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The protective effects of 17beta-estradiol on hepatic ischemia-reperfusion injury in rat model, associated with regulation of heat-shock protein expression.
J Surg Res. 2007 Jun 01; 140(1):67-76.JS

Abstract

BACKGROUND

Ischemia-reperfusion (I/R) injury, which was commonly seen in the field of hepatic surgical intervention, impaired liver regeneration and predisposed to liver failure. Previous studies have shown gender dimorphic response of the liver for various hepatic stresses including I/R injury, hemorrhagic shock-resuscitation, liver cirrhosis, endotoxemia, and chronic alcoholic consumption, and demonstrated gender dimorphism in hepatocellular dysfunction after experimental trauma and hemorrhage. The objective of this study was to examine the hypothesis that the protective effects of 17beta-estradiol (E2) in hepatic I/R injury were associated with increasing heat-shock protein 70 expression.

MATERIALS AND METHODS

Sprague-Dawley male and female rats were randomly divided into male and female sham, I/R, and E2 + I/R groups. The model of reduced-size liver ischemia and reperfusion was used. Except for the sham-operated groups, all rats were subjected to 70% liver ischemia for 45 min followed by resection of the remaining 30% nonischemic lobes and reperfusion of ischemic tissue. For each group, five rats were used to investigate the survival during a week after operation; blood samples and liver tissues were obtained in the remaining animals after 3, 12, and 24 h of reperfusion to assess serum alanine aminotransferase, aspartate aminotransferase, liver tissue NO(2)(-) + NO(3)(-), malondialdehyde content, superoxide dismutase, nitric oxide synthase, and myeloperoxidase activity, Hsp70 expression, and apoptosis ratio.

RESULTS

Compared with I/R groups, male and female E2 + I/R groups showed less I/R-induced injury, and SOD and eNOS activity and Hsp70 expression were increased significantly (P < 0.01). A higher rate of apoptosis was observed in the I/R group versus the E2 + I/R group, a significant increase of MDA, NO(2)(-) + NO(3)(-), and MPO of liver tissues and serum transaminase were also observed in the I/R group versus the E2 + I/R group. The survival rate was significantly higher in the male E2 + I/R group than in the male I/R group.

CONCLUSION

E2 pretreatment had protective effects on liver in hepatic I/R injury. The mechanism of this protection might be related to overexpression of Hsp70.

Authors+Show Affiliations

Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17336333

Citation

Shen, Shi-Qiang, et al. "The Protective Effects of 17beta-estradiol On Hepatic Ischemia-reperfusion Injury in Rat Model, Associated With Regulation of Heat-shock Protein Expression." The Journal of Surgical Research, vol. 140, no. 1, 2007, pp. 67-76.
Shen SQ, Zhang Y, Xiong CL. The protective effects of 17beta-estradiol on hepatic ischemia-reperfusion injury in rat model, associated with regulation of heat-shock protein expression. J Surg Res. 2007;140(1):67-76.
Shen, S. Q., Zhang, Y., & Xiong, C. L. (2007). The protective effects of 17beta-estradiol on hepatic ischemia-reperfusion injury in rat model, associated with regulation of heat-shock protein expression. The Journal of Surgical Research, 140(1), 67-76.
Shen SQ, Zhang Y, Xiong CL. The Protective Effects of 17beta-estradiol On Hepatic Ischemia-reperfusion Injury in Rat Model, Associated With Regulation of Heat-shock Protein Expression. J Surg Res. 2007 Jun 1;140(1):67-76. PubMed PMID: 17336333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The protective effects of 17beta-estradiol on hepatic ischemia-reperfusion injury in rat model, associated with regulation of heat-shock protein expression. AU - Shen,Shi-Qiang, AU - Zhang,Yuan, AU - Xiong,Cheng-Long, Y1 - 2007/03/02/ PY - 2006/08/07/received PY - 2006/09/28/revised PY - 2006/10/16/accepted PY - 2007/3/6/pubmed PY - 2007/6/28/medline PY - 2007/3/6/entrez SP - 67 EP - 76 JF - The Journal of surgical research JO - J Surg Res VL - 140 IS - 1 N2 - BACKGROUND: Ischemia-reperfusion (I/R) injury, which was commonly seen in the field of hepatic surgical intervention, impaired liver regeneration and predisposed to liver failure. Previous studies have shown gender dimorphic response of the liver for various hepatic stresses including I/R injury, hemorrhagic shock-resuscitation, liver cirrhosis, endotoxemia, and chronic alcoholic consumption, and demonstrated gender dimorphism in hepatocellular dysfunction after experimental trauma and hemorrhage. The objective of this study was to examine the hypothesis that the protective effects of 17beta-estradiol (E2) in hepatic I/R injury were associated with increasing heat-shock protein 70 expression. MATERIALS AND METHODS: Sprague-Dawley male and female rats were randomly divided into male and female sham, I/R, and E2 + I/R groups. The model of reduced-size liver ischemia and reperfusion was used. Except for the sham-operated groups, all rats were subjected to 70% liver ischemia for 45 min followed by resection of the remaining 30% nonischemic lobes and reperfusion of ischemic tissue. For each group, five rats were used to investigate the survival during a week after operation; blood samples and liver tissues were obtained in the remaining animals after 3, 12, and 24 h of reperfusion to assess serum alanine aminotransferase, aspartate aminotransferase, liver tissue NO(2)(-) + NO(3)(-), malondialdehyde content, superoxide dismutase, nitric oxide synthase, and myeloperoxidase activity, Hsp70 expression, and apoptosis ratio. RESULTS: Compared with I/R groups, male and female E2 + I/R groups showed less I/R-induced injury, and SOD and eNOS activity and Hsp70 expression were increased significantly (P < 0.01). A higher rate of apoptosis was observed in the I/R group versus the E2 + I/R group, a significant increase of MDA, NO(2)(-) + NO(3)(-), and MPO of liver tissues and serum transaminase were also observed in the I/R group versus the E2 + I/R group. The survival rate was significantly higher in the male E2 + I/R group than in the male I/R group. CONCLUSION: E2 pretreatment had protective effects on liver in hepatic I/R injury. The mechanism of this protection might be related to overexpression of Hsp70. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/17336333/The_protective_effects_of_17beta_estradiol_on_hepatic_ischemia_reperfusion_injury_in_rat_model_associated_with_regulation_of_heat_shock_protein_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(06)00557-9 DB - PRIME DP - Unbound Medicine ER -