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Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural salinity gradient of the Baltic Sea.
Aquat Toxicol. 2007 Apr 20; 82(1):63-71.AT

Abstract

Blue mussels (Mytilus edulis L.) collected at three sampling sites in each of three geographical regions (South, Middle, North) along the permanent longitudinal South-North salinity gradient of the Baltic Sea, were exposed for 10 days to copper (35ppb) or 95 octane petrol (0.3 per thousand). During the experiment, they were maintained at the respective sampling site salinity. Scope for growth (SFG) was determined, and biochemical stress markers (protein carbonyl groups, disulfide bond formation, and glutathione transferase (GST), and catalase (CAT) activities) were investigated in gill tissue upon termination of the experiment. Treatment and regional effects for SFG and protein carbonyl groups were all significant for petrol. The largest increase in protein carbonyl groups was observed in the North. Mussels from the southern, more saline (approximately 7 per thousand) region had the highest SFG, and displayed the largest SFG decrease in response to treatment, indicating that they had the most energy available for allocation to stress response. They also displayed the least increase in the level of protein carbonyl groups. Mussels from the Northern, less saline (approximately 5%) region had the highest degree of protein carbonyl groups in response to both treatments, and lowest average SFG. Silver stained diagonal gels for samples from one sampling site in South and North, respectively, demonstrated differences in disulfide bond profiles for both stress treatments. There was also a regional difference in the number of protein disulfides observed on diagonal gels. The most diverse protein disulfide response was found in South. No treatment related effects on GST and CAT activities were observed. We suggest that both SFG and protein carbonyl groups show that geographical difference in stress susceptibility, previously established between the North and the Baltic Seas, also apply on a regional scale within the Baltic Sea, along the salinity gradient.

Authors+Show Affiliations

School of Life Sciences, Södertörn University College, S-141 89 Huddinge, Sweden. andreas.prevodnik@sh.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17320983

Citation

Prevodnik, Andreas, et al. "Oxidative Stress in Response to Xenobiotics in the Blue Mussel Mytilus Edulis L.: Evidence for Variation Along a Natural Salinity Gradient of the Baltic Sea." Aquatic Toxicology (Amsterdam, Netherlands), vol. 82, no. 1, 2007, pp. 63-71.
Prevodnik A, Gardeström J, Lilja K, et al. Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural salinity gradient of the Baltic Sea. Aquat Toxicol. 2007;82(1):63-71.
Prevodnik, A., Gardeström, J., Lilja, K., Elfwing, T., McDonagh, B., Petrović, N., Tedengren, M., Sheehan, D., & Bollner, T. (2007). Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural salinity gradient of the Baltic Sea. Aquatic Toxicology (Amsterdam, Netherlands), 82(1), 63-71.
Prevodnik A, et al. Oxidative Stress in Response to Xenobiotics in the Blue Mussel Mytilus Edulis L.: Evidence for Variation Along a Natural Salinity Gradient of the Baltic Sea. Aquat Toxicol. 2007 Apr 20;82(1):63-71. PubMed PMID: 17320983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural salinity gradient of the Baltic Sea. AU - Prevodnik,Andreas, AU - Gardeström,Johanna, AU - Lilja,Karl, AU - Elfwing,Tina, AU - McDonagh,Brian, AU - Petrović,Natasa, AU - Tedengren,Michael, AU - Sheehan,David, AU - Bollner,Tomas, Y1 - 2007/01/30/ PY - 2006/12/21/received PY - 2007/01/22/revised PY - 2007/01/23/accepted PY - 2007/2/27/pubmed PY - 2007/5/26/medline PY - 2007/2/27/entrez SP - 63 EP - 71 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 82 IS - 1 N2 - Blue mussels (Mytilus edulis L.) collected at three sampling sites in each of three geographical regions (South, Middle, North) along the permanent longitudinal South-North salinity gradient of the Baltic Sea, were exposed for 10 days to copper (35ppb) or 95 octane petrol (0.3 per thousand). During the experiment, they were maintained at the respective sampling site salinity. Scope for growth (SFG) was determined, and biochemical stress markers (protein carbonyl groups, disulfide bond formation, and glutathione transferase (GST), and catalase (CAT) activities) were investigated in gill tissue upon termination of the experiment. Treatment and regional effects for SFG and protein carbonyl groups were all significant for petrol. The largest increase in protein carbonyl groups was observed in the North. Mussels from the southern, more saline (approximately 7 per thousand) region had the highest SFG, and displayed the largest SFG decrease in response to treatment, indicating that they had the most energy available for allocation to stress response. They also displayed the least increase in the level of protein carbonyl groups. Mussels from the Northern, less saline (approximately 5%) region had the highest degree of protein carbonyl groups in response to both treatments, and lowest average SFG. Silver stained diagonal gels for samples from one sampling site in South and North, respectively, demonstrated differences in disulfide bond profiles for both stress treatments. There was also a regional difference in the number of protein disulfides observed on diagonal gels. The most diverse protein disulfide response was found in South. No treatment related effects on GST and CAT activities were observed. We suggest that both SFG and protein carbonyl groups show that geographical difference in stress susceptibility, previously established between the North and the Baltic Seas, also apply on a regional scale within the Baltic Sea, along the salinity gradient. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/17320983/Oxidative_stress_in_response_to_xenobiotics_in_the_blue_mussel_Mytilus_edulis_L_:_evidence_for_variation_along_a_natural_salinity_gradient_of_the_Baltic_Sea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(07)00040-9 DB - PRIME DP - Unbound Medicine ER -