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Differential immunostimulating effect of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) and interferon gamma (IFNgamma) after severe trauma.
Inflamm Res. 2007 Jan; 56(1):38-44.IR

Abstract

OBJECTIVE

Severe trauma leads to an increased vulnerability to bacterial sepsis. In the present study, we compared the immunostimulating potential of granulocyte-colony stimulating-factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-gamma (IFN-gamma).

DESIGN

Prospective clinical experimental study.

SETTING

University hospital intensive care unit and research facility.

PATIENTS

6 patients with an Injury Severity Score (ISS) of more than 25 points.

INTERVENTIONS

Heparinized blood samples of severely injured patients and 12 healthy volunteers were incubated in vitro with 10 ng/ml GM-CSF, 10 ng/ml G-CSF or 10 ng/ml IFN-gamma for 6 h.

MEASUREMENTS

Flow cytometry: HLA-DR expression on monocytes, B- and T-lymphocytes. ELISA: LPS-induced TNFalpha and IL-10 production.

RESULTS

In all patients reduced cytokine production and HLA-DR expression on monocytes was established. After administration of GM-CSF and IFN-gamma it in vitro, the level of HLA-DR expression on monocytes and the it ex vivo TNFalpha-synthesis increased while only GM-CSF increased significantly IL-10-liberation after LPS-stimulation. However, only IFN-gamma had the capacity to enhance HLA-DR on B- and T-lymphocytes. G-CSF it in vitro had no significant effect on the measured parameter.

CONCLUSIONS

These data suggest that GM-CSF and IFN-gamma may serve to support immune functions in severely injured patients.

Authors+Show Affiliations

Department of Trauma Surgery and section of Trauma-Surgery research, University Hospital of Essen, University Duisburg-Essen, Essen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17334669

Citation

Lendemans, S, et al. "Differential Immunostimulating Effect of Granulocyte-macrophage Colony-stimulating Factor (GM-CSF), Granulocyte Colony-stimulating Factor (G-CSF) and Interferon Gamma (IFNgamma) After Severe Trauma." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 56, no. 1, 2007, pp. 38-44.
Lendemans S, Kreuzfelder E, Waydhas C, et al. Differential immunostimulating effect of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) and interferon gamma (IFNgamma) after severe trauma. Inflamm Res. 2007;56(1):38-44.
Lendemans, S., Kreuzfelder, E., Waydhas, C., Schade, F. U., & Flohé, S. (2007). Differential immunostimulating effect of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) and interferon gamma (IFNgamma) after severe trauma. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 56(1), 38-44.
Lendemans S, et al. Differential Immunostimulating Effect of Granulocyte-macrophage Colony-stimulating Factor (GM-CSF), Granulocyte Colony-stimulating Factor (G-CSF) and Interferon Gamma (IFNgamma) After Severe Trauma. Inflamm Res. 2007;56(1):38-44. PubMed PMID: 17334669.
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TY - JOUR T1 - Differential immunostimulating effect of granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) and interferon gamma (IFNgamma) after severe trauma. AU - Lendemans,S, AU - Kreuzfelder,E, AU - Waydhas,C, AU - Schade,F U, AU - Flohé,S, PY - 2007/3/6/pubmed PY - 2007/5/3/medline PY - 2007/3/6/entrez SP - 38 EP - 44 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm Res VL - 56 IS - 1 N2 - OBJECTIVE: Severe trauma leads to an increased vulnerability to bacterial sepsis. In the present study, we compared the immunostimulating potential of granulocyte-colony stimulating-factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-gamma (IFN-gamma). DESIGN: Prospective clinical experimental study. SETTING: University hospital intensive care unit and research facility. PATIENTS: 6 patients with an Injury Severity Score (ISS) of more than 25 points. INTERVENTIONS: Heparinized blood samples of severely injured patients and 12 healthy volunteers were incubated in vitro with 10 ng/ml GM-CSF, 10 ng/ml G-CSF or 10 ng/ml IFN-gamma for 6 h. MEASUREMENTS: Flow cytometry: HLA-DR expression on monocytes, B- and T-lymphocytes. ELISA: LPS-induced TNFalpha and IL-10 production. RESULTS: In all patients reduced cytokine production and HLA-DR expression on monocytes was established. After administration of GM-CSF and IFN-gamma it in vitro, the level of HLA-DR expression on monocytes and the it ex vivo TNFalpha-synthesis increased while only GM-CSF increased significantly IL-10-liberation after LPS-stimulation. However, only IFN-gamma had the capacity to enhance HLA-DR on B- and T-lymphocytes. G-CSF it in vitro had no significant effect on the measured parameter. CONCLUSIONS: These data suggest that GM-CSF and IFN-gamma may serve to support immune functions in severely injured patients. SN - 1023-3830 UR - https://www.unboundmedicine.com/medline/citation/17334669/Differential_immunostimulating_effect_of_granulocyte_macrophage_colony_stimulating_factor__GM_CSF__granulocyte_colony_stimulating_factor__G_CSF__and_interferon_gamma__IFNgamma__after_severe_trauma_ L2 - https://dx.doi.org/10.1007/s00011-007-6069-7 DB - PRIME DP - Unbound Medicine ER -