Tags

Type your tag names separated by a space and hit enter

Effective two-dimensional counter-current chromatographic method for simultaneous isolation and purification of oridonin and ponicidin from the crude extract of Rabdosia rubescens.
J Chromatogr A. 2007 Mar 30; 1146(1):125-30.JC

Abstract

A preparative two-dimensional counter-current chromatographic system (2D-CCC) for simultaneous separation and purification of oridonin and ponicidin from the crude extract of Rabdosia rubescens was presented. It was based on the use of a high-speed CCC (HSCCC, total column volume V(c)=146ml) instrument in the first dimension (1st-D) and a preparative upright CCC (UCCC, V(c)=1500ml) column in the second dimension (2nd-D). The interface was a homemade column-switching system with a 50-ml sample loop. In this way, almost the whole interested region from the first dimension was transferred on-line to the second dimension for further separation. The use of a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (1:5:1:5 and 3:5:3:5, v/v) in the two dimensions permitted the simultaneous separation of oridonin and ponicidin, making this 2D-CCC system with satisfactory resolution and peak capacity. During about 9h separation, two injections with about a 250mg amount of the crude extract for each, was accomplished using this 2D-CCC system, yielding 60mg of oridonin (1) and 10mg of ponicidin (2) all at a purity of over 95% as determined by HPLC analysis. Their chemical structures were identified by electrospray ionization (ESI) MS, (1)H NMR and (13)C NMR.

Authors+Show Affiliations

Department of Chemistry, Zhejiang University, Hangzhou 310027, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17328904

Citation

Lu, Yanbin, et al. "Effective Two-dimensional Counter-current Chromatographic Method for Simultaneous Isolation and Purification of Oridonin and Ponicidin From the Crude Extract of Rabdosia Rubescens." Journal of Chromatography. A, vol. 1146, no. 1, 2007, pp. 125-30.
Lu Y, Sun C, Liu R, et al. Effective two-dimensional counter-current chromatographic method for simultaneous isolation and purification of oridonin and ponicidin from the crude extract of Rabdosia rubescens. J Chromatogr A. 2007;1146(1):125-30.
Lu, Y., Sun, C., Liu, R., & Pan, Y. (2007). Effective two-dimensional counter-current chromatographic method for simultaneous isolation and purification of oridonin and ponicidin from the crude extract of Rabdosia rubescens. Journal of Chromatography. A, 1146(1), 125-30.
Lu Y, et al. Effective Two-dimensional Counter-current Chromatographic Method for Simultaneous Isolation and Purification of Oridonin and Ponicidin From the Crude Extract of Rabdosia Rubescens. J Chromatogr A. 2007 Mar 30;1146(1):125-30. PubMed PMID: 17328904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effective two-dimensional counter-current chromatographic method for simultaneous isolation and purification of oridonin and ponicidin from the crude extract of Rabdosia rubescens. AU - Lu,Yanbin, AU - Sun,Cuirong, AU - Liu,Rui, AU - Pan,Yuanjiang, Y1 - 2007/02/16/ PY - 2007/01/08/received PY - 2007/01/27/revised PY - 2007/01/30/accepted PY - 2007/3/3/pubmed PY - 2007/5/1/medline PY - 2007/3/3/entrez SP - 125 EP - 30 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1146 IS - 1 N2 - A preparative two-dimensional counter-current chromatographic system (2D-CCC) for simultaneous separation and purification of oridonin and ponicidin from the crude extract of Rabdosia rubescens was presented. It was based on the use of a high-speed CCC (HSCCC, total column volume V(c)=146ml) instrument in the first dimension (1st-D) and a preparative upright CCC (UCCC, V(c)=1500ml) column in the second dimension (2nd-D). The interface was a homemade column-switching system with a 50-ml sample loop. In this way, almost the whole interested region from the first dimension was transferred on-line to the second dimension for further separation. The use of a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (1:5:1:5 and 3:5:3:5, v/v) in the two dimensions permitted the simultaneous separation of oridonin and ponicidin, making this 2D-CCC system with satisfactory resolution and peak capacity. During about 9h separation, two injections with about a 250mg amount of the crude extract for each, was accomplished using this 2D-CCC system, yielding 60mg of oridonin (1) and 10mg of ponicidin (2) all at a purity of over 95% as determined by HPLC analysis. Their chemical structures were identified by electrospray ionization (ESI) MS, (1)H NMR and (13)C NMR. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17328904/Effective_two_dimensional_counter_current_chromatographic_method_for_simultaneous_isolation_and_purification_of_oridonin_and_ponicidin_from_the_crude_extract_of_Rabdosia_rubescens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00222-1 DB - PRIME DP - Unbound Medicine ER -