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Simultaneous determination of vitexin-4''-O-glucoside and vitexin-2''-O-rhamnoside from Hawthorn leaves flavonoids in rat plasma by HPLC method and its application to pharmacokinetic studies.
J Pharm Biomed Anal. 2007 May 09; 44(1):243-9.JP

Abstract

The present study was to investigate the pharmacokinetics of the two similar flavonoid glycosides, vitexin-4''-O-glucoside (VGL) and vitexin-2''-O-rhamnoside (VRH) in rats after intravenous administration of hawthorn leaves flavonoids (HLF). Blood samples were collected via tail vein at time intervals after drug administration and the plasma concentrations of the studied ingredients were analyzed by HPLC after the plasma protein was precipitated directly with methanol. VGL and VRH were successfully separated using a C(18) column with a UV detection at 330 nm and a mobile phase of methanol-acetonitrile-tetrahydrofuran-0.5% acetic acid (1:1:19.4:78.6, v/v/v/v). The assay linearities of VGL and VRH were confirmed over the range 0.23-138.42 and 0.36-218.49 microg/ml, respectively. The accuracy and precision of the two analytes at high, medium and low concentration were within the range of -3.13% to 3.51% and below 4%, the mean assay recoveries of them (n=5) ranged from 96.87% to 101.75% and 96.88% to 103.51% for intra- and inter-day assays and the mean extraction recoveries of them (n=5) varied from 92.68% to 95.74% for VGL and 93.45% to 99.26% for VRH, respectively. After intravenous administration of HLF to rats over the doses range of 10-40 mg/kg, the plasma concentration--time curves of VGL and VRH were both conformed to the three-compartment open pharmacokinetic model and linear pharmacokinetic characteristics.

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Authors+Show Affiliations

Ma G
Department of Clinical Pharmacy, West China School of Pharmacy, Sichuan University, Key Laboratory of Drug Targeting of Ministry of Education, Chengdu 610041, PR China.
Jiang XH
No affiliation info available
Chen Z
No affiliation info available
Ren J
No affiliation info available
Li CR
No affiliation info available
Liu TM
No affiliation info available

MeSH

AnimalsApigeninChromatography, High Pressure LiquidCrataegusDose-Response Relationship, DrugDrug StabilityFemaleFlavonoidsFreezingHalf-LifeInjections, IntravenousLeast-Squares AnalysisMaleMetabolic Clearance RateMethanolMolecular StructurePlant ExtractsPlant LeavesRandom AllocationRatsRats, Sprague-DawleyReproducibility of ResultsTime Factors

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17329058

Citation

Ma, Guo, et al. "Simultaneous Determination of vitexin-4''-O-glucoside and vitexin-2''-O-rhamnoside From Hawthorn Leaves Flavonoids in Rat Plasma By HPLC Method and Its Application to Pharmacokinetic Studies." Journal of Pharmaceutical and Biomedical Analysis, vol. 44, no. 1, 2007, pp. 243-9.
Ma G, Jiang XH, Chen Z, et al. Simultaneous determination of vitexin-4''-O-glucoside and vitexin-2''-O-rhamnoside from Hawthorn leaves flavonoids in rat plasma by HPLC method and its application to pharmacokinetic studies. J Pharm Biomed Anal. 2007;44(1):243-9.
Ma, G., Jiang, X. H., Chen, Z., Ren, J., Li, C. R., & Liu, T. M. (2007). Simultaneous determination of vitexin-4''-O-glucoside and vitexin-2''-O-rhamnoside from Hawthorn leaves flavonoids in rat plasma by HPLC method and its application to pharmacokinetic studies. Journal of Pharmaceutical and Biomedical Analysis, 44(1), 243-9.
Ma G, et al. Simultaneous Determination of vitexin-4''-O-glucoside and vitexin-2''-O-rhamnoside From Hawthorn Leaves Flavonoids in Rat Plasma By HPLC Method and Its Application to Pharmacokinetic Studies. J Pharm Biomed Anal. 2007 May 9;44(1):243-9. PubMed PMID: 17329058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of vitexin-4''-O-glucoside and vitexin-2''-O-rhamnoside from Hawthorn leaves flavonoids in rat plasma by HPLC method and its application to pharmacokinetic studies. AU - Ma,Guo, AU - Jiang,Xue-Hua, AU - Chen,Zhuo, AU - Ren,Jing, AU - Li,Chen-Rui, AU - Liu,Tai-Ming, Y1 - 2007/01/30/ PY - 2006/09/01/received PY - 2007/01/12/revised PY - 2007/01/17/accepted PY - 2007/3/3/pubmed PY - 2007/6/23/medline PY - 2007/3/3/entrez SP - 243 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 44 IS - 1 N2 - The present study was to investigate the pharmacokinetics of the two similar flavonoid glycosides, vitexin-4''-O-glucoside (VGL) and vitexin-2''-O-rhamnoside (VRH) in rats after intravenous administration of hawthorn leaves flavonoids (HLF). Blood samples were collected via tail vein at time intervals after drug administration and the plasma concentrations of the studied ingredients were analyzed by HPLC after the plasma protein was precipitated directly with methanol. VGL and VRH were successfully separated using a C(18) column with a UV detection at 330 nm and a mobile phase of methanol-acetonitrile-tetrahydrofuran-0.5% acetic acid (1:1:19.4:78.6, v/v/v/v). The assay linearities of VGL and VRH were confirmed over the range 0.23-138.42 and 0.36-218.49 microg/ml, respectively. The accuracy and precision of the two analytes at high, medium and low concentration were within the range of -3.13% to 3.51% and below 4%, the mean assay recoveries of them (n=5) ranged from 96.87% to 101.75% and 96.88% to 103.51% for intra- and inter-day assays and the mean extraction recoveries of them (n=5) varied from 92.68% to 95.74% for VGL and 93.45% to 99.26% for VRH, respectively. After intravenous administration of HLF to rats over the doses range of 10-40 mg/kg, the plasma concentration--time curves of VGL and VRH were both conformed to the three-compartment open pharmacokinetic model and linear pharmacokinetic characteristics. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/17329058/Simultaneous_determination_of_vitexin_4''_O_glucoside_and_vitexin_2''_O_rhamnoside_from_Hawthorn_leaves_flavonoids_in_rat_plasma_by_HPLC_method_and_its_application_to_pharmacokinetic_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(07)00067-2 DB - PRIME DP - Unbound Medicine ER -
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