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Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 01; 852(1-2):479-84.JC

Abstract

A sensitive, specific and rapid liquid chromatographic/tandem mass spectrometric (LC/MS/MS) method was developed and validated for quantification of nalmefene in human plasma. An aliquot of 200 microL plasma sample was simply precipitated by 400 microL methanol. Separation of nalmefene and the internal standard hydromorphone from the interferences was achieved on a C(18) column followed by MS/MS detection. The analytes were monitored in the positive ionization mode with a TurboIonspray source. The method had a total chromatographic run time of 4.5 min and linear calibration curves over the concentration range of 10-5000 pg/mL. The lower limit of quantification (LLOQ) was 10 pg/mL. The intra- and inter-day precision was less than 10.1% determined from QC samples at concentrations of 30, 300 and 4500 pg/mL, and the accuracy was within +/-3.4%. As the method was more sensitive (10 times higher) than those reported previously, we investigated the pharmacokinetics of nalmefene in healthy volunteers after a single intravenous injection of low dose (30 microg) of nalmefene hydrochloride for the first time.

Authors+Show Affiliations

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 646 Songtao Road, Shanghai 201203, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17329173

Citation

Li, Ping, et al. "Application of a Sensitive Liquid Chromatographic/tandem Mass Spectrometric Method to Pharmacokinetic Study of Nalmefene in Humans." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 852, no. 1-2, 2007, pp. 479-84.
Li P, Chen X, Dai X, et al. Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;852(1-2):479-84.
Li, P., Chen, X., Dai, X., Wen, A., Zhang, Y., & Zhong, D. (2007). Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 852(1-2), 479-84.
Li P, et al. Application of a Sensitive Liquid Chromatographic/tandem Mass Spectrometric Method to Pharmacokinetic Study of Nalmefene in Humans. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):479-84. PubMed PMID: 17329173.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of a sensitive liquid chromatographic/tandem mass spectrometric method to pharmacokinetic study of nalmefene in humans. AU - Li,Ping, AU - Chen,Xiaoyan, AU - Dai,Xiaojian, AU - Wen,Aidong, AU - Zhang,Yifan, AU - Zhong,Dafang, Y1 - 2007/02/15/ PY - 2006/12/07/received PY - 2007/02/01/revised PY - 2007/02/04/accepted PY - 2007/3/3/pubmed PY - 2007/8/2/medline PY - 2007/3/3/entrez SP - 479 EP - 84 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 852 IS - 1-2 N2 - A sensitive, specific and rapid liquid chromatographic/tandem mass spectrometric (LC/MS/MS) method was developed and validated for quantification of nalmefene in human plasma. An aliquot of 200 microL plasma sample was simply precipitated by 400 microL methanol. Separation of nalmefene and the internal standard hydromorphone from the interferences was achieved on a C(18) column followed by MS/MS detection. The analytes were monitored in the positive ionization mode with a TurboIonspray source. The method had a total chromatographic run time of 4.5 min and linear calibration curves over the concentration range of 10-5000 pg/mL. The lower limit of quantification (LLOQ) was 10 pg/mL. The intra- and inter-day precision was less than 10.1% determined from QC samples at concentrations of 30, 300 and 4500 pg/mL, and the accuracy was within +/-3.4%. As the method was more sensitive (10 times higher) than those reported previously, we investigated the pharmacokinetics of nalmefene in healthy volunteers after a single intravenous injection of low dose (30 microg) of nalmefene hydrochloride for the first time. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17329173/Application_of_a_sensitive_liquid_chromatographic/tandem_mass_spectrometric_method_to_pharmacokinetic_study_of_nalmefene_in_humans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00112-2 DB - PRIME DP - Unbound Medicine ER -