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Continuous fermentation of wheat-supplemented lignocellulose hydrolysate with different types of cell retention.
Biotechnol Bioeng. 2007 Sep 01; 98(1):80-90.BB

Abstract

Medium supplementation and process alternatives for fuel ethanol production from dilute acid lignocellulose hydrolysate were investigated. Dilute acid lignocellulose hydrolysate supplemented with enzymatically hydrolysed wheat flour could sustain continuous anaerobic cultivation of Saccharomyces cerevisiae ATCC 96581 if further supplemented with ammonium sulphate and biotin. This medium composition allowed for a hexose utilisation of 73% and an ethanol production of 36 mmol l(-1) h(-1) in chemostat cultivation at dilution rate 0.10 h(-1). Three different methods for cell retention were compared for improved fermentation of supplemented lignocellulose hydrolysate: cell recirculation by filtration, cell recirculation by sedimentation and cell immobilisation in calcium alginate. All three cell retention methods improved the hexose conversion and increased the volumetric ethanol production rate. Recirculation of 75% of the bioreactor outlet flow by filtration improved the hexose utilisation from 76% to 94%. Sedimentation turned out to be an efficient method for cell separation; the cell concentration in the reactor was 32 times higher than in the outflow after 60 h of substrate feeding. However, chemostat and continuous cell recirculation cultures became severely inhibited when the dilution rate was increased to 0.20 h(-1). In contrast, an immobilised system kept producing ethanol at a stable level also at dilution rate 0.30 h(-1).

Authors+Show Affiliations

Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17335066

Citation

Brandberg, Tomas, et al. "Continuous Fermentation of Wheat-supplemented Lignocellulose Hydrolysate With Different Types of Cell Retention." Biotechnology and Bioengineering, vol. 98, no. 1, 2007, pp. 80-90.
Brandberg T, Karimi K, Taherzadeh MJ, et al. Continuous fermentation of wheat-supplemented lignocellulose hydrolysate with different types of cell retention. Biotechnol Bioeng. 2007;98(1):80-90.
Brandberg, T., Karimi, K., Taherzadeh, M. J., Franzén, C. J., & Gustafsson, L. (2007). Continuous fermentation of wheat-supplemented lignocellulose hydrolysate with different types of cell retention. Biotechnology and Bioengineering, 98(1), 80-90.
Brandberg T, et al. Continuous Fermentation of Wheat-supplemented Lignocellulose Hydrolysate With Different Types of Cell Retention. Biotechnol Bioeng. 2007 Sep 1;98(1):80-90. PubMed PMID: 17335066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Continuous fermentation of wheat-supplemented lignocellulose hydrolysate with different types of cell retention. AU - Brandberg,Tomas, AU - Karimi,Keikhosro, AU - Taherzadeh,Mohammad J, AU - Franzén,Carl Johan, AU - Gustafsson,Lena, PY - 2007/3/6/pubmed PY - 2007/10/20/medline PY - 2007/3/6/entrez SP - 80 EP - 90 JF - Biotechnology and bioengineering JO - Biotechnol Bioeng VL - 98 IS - 1 N2 - Medium supplementation and process alternatives for fuel ethanol production from dilute acid lignocellulose hydrolysate were investigated. Dilute acid lignocellulose hydrolysate supplemented with enzymatically hydrolysed wheat flour could sustain continuous anaerobic cultivation of Saccharomyces cerevisiae ATCC 96581 if further supplemented with ammonium sulphate and biotin. This medium composition allowed for a hexose utilisation of 73% and an ethanol production of 36 mmol l(-1) h(-1) in chemostat cultivation at dilution rate 0.10 h(-1). Three different methods for cell retention were compared for improved fermentation of supplemented lignocellulose hydrolysate: cell recirculation by filtration, cell recirculation by sedimentation and cell immobilisation in calcium alginate. All three cell retention methods improved the hexose conversion and increased the volumetric ethanol production rate. Recirculation of 75% of the bioreactor outlet flow by filtration improved the hexose utilisation from 76% to 94%. Sedimentation turned out to be an efficient method for cell separation; the cell concentration in the reactor was 32 times higher than in the outflow after 60 h of substrate feeding. However, chemostat and continuous cell recirculation cultures became severely inhibited when the dilution rate was increased to 0.20 h(-1). In contrast, an immobilised system kept producing ethanol at a stable level also at dilution rate 0.30 h(-1). SN - 0006-3592 UR - https://www.unboundmedicine.com/medline/citation/17335066/Continuous_fermentation_of_wheat_supplemented_lignocellulose_hydrolysate_with_different_types_of_cell_retention_ DB - PRIME DP - Unbound Medicine ER -