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Gastroprotective effect of Terminalia arjuna bark on diclofenac sodium induced gastric ulcer.
Chem Biol Interact. 2007 Apr 05; 167(1):71-83.CB

Abstract

AIM

The present study was aimed to evaluate the effect of methanolic extract of Terminalia arjuna (TA) on diclofenac sodium induced gastric ulcer in experimental rats.

METHODS

Animals were induced for gastric ulcer with diclofenac sodium (DIC) (80mg/kg bodyweight in water, orally) and treated orally with TA in various doses ranging from 100mg/kg bodyweight to 500mg/kg bodyweight. The effective dose was 400mg/kg bodyweight, since this dose elicited a maximum reduction in lesion index. The gastroprotective effect of TA was assessed from volume of gastric juice, pH, free and total acidity, pepsin concentration, acid output in gastric juice, the levels of non-protein sulfhydryls (NP-SH), lipid peroxide (LPO), reduced glutathione (GSH), and activities of enzymic antioxidants--super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and myeloperoxidase (MPO) in gastric mucosa. The levels of DNA, protein bound carbohydrate complexes--hexose, hexoseamine, sialic acid, fucose in gastric mucosa and gastric juice and the levels of RNA in gastric mucosa were assessed. The stomach tissues were used for adherent mucus content and also for the histological examination.

RESULTS

A significant reduction in lesion index was observed in ulcer induced animals treated with TA (DIC+TA) compared to ulcerated rats (DIC). A significant increase was observed in pH, NP-SH, GSH, enzymic antioxidants, protein bound carbohydrate complexes, adherent mucus content, nucleic acids with a significant decrease in volume of gastric juice, free and total acidity, pepsin concentration, acid output, LPO levels and MPO activities in DIC+TA rats compared to DIC rats. Histological studies confirmed the gastroprotective activity of TA.

CONCLUSION

From the data presented in this study it could be concluded that T. arjuna acts as an gastroprotective agent probably due to its free radical scavenging activity and cytoprotective nature.

Authors+Show Affiliations

Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17327128

Citation

Devi, Rethinam Sundaresan, et al. "Gastroprotective Effect of Terminalia Arjuna Bark On Diclofenac Sodium Induced Gastric Ulcer." Chemico-biological Interactions, vol. 167, no. 1, 2007, pp. 71-83.
Devi RS, Narayan S, Vani G, et al. Gastroprotective effect of Terminalia arjuna bark on diclofenac sodium induced gastric ulcer. Chem Biol Interact. 2007;167(1):71-83.
Devi, R. S., Narayan, S., Vani, G., & Shyamala Devi, C. S. (2007). Gastroprotective effect of Terminalia arjuna bark on diclofenac sodium induced gastric ulcer. Chemico-biological Interactions, 167(1), 71-83.
Devi RS, et al. Gastroprotective Effect of Terminalia Arjuna Bark On Diclofenac Sodium Induced Gastric Ulcer. Chem Biol Interact. 2007 Apr 5;167(1):71-83. PubMed PMID: 17327128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastroprotective effect of Terminalia arjuna bark on diclofenac sodium induced gastric ulcer. AU - Devi,Rethinam Sundaresan, AU - Narayan,Shoba, AU - Vani,Ganapathy, AU - Shyamala Devi,Chennam Srinivasulu, Y1 - 2007/02/02/ PY - 2006/11/23/received PY - 2007/01/21/revised PY - 2007/01/22/accepted PY - 2007/3/1/pubmed PY - 2007/5/30/medline PY - 2007/3/1/entrez SP - 71 EP - 83 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 167 IS - 1 N2 - AIM: The present study was aimed to evaluate the effect of methanolic extract of Terminalia arjuna (TA) on diclofenac sodium induced gastric ulcer in experimental rats. METHODS: Animals were induced for gastric ulcer with diclofenac sodium (DIC) (80mg/kg bodyweight in water, orally) and treated orally with TA in various doses ranging from 100mg/kg bodyweight to 500mg/kg bodyweight. The effective dose was 400mg/kg bodyweight, since this dose elicited a maximum reduction in lesion index. The gastroprotective effect of TA was assessed from volume of gastric juice, pH, free and total acidity, pepsin concentration, acid output in gastric juice, the levels of non-protein sulfhydryls (NP-SH), lipid peroxide (LPO), reduced glutathione (GSH), and activities of enzymic antioxidants--super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and myeloperoxidase (MPO) in gastric mucosa. The levels of DNA, protein bound carbohydrate complexes--hexose, hexoseamine, sialic acid, fucose in gastric mucosa and gastric juice and the levels of RNA in gastric mucosa were assessed. The stomach tissues were used for adherent mucus content and also for the histological examination. RESULTS: A significant reduction in lesion index was observed in ulcer induced animals treated with TA (DIC+TA) compared to ulcerated rats (DIC). A significant increase was observed in pH, NP-SH, GSH, enzymic antioxidants, protein bound carbohydrate complexes, adherent mucus content, nucleic acids with a significant decrease in volume of gastric juice, free and total acidity, pepsin concentration, acid output, LPO levels and MPO activities in DIC+TA rats compared to DIC rats. Histological studies confirmed the gastroprotective activity of TA. CONCLUSION: From the data presented in this study it could be concluded that T. arjuna acts as an gastroprotective agent probably due to its free radical scavenging activity and cytoprotective nature. SN - 0009-2797 UR - https://www.unboundmedicine.com/medline/citation/17327128/Gastroprotective_effect_of_Terminalia_arjuna_bark_on_diclofenac_sodium_induced_gastric_ulcer_ DB - PRIME DP - Unbound Medicine ER -