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Development of a new neutralization test for measles virus.
J Virol Methods. 2007 Jun; 142(1-2):15-20.JV

Abstract

Sero-epidemiological studies are required to identify populations susceptible to measles. The hemagglutination inhibition (HI) test is no longer sensitive enough to confirm immunity to measles, and at present the particle agglutination (PA) test and enzyme-linked immunosorbent assay (EIA) are employed. The most reliable method is the neutralization test (NT), but it is time-consuming and requires experience. To simplify the NT, a recombinant measles AIK-C virus expressing green fluorescence protein (GFP-MVAIK) was constructed and used as a challenge virus. Plaques and cytopathic effects were visualized under ultraviolet light and detected easily, and measuring the intensity of the fluorescence enabled a reduction in the time-consuming steps. Neutralizing antibody titers of a complete inhibition neutralization test were equivalent to those of a 90% plaque reduction neutralization test. Comparison of four methods, HI, PA, EIA and the complete inhibition neutralization test, showed that only the results of EIA correlated well with those of the complete inhibition neutralization test, but sera with borderline levels by EIA were sometimes negative by the complete inhibition neutralization assay.

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Authors+Show Affiliations

Fujino M
Kitasato Institutes for Life Sciences, Laboratory of Viral Infection, Shirokane 5-9-1, Minato-ku, Tokyo 108-8641, Japan. motokof@lisci.kitasato-u.ac.jp
Yoshida N
No affiliation info available
Kimura K
No affiliation info available
Zhou J
No affiliation info available
Motegi Y
No affiliation info available
Komase K
No affiliation info available
Nakayama T
No affiliation info available

MeSH

Agglutination TestsAnimalsAntibodies, ViralAntigens, ViralCell LineChildChlorocebus aethiopsCytopathogenic Effect, ViralEnzyme-Linked Immunosorbent AssayGreen Fluorescent ProteinsHemagglutination Inhibition TestsHumansMeaslesMeasles virusNeutralization TestsRecombinant ProteinsVero CellsViral Plaque Assay

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17320979

Citation

Fujino, Motoko, et al. "Development of a New Neutralization Test for Measles Virus." Journal of Virological Methods, vol. 142, no. 1-2, 2007, pp. 15-20.
Fujino M, Yoshida N, Kimura K, et al. Development of a new neutralization test for measles virus. J Virol Methods. 2007;142(1-2):15-20.
Fujino, M., Yoshida, N., Kimura, K., Zhou, J., Motegi, Y., Komase, K., & Nakayama, T. (2007). Development of a new neutralization test for measles virus. Journal of Virological Methods, 142(1-2), 15-20.
Fujino M, et al. Development of a New Neutralization Test for Measles Virus. J Virol Methods. 2007;142(1-2):15-20. PubMed PMID: 17320979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a new neutralization test for measles virus. AU - Fujino,Motoko, AU - Yoshida,Naoko, AU - Kimura,Keiko, AU - Zhou,Jianhui, AU - Motegi,Yoshie, AU - Komase,Katsuhiro, AU - Nakayama,Tetsuo, Y1 - 2007/02/23/ PY - 2005/09/15/received PY - 2006/12/06/revised PY - 2007/01/11/accepted PY - 2007/2/27/pubmed PY - 2007/6/22/medline PY - 2007/2/27/entrez SP - 15 EP - 20 JF - Journal of virological methods JO - J Virol Methods VL - 142 IS - 1-2 N2 - Sero-epidemiological studies are required to identify populations susceptible to measles. The hemagglutination inhibition (HI) test is no longer sensitive enough to confirm immunity to measles, and at present the particle agglutination (PA) test and enzyme-linked immunosorbent assay (EIA) are employed. The most reliable method is the neutralization test (NT), but it is time-consuming and requires experience. To simplify the NT, a recombinant measles AIK-C virus expressing green fluorescence protein (GFP-MVAIK) was constructed and used as a challenge virus. Plaques and cytopathic effects were visualized under ultraviolet light and detected easily, and measuring the intensity of the fluorescence enabled a reduction in the time-consuming steps. Neutralizing antibody titers of a complete inhibition neutralization test were equivalent to those of a 90% plaque reduction neutralization test. Comparison of four methods, HI, PA, EIA and the complete inhibition neutralization test, showed that only the results of EIA correlated well with those of the complete inhibition neutralization test, but sera with borderline levels by EIA were sometimes negative by the complete inhibition neutralization assay. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/17320979/Development_of_a_new_neutralization_test_for_measles_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(07)00013-4 DB - PRIME DP - Unbound Medicine ER -