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Determination of biogenic amines in alcoholic beverages by ion chromatography with suppressed conductivity detection and integrated pulsed amperometric detection.
J Chromatogr A. 2007 Jun 29; 1155(1):22-30.JC

Abstract

The determination of biogenic amines in alcoholic beverages is important to assess the potential risks associated with the consumption of high concentrations of these compounds. In addition, product storage conditions and the length of storage can cause the formation of biogenic amines that reduce product quality. We report a new method using cation-exchange chromatography with either suppressed conductivity, integrated pulsed amperometry, UV, or a combination of these detection techniques to determine biogenic amines in alcoholic beverages. The main objective was to provide a direct comparison between IPAD and suppressed conductivity detection for determining biogenic amines in alcoholic beverages. Suppressed conductivity is the simplest detection approach for determining putrescine, cadaverine, histamine, agmatine, phenylethylamine, spermidine, and spermine with good sensitivity (0.004-0.08 mg/l) and was used to evaluate the influence of storage time and conditions on the evolution of biogenic amines in alcoholic beverages. Integrated pulsed amperometric detection (IPAD) detects more biogenic amines than suppressed conductivity detection, enabling the detection of dopamine, tyramine, and serotonin. Tyramine was simultaneously determined by UV detection and IPAD to provide confirmation and ensure the accuracy of the analytical results. The linearity of biogenic amine responses was within 0.1-20 mg/l and peak area precisions were 0.24-4.97% for IPAD, suppressed conductivity-IPAD, and UV detection. The sensitivity for the 10 biogenic amines using the 3 detection techniques varied considerably from 0.004-1.1 mg/l and recoveries were within 85-122%.

Authors+Show Affiliations

Dionex Corporation, Sunnyvale, CA 94088-3606, USA. brian.deborba@dionex.comNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17320892

Citation

De Borba, Brian M., and Jeff S. Rohrer. "Determination of Biogenic Amines in Alcoholic Beverages By Ion Chromatography With Suppressed Conductivity Detection and Integrated Pulsed Amperometric Detection." Journal of Chromatography. A, vol. 1155, no. 1, 2007, pp. 22-30.
De Borba BM, Rohrer JS. Determination of biogenic amines in alcoholic beverages by ion chromatography with suppressed conductivity detection and integrated pulsed amperometric detection. J Chromatogr A. 2007;1155(1):22-30.
De Borba, B. M., & Rohrer, J. S. (2007). Determination of biogenic amines in alcoholic beverages by ion chromatography with suppressed conductivity detection and integrated pulsed amperometric detection. Journal of Chromatography. A, 1155(1), 22-30.
De Borba BM, Rohrer JS. Determination of Biogenic Amines in Alcoholic Beverages By Ion Chromatography With Suppressed Conductivity Detection and Integrated Pulsed Amperometric Detection. J Chromatogr A. 2007 Jun 29;1155(1):22-30. PubMed PMID: 17320892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of biogenic amines in alcoholic beverages by ion chromatography with suppressed conductivity detection and integrated pulsed amperometric detection. AU - De Borba,Brian M, AU - Rohrer,Jeff S, Y1 - 2007/02/04/ PY - 2006/10/25/received PY - 2007/01/16/revised PY - 2007/01/25/accepted PY - 2007/2/27/pubmed PY - 2007/8/19/medline PY - 2007/2/27/entrez SP - 22 EP - 30 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1155 IS - 1 N2 - The determination of biogenic amines in alcoholic beverages is important to assess the potential risks associated with the consumption of high concentrations of these compounds. In addition, product storage conditions and the length of storage can cause the formation of biogenic amines that reduce product quality. We report a new method using cation-exchange chromatography with either suppressed conductivity, integrated pulsed amperometry, UV, or a combination of these detection techniques to determine biogenic amines in alcoholic beverages. The main objective was to provide a direct comparison between IPAD and suppressed conductivity detection for determining biogenic amines in alcoholic beverages. Suppressed conductivity is the simplest detection approach for determining putrescine, cadaverine, histamine, agmatine, phenylethylamine, spermidine, and spermine with good sensitivity (0.004-0.08 mg/l) and was used to evaluate the influence of storage time and conditions on the evolution of biogenic amines in alcoholic beverages. Integrated pulsed amperometric detection (IPAD) detects more biogenic amines than suppressed conductivity detection, enabling the detection of dopamine, tyramine, and serotonin. Tyramine was simultaneously determined by UV detection and IPAD to provide confirmation and ensure the accuracy of the analytical results. The linearity of biogenic amine responses was within 0.1-20 mg/l and peak area precisions were 0.24-4.97% for IPAD, suppressed conductivity-IPAD, and UV detection. The sensitivity for the 10 biogenic amines using the 3 detection techniques varied considerably from 0.004-1.1 mg/l and recoveries were within 85-122%. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17320892/Determination_of_biogenic_amines_in_alcoholic_beverages_by_ion_chromatography_with_suppressed_conductivity_detection_and_integrated_pulsed_amperometric_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00220-8 DB - PRIME DP - Unbound Medicine ER -