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CE with sequential light-emitting diode-induced fluorescence and electro-chemiluminescence detections for the determination of amino acids and alkaloids.
Electrophoresis. 2007 Apr; 28(7):1092-9.E

Abstract

This paper describes the determination of alkaloids and amino acids (AAs) using CE in conjunction with sequential light-emitting diode-induced fluorescence (LEDIF) and electrochemiluminescence (ECL) detections. In the CE-LEDIF-ECL system, the ECL detector was located in the outlet of the capillary, while the LEDIF detector was positioned 12 cm from the outlet. Naphthalene-2,3-dicarboxaldehyde (NDA) was used to form fluorescent AA-NDA derivatives from AAs possessing primary amino groups, while Ru(bpy)(3) (2+) was used to obtain ECL signals for analytes having secondary and tertiary amino groups. In the presence of poly(ethylene oxide), we accomplished the CE-LEDIF-ECL separation of a mixture of 12 AA-NDA derivatives, anabasine, nicotine, and proline within 11 min. This low-cost CE-LEDIF-ECL system allows the analysis of these AA-NDA derivatives and alkaloids at concentrations in the ranges of 49 nM-0.2 microM and 0.66-4.7 microM, respectively. We applied our CE-LEDIF-ECL system to the analysis of a urine sample and also to tobacco extracts. We obtained good qualitative and quantitative results when using this method with these analytes: the RSDs were below 3.0 and 2.8%, respectively. This CE-LEDIF-ECL system provides the advantages of high efficiency, speed, and sensitivity for the analysis of analytes possessing amino groups.

Authors+Show Affiliations

Chang PL
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Lee KH
No affiliation info available
Hu CC
No affiliation info available
Chang HT
No affiliation info available

MeSH

AdultAlkaloidsAmino AcidsElectrophoresis, CapillaryFluorescenceFluorescent DyesHumansLuminescent MeasurementsMaleNaphthalenesOrganometallic CompoundsPlant ExtractsPolyethylene GlycolsTobacco

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17330227

Citation

Chang, Po-Ling, et al. "CE With Sequential Light-emitting Diode-induced Fluorescence and Electro-chemiluminescence Detections for the Determination of Amino Acids and Alkaloids." Electrophoresis, vol. 28, no. 7, 2007, pp. 1092-9.
Chang PL, Lee KH, Hu CC, et al. CE with sequential light-emitting diode-induced fluorescence and electro-chemiluminescence detections for the determination of amino acids and alkaloids. Electrophoresis. 2007;28(7):1092-9.
Chang, P. L., Lee, K. H., Hu, C. C., & Chang, H. T. (2007). CE with sequential light-emitting diode-induced fluorescence and electro-chemiluminescence detections for the determination of amino acids and alkaloids. Electrophoresis, 28(7), 1092-9.
Chang PL, et al. CE With Sequential Light-emitting Diode-induced Fluorescence and Electro-chemiluminescence Detections for the Determination of Amino Acids and Alkaloids. Electrophoresis. 2007;28(7):1092-9. PubMed PMID: 17330227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CE with sequential light-emitting diode-induced fluorescence and electro-chemiluminescence detections for the determination of amino acids and alkaloids. AU - Chang,Po-Ling, AU - Lee,Kun-Hong, AU - Hu,Cho-Chun, AU - Chang,Huan-Tsung, PY - 2007/3/3/pubmed PY - 2007/7/10/medline PY - 2007/3/3/entrez SP - 1092 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 28 IS - 7 N2 - This paper describes the determination of alkaloids and amino acids (AAs) using CE in conjunction with sequential light-emitting diode-induced fluorescence (LEDIF) and electrochemiluminescence (ECL) detections. In the CE-LEDIF-ECL system, the ECL detector was located in the outlet of the capillary, while the LEDIF detector was positioned 12 cm from the outlet. Naphthalene-2,3-dicarboxaldehyde (NDA) was used to form fluorescent AA-NDA derivatives from AAs possessing primary amino groups, while Ru(bpy)(3) (2+) was used to obtain ECL signals for analytes having secondary and tertiary amino groups. In the presence of poly(ethylene oxide), we accomplished the CE-LEDIF-ECL separation of a mixture of 12 AA-NDA derivatives, anabasine, nicotine, and proline within 11 min. This low-cost CE-LEDIF-ECL system allows the analysis of these AA-NDA derivatives and alkaloids at concentrations in the ranges of 49 nM-0.2 microM and 0.66-4.7 microM, respectively. We applied our CE-LEDIF-ECL system to the analysis of a urine sample and also to tobacco extracts. We obtained good qualitative and quantitative results when using this method with these analytes: the RSDs were below 3.0 and 2.8%, respectively. This CE-LEDIF-ECL system provides the advantages of high efficiency, speed, and sensitivity for the analysis of analytes possessing amino groups. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/17330227/CE_with_sequential_light_emitting_diode_induced_fluorescence_and_electro_chemiluminescence_detections_for_the_determination_of_amino_acids_and_alkaloids_ L2 - https://doi.org/10.1002/elps.200600546 DB - PRIME DP - Unbound Medicine ER -