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[The role of alveolar macrophages in airway remodeling in asthmatic rats].
Zhonghua Jie He He Hu Xi Za Zhi. 2006 Nov; 29(11):740-3.ZJ

Abstract

OBJECTIVE

To study the role of alveolar macrophages (AM) in the processes of airway remodeling in asthmatic rats.

METHODS

Forty-eight young male Sprague-Dawley rats (Grade II) were divided randomly into a control group (A group), a 3 day asthma group (B group), a 14 day asthma group (C group) and a 30 day asthma group (D group). The rats were sensitized and challenged by ovalbumin to establish the asthmatic model. AM were purified from bronchoalveolar lavage. The content of tumor necrosis factor-alpha (TNF-alpha) in AM was measured by enzyme-linked immunosorbent assay, prostaglandin E2 (PGE2) by radioimmunoassay, matrix metalloproteinase-9 mRNA (MMP-9 mRNA) and tissue inhibitor of metalloproteinase-1 mRNA (TIMP-1 mRNA) by hybridization in situ. The total bronchial wall area (WAt) and the smooth muscle area (WAm) were measured by image analysis system. The WAt and the WAm were quantified per unit length of basement membrane (Pbm).

RESULTS

The bronchial wall thickness and the smooth muscle thickness of D group [(85 +/- 9) microm2/microm, (28.6 +/- 4.9) microm2/microm] were significantly higher than those of A group [(67 +/- 10) microm2/microm, (16.8 +/- 2.4) microm2/microm, t = 2.938, 3.227, all P < 0.01]. The contents of TNF-alpha and PGE2 in D group [(0.68 +/- 0.25) microg/L, (0.122 +/- 0.030) microg/L] were significantly higher than those in A group [(0.37 +/- 0.09) microg/L, (0.079 +/- 0.018) microg/L, t = 2.683, 3.016, all P < 0.01]. When A group was compared with B group [(0.74 +/- 0.29) microg/L, (0.120 +/- 0.028) microg/L] and C group [(0.71 +/- 0.23) microg/L, (0.117 +/- 0.028) microg/L], there was no significant difference (t = 1.624, 0.472, all P > 0.05) and (t = 0.935, 0.533, all P > 0.05). The contents (light density) of MMP-9 mRNA and TIMP-1 mRNA in D group (0.346 +/- 0.033, 0.361 +/- 0.040) were significantly higher than C group (0.279 +/- 0.015, 0.259 +/- 0.015, t = 2.574, 2.716, all P < 0.01), and so did D group and B group (0.183 +/- 0.025, 0.136 +/- 0.014, t = 2.913, 3.017, all P < 0.01), D group and A group (0.104 +/- 0.007, 0.109 +/- 0.008, t = 3.632, 3.487, all P < 0.01). There were significant correlations between the contents of MMP-9 mRNA and WAt (r = 0.693, P < 0.01), between MMP-9 mRNA and WAm (r = 0.738, P < 0.01), between TIMP-1 mRNA and WAt (r = 0.823, P < 0.01), and between TIMP-1 mRNA and WAm (r = 0.876, P < 0.01).

CONCLUSION

AM and AM-derived cytokines are associated with airway remodeling in asthmatic rats.

Authors+Show Affiliations

Affiliated Yuying Children's Hospital, Wenzhou Medical College, Wenzhou 325027, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17327053

