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Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen-presenting cells that induce proallergic T cells.
J Allergy Clin Immunol. 2007 Apr; 119(4):982-90.JA

Abstract

BACKGROUND

Thymic stromal lymphopoietin (TSLP) endows human CD11c(+) dendritic cells (DCs) from peripheral blood with the capacity to induce proallergic T cells. TSLP is present at high levels in the epidermis of atopic dermatitis where it appears to trigger emigration of epidermal Langerhans cells (LCs); however, nothing else is known about the influence of TSLP on LCs.

OBJECTIVE

Effects of TSLP on human epidermal LCs were investigated.

METHODS

LCs were isolated by trypsinization from healthy human skin, highly enriched by immunomagnetic techniques (via CD1a) and cultured for 2 days. Additionally, migratory LCs were obtained by emigration from epidermal sheets for 3 days.

RESULTS

The addition of TSLP promoted survival and maturation of LCs obtained by trypsinization, as indicated by their increased expression of CD83, CD86, and high levels of MHC II. TSLP markedly increased numbers of migratory LCs. Allogeneic naïve CD4(+) T cells, cocultured with migratory TSLP-LCs produced less IFN-gamma and IL-10 and more IL-4, IL-5, IL-13, and TNF-alpha. Finally, TSLP-LCs secreted markedly more of the T(H)2 T-cell-attracting chemokine CCL17/thymus and activation-regulated chemokine.

CONCLUSION

These cytokine patterns correspond to those described for TSLP-treated blood DCs. They highlight a direct effect of TSLP on epidermal LCs.

CLINICAL IMPLICATIONS

Our data emphasize a critical role for LCs in the triggering of atopic dermatitis. Furthermore, they underscore the interest in TSLP as a potential therapeutic target in atopic diseases.

Authors+Show Affiliations

Department of Dermatology and Venereology, Kompetenzzentrum Medizin Tirol, Innsbruck, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17320941

Citation

Ebner, Susanne, et al. "Thymic Stromal Lymphopoietin Converts Human Epidermal Langerhans Cells Into Antigen-presenting Cells That Induce Proallergic T Cells." The Journal of Allergy and Clinical Immunology, vol. 119, no. 4, 2007, pp. 982-90.
Ebner S, Nguyen VA, Forstner M, et al. Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen-presenting cells that induce proallergic T cells. J Allergy Clin Immunol. 2007;119(4):982-90.
Ebner, S., Nguyen, V. A., Forstner, M., Wang, Y. H., Wolfram, D., Liu, Y. J., & Romani, N. (2007). Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen-presenting cells that induce proallergic T cells. The Journal of Allergy and Clinical Immunology, 119(4), 982-90.
Ebner S, et al. Thymic Stromal Lymphopoietin Converts Human Epidermal Langerhans Cells Into Antigen-presenting Cells That Induce Proallergic T Cells. J Allergy Clin Immunol. 2007;119(4):982-90. PubMed PMID: 17320941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen-presenting cells that induce proallergic T cells. AU - Ebner,Susanne, AU - Nguyen,Van Anh, AU - Forstner,Markus, AU - Wang,Yui-Hsi, AU - Wolfram,Dolores, AU - Liu,Yong-Jun, AU - Romani,Nikolaus, Y1 - 2007/02/22/ PY - 2006/04/02/received PY - 2006/12/11/revised PY - 2007/01/02/accepted PY - 2007/2/27/pubmed PY - 2007/5/23/medline PY - 2007/2/27/entrez SP - 982 EP - 90 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 119 IS - 4 N2 - BACKGROUND: Thymic stromal lymphopoietin (TSLP) endows human CD11c(+) dendritic cells (DCs) from peripheral blood with the capacity to induce proallergic T cells. TSLP is present at high levels in the epidermis of atopic dermatitis where it appears to trigger emigration of epidermal Langerhans cells (LCs); however, nothing else is known about the influence of TSLP on LCs. OBJECTIVE: Effects of TSLP on human epidermal LCs were investigated. METHODS: LCs were isolated by trypsinization from healthy human skin, highly enriched by immunomagnetic techniques (via CD1a) and cultured for 2 days. Additionally, migratory LCs were obtained by emigration from epidermal sheets for 3 days. RESULTS: The addition of TSLP promoted survival and maturation of LCs obtained by trypsinization, as indicated by their increased expression of CD83, CD86, and high levels of MHC II. TSLP markedly increased numbers of migratory LCs. Allogeneic naïve CD4(+) T cells, cocultured with migratory TSLP-LCs produced less IFN-gamma and IL-10 and more IL-4, IL-5, IL-13, and TNF-alpha. Finally, TSLP-LCs secreted markedly more of the T(H)2 T-cell-attracting chemokine CCL17/thymus and activation-regulated chemokine. CONCLUSION: These cytokine patterns correspond to those described for TSLP-treated blood DCs. They highlight a direct effect of TSLP on epidermal LCs. CLINICAL IMPLICATIONS: Our data emphasize a critical role for LCs in the triggering of atopic dermatitis. Furthermore, they underscore the interest in TSLP as a potential therapeutic target in atopic diseases. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/17320941/Thymic_stromal_lymphopoietin_converts_human_epidermal_Langerhans_cells_into_antigen_presenting_cells_that_induce_proallergic_T_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(07)00118-2 DB - PRIME DP - Unbound Medicine ER -