and pathophysiological conditions.

Authors+Show Affiliations

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17316662

Citation

Yang, Qiao, et al. "New High-performance Liquid Chromatographic Method for Sensitive Determination of Pheomelanin in Biological Materials By Precolumn Fluorescence Derivatization With Naphthalene-2,3-dicarboxaldehyde." Journal of Chromatography. A, vol. 1146, no. 1, 2007, pp. 23-31.
Yang Q, Zhang XL, Ma M, et al. New high-performance liquid chromatographic method for sensitive determination of pheomelanin in biological materials by precolumn fluorescence derivatization with naphthalene-2,3-dicarboxaldehyde. J Chromatogr A. 2007;1146(1):23-31.
Yang, Q., Zhang, X. L., Ma, M., Huang, K. J., Zhang, J. X., Ni, W. Z., Fang, C. X., & Zheng, C. Y. (2007). New high-performance liquid chromatographic method for sensitive determination of pheomelanin in biological materials by precolumn fluorescence derivatization with naphthalene-2,3-dicarboxaldehyde. Journal of Chromatography. A, 1146(1), 23-31.
Yang Q, et al. New High-performance Liquid Chromatographic Method for Sensitive Determination of Pheomelanin in Biological Materials By Precolumn Fluorescence Derivatization With Naphthalene-2,3-dicarboxaldehyde. J Chromatogr A. 2007 Mar 30;1146(1):23-31. PubMed PMID: 17316662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New high-performance liquid chromatographic method for sensitive determination of pheomelanin in biological materials by precolumn fluorescence derivatization with naphthalene-2,3-dicarboxaldehyde. AU - Yang,Qiao, AU - Zhang,Xiao-Ling, AU - Ma,Ming, AU - Huang,Ke-Jing, AU - Zhang,Jun-Xiang, AU - Ni,Wu-Zhong, AU - Fang,Cheng-Xiang, AU - Zheng,Cong-Yi, Y1 - 2007/01/28/ PY - 2006/12/03/received PY - 2007/01/17/revised PY - 2007/01/22/accepted PY - 2007/2/24/pubmed PY - 2007/5/1/medline PY - 2007/2/24/entrez SP - 23 EP - 31 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1146 IS - 1 N2 - Pheomelanin is an important type of melanin distributed in the skin, eye and hair in the mammal, which is of great social, clinical and cosmetic significance. In this study, a new HPLC method with fluorescence detection is described originally for the sensitive determination of pheomelanin in biological materials. The pheomelanin polymer is decomposed into two specific degradation products, 3-amino-4-hydroxyphenylalanine (3-AHP) and 4-amino-3- hydroxyphenylalanine (4-AHP) with hydriodic acid. Then the two AHP isomers are derivatized using a fluorescent probe naphthalene-2,3-dicarboxaldehyde in the presence of cyanide. The resulting highly stable 2-substituted 1-cyanobenz[f] isoindole derivatives were separated on a 5NH(2)-MS aminopropyl packed HPLC column with binary isocratic elution profile and detected fluorimetrically. The assay shows high sensitivity of 0.11nM (2.2fmol per injection, the lowest reported) at signal-to-noise ratio of 3 for each AHPs, good accuracy and precision (RSDs<3.1%), and linearity (range of 0.02-10microM, r>0.995). The results obtained by using fluorescence detection have been compared with other detection systems (electrochemical and UV). The sensitivity can increase from 100 to respect electrochemical detection and 30000 times respect UV detection. The method has been used for the quantitative determination of pheomelanin in various biological samples, including cell cultures from five types of melanoma cell lines of human and rat origin, hair samples of various colors, melanoma tissue and the urines from human melanoma patients and healthy subjects. This original application of HPLC-fluorescence detection represents a powerful tool for investigating pheomelanin synthesis in vitro and in vivo under physiological and pathophysiological conditions. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17316662/New_high_performance_liquid_chromatographic_method_for_sensitive_determination_of_pheomelanin_in_biological_materials_by_precolumn_fluorescence_derivatization_with_naphthalene_23_dicarboxaldehyde_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00152-5 DB - PRIME DP - Unbound Medicine ER -