Citation

Wang, Chuan-xia, et al. "[The Role of Alveolar Macrophages in Airway Remodeling in Asthmatic Rats]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 29, no. 11, 2006, pp. 740-3.
Wang CX, Li CC, Luo YC, et al. [The role of alveolar macrophages in airway remodeling in asthmatic rats]. Zhonghua Jie He He Hu Xi Za Zhi. 2006;29(11):740-3.
Wang, C. X., Li, C. C., Luo, Y. C., & Li, M. R. (2006). [The role of alveolar macrophages in airway remodeling in asthmatic rats]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 29(11), 740-3.
Wang CX, et al. [The Role of Alveolar Macrophages in Airway Remodeling in Asthmatic Rats]. Zhonghua Jie He He Hu Xi Za Zhi. 2006;29(11):740-3. PubMed PMID: 17327053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The role of alveolar macrophages in airway remodeling in asthmatic rats]. AU - Wang,Chuan-xia, AU - Li,Chang-chong, AU - Luo,Yun-chun, AU - Li,Meng-rong, PY - 2007/3/1/pubmed PY - 2010/9/8/medline PY - 2007/3/1/entrez SP - 740 EP - 3 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 29 IS - 11 N2 - OBJECTIVE: To study the role of alveolar macrophages (AM) in the processes of airway remodeling in asthmatic rats. METHODS: Forty-eight young male Sprague-Dawley rats (Grade II) were divided randomly into a control group (A group), a 3 day asthma group (B group), a 14 day asthma group (C group) and a 30 day asthma group (D group). The rats were sensitized and challenged by ovalbumin to establish the asthmatic model. AM were purified from bronchoalveolar lavage. The content of tumor necrosis factor-alpha (TNF-alpha) in AM was measured by enzyme-linked immunosorbent assay, prostaglandin E2 (PGE2) by radioimmunoassay, matrix metalloproteinase-9 mRNA (MMP-9 mRNA) and tissue inhibitor of metalloproteinase-1 mRNA (TIMP-1 mRNA) by hybridization in situ. The total bronchial wall area (WAt) and the smooth muscle area (WAm) were measured by image analysis system. The WAt and the WAm were quantified per unit length of basement membrane (Pbm). RESULTS: The bronchial wall thickness and the smooth muscle thickness of D group [(85 +/- 9) microm2/microm, (28.6 +/- 4.9) microm2/microm] were significantly higher than those of A group [(67 +/- 10) microm2/microm, (16.8 +/- 2.4) microm2/microm, t = 2.938, 3.227, all P < 0.01]. The contents of TNF-alpha and PGE2 in D group [(0.68 +/- 0.25) microg/L, (0.122 +/- 0.030) microg/L] were significantly higher than those in A group [(0.37 +/- 0.09) microg/L, (0.079 +/- 0.018) microg/L, t = 2.683, 3.016, all P < 0.01]. When A group was compared with B group [(0.74 +/- 0.29) microg/L, (0.120 +/- 0.028) microg/L] and C group [(0.71 +/- 0.23) microg/L, (0.117 +/- 0.028) microg/L], there was no significant difference (t = 1.624, 0.472, all P > 0.05) and (t = 0.935, 0.533, all P > 0.05). The contents (light density) of MMP-9 mRNA and TIMP-1 mRNA in D group (0.346 +/- 0.033, 0.361 +/- 0.040) were significantly higher than C group (0.279 +/- 0.015, 0.259 +/- 0.015, t = 2.574, 2.716, all P < 0.01), and so did D group and B group (0.183 +/- 0.025, 0.136 +/- 0.014, t = 2.913, 3.017, all P < 0.01), D group and A group (0.104 +/- 0.007, 0.109 +/- 0.008, t = 3.632, 3.487, all P < 0.01). There were significant correlations between the contents of MMP-9 mRNA and WAt (r = 0.693, P < 0.01), between MMP-9 mRNA and WAm (r = 0.738, P < 0.01), between TIMP-1 mRNA and WAt (r = 0.823, P < 0.01), and between TIMP-1 mRNA and WAm (r = 0.876, P < 0.01). CONCLUSION: AM and AM-derived cytokines are associated with airway remodeling in asthmatic rats. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/17327053/[The_role_of_alveolar_macrophages_in_airway_remodeling_in_asthmatic_rats]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1001-0939&amp;year=2006&amp;vol=29&amp;issue=11&amp;fpage=740 DB - PRIME DP - Unbound Medicine ER -