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Are Phragmites australis enzymes involved in the degradation of the textile azo dye acid orange 7?
Bioresour Technol. 2008 Jan; 99(2):243-51.BT

Abstract

The role of antioxidant and detoxification enzymes of Phragmites australis, in the degradation of an azo dye, acid orange 7 (AO7), was studied. Activities of several enzymes involved in plant protection against stress were assayed through the activity characterization of superoxide dismutase (SOD), peroxidases (POD), catalase (CAT), ascorbate peroxidase (APOX), dehydroascorbate reductase (DHAR) and glutathione S-transferase (GST), obtained from P. australis crude extracts of leaves, stems and roots. A sub-surface vertical flow constructed wetland, planted with P. australis was used to test the plants response to the AO7 exposure at two different concentrations (130 and 700 mg l(-1)). An activity increase was detected for an AO7 concentration of 130 mg l(-1) for most enzymes studied (SOD, CAT and APOX), especially in leaves, suggesting a response of the reactive oxygen species scavenging enzymes to the chemical stress imposed. GST activity increase in this situation can also be interpreted as an activation of the detoxification pathway and subsequent AO7 conjugation. A totally different behaviour was observed for AO7 at 700 mg l(-1). An evident decrease in activity was observed for SOD, CAT, APOX and GST, probably due to enzymatic inhibition by AO7. Contrarily, DHAR activity augmented drastically in this situation. POD activity was not greatly affected during trial. Altogether these results suggest that P. australis effectively uses the ascorbate-glutathione pathway for the detoxification of AO7.

Authors+Show Affiliations

Institute of Biotechnology and Bioengineering, Centre of Biological and Chemical Engineering, Instituto Superior Técnico, Av Rovisco Pais, 1049-001, Lisbon, Portugal.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17336060

Citation

Carias, Cátia C., et al. "Are Phragmites Australis Enzymes Involved in the Degradation of the Textile Azo Dye Acid Orange 7?" Bioresource Technology, vol. 99, no. 2, 2008, pp. 243-51.
Carias CC, Novais JM, Martins-Dias S. Are Phragmites australis enzymes involved in the degradation of the textile azo dye acid orange 7? Bioresour Technol. 2008;99(2):243-51.
Carias, C. C., Novais, J. M., & Martins-Dias, S. (2008). Are Phragmites australis enzymes involved in the degradation of the textile azo dye acid orange 7? Bioresource Technology, 99(2), 243-51.
Carias CC, Novais JM, Martins-Dias S. Are Phragmites Australis Enzymes Involved in the Degradation of the Textile Azo Dye Acid Orange 7. Bioresour Technol. 2008;99(2):243-51. PubMed PMID: 17336060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Are Phragmites australis enzymes involved in the degradation of the textile azo dye acid orange 7? AU - Carias,Cátia C, AU - Novais,Júlio M, AU - Martins-Dias,Susete, Y1 - 2007/03/01/ PY - 2006/07/24/received PY - 2006/12/29/revised PY - 2006/12/30/accepted PY - 2007/3/6/pubmed PY - 2008/1/8/medline PY - 2007/3/6/entrez SP - 243 EP - 51 JF - Bioresource technology JO - Bioresour Technol VL - 99 IS - 2 N2 - The role of antioxidant and detoxification enzymes of Phragmites australis, in the degradation of an azo dye, acid orange 7 (AO7), was studied. Activities of several enzymes involved in plant protection against stress were assayed through the activity characterization of superoxide dismutase (SOD), peroxidases (POD), catalase (CAT), ascorbate peroxidase (APOX), dehydroascorbate reductase (DHAR) and glutathione S-transferase (GST), obtained from P. australis crude extracts of leaves, stems and roots. A sub-surface vertical flow constructed wetland, planted with P. australis was used to test the plants response to the AO7 exposure at two different concentrations (130 and 700 mg l(-1)). An activity increase was detected for an AO7 concentration of 130 mg l(-1) for most enzymes studied (SOD, CAT and APOX), especially in leaves, suggesting a response of the reactive oxygen species scavenging enzymes to the chemical stress imposed. GST activity increase in this situation can also be interpreted as an activation of the detoxification pathway and subsequent AO7 conjugation. A totally different behaviour was observed for AO7 at 700 mg l(-1). An evident decrease in activity was observed for SOD, CAT, APOX and GST, probably due to enzymatic inhibition by AO7. Contrarily, DHAR activity augmented drastically in this situation. POD activity was not greatly affected during trial. Altogether these results suggest that P. australis effectively uses the ascorbate-glutathione pathway for the detoxification of AO7. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/17336060/Are_Phragmites_australis_enzymes_involved_in_the_degradation_of_the_textile_azo_dye_acid_orange_7 L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(07)00017-X DB - PRIME DP - Unbound Medicine ER -