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Rodent stroke induced by photochemical occlusion of proximal middle cerebral artery: evolution monitored with MR imaging and histopathology.
Eur J Radiol. 2007 Jul; 63(1):68-75.EJ

Abstract

PURPOSE

To longitudinally investigate stroke in rats after photothrombotic occlusion of proximal middle cerebral artery (MCA) with magnetic resonance imaging (MRI) in correlation with histopathology.

MATERIALS AND METHODS

Forty-two rats were subjected to photochemical MCA occlusion and MRI at 1.5T, and sacrificed in seven groups (n=6 each) at the following time points: 1, 3, 6 and 12h, and at day 1, 3 and 9. T2-weighted (T2WI) and diffusion-weighted imaging (DWI) with apparent diffusion coefficient (ADC) map was performed in all rats. Contrast-enhanced T1-weighted imaging (CE-T1WI) was compared to intravital staining with Evans blue in one group for assessing blood-brain barrier (BBB) integrity. The brain was stained histochemically with triphenyl tetrazolium chloride (TTC) and processed for pathological assessment. The evolutional changes of relative lesion volume, signal intensity (SI), and the BBB integrity on MRI with corresponding histopathology were evaluated.

RESULTS

The ischemic lesion volume reached a maximum around 12h to day 1 as visualized successively by DWI, ADC map and T2WI, implicating the evolving pathology from cytotoxic edema through vasogenic edema to tissue death. The ADC of brain infarction underwent a significant reversion after 12h, reflecting the colliquative necrosis. On CE-T1WI, BBB leakage peaked at 6h and at day 3 with a transitional partial recovery around 24h. The infarct volume on T2WI, DWI and ADC map matched well with that on TTC staining at 12h and at day 1 (p>0.05).

CONCLUSION

The evolution of the present photothrombotic stroke model in rats could be characterized by MRI. The obtained information may help longitudinal studies of cerebral ischemia and anti-stroke agents using the same model.

Authors+Show Affiliations

Department of Radiology, Faculty of Medicine, Catholic University of Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17337149

Citation

Chen, Feng, et al. "Rodent Stroke Induced By Photochemical Occlusion of Proximal Middle Cerebral Artery: Evolution Monitored With MR Imaging and Histopathology." European Journal of Radiology, vol. 63, no. 1, 2007, pp. 68-75.
Chen F, Suzuki Y, Nagai N, et al. Rodent stroke induced by photochemical occlusion of proximal middle cerebral artery: evolution monitored with MR imaging and histopathology. Eur J Radiol. 2007;63(1):68-75.
Chen, F., Suzuki, Y., Nagai, N., Jin, L., Yu, J., Wang, H., Marchal, G., & Ni, Y. (2007). Rodent stroke induced by photochemical occlusion of proximal middle cerebral artery: evolution monitored with MR imaging and histopathology. European Journal of Radiology, 63(1), 68-75.
Chen F, et al. Rodent Stroke Induced By Photochemical Occlusion of Proximal Middle Cerebral Artery: Evolution Monitored With MR Imaging and Histopathology. Eur J Radiol. 2007;63(1):68-75. PubMed PMID: 17337149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rodent stroke induced by photochemical occlusion of proximal middle cerebral artery: evolution monitored with MR imaging and histopathology. AU - Chen,Feng, AU - Suzuki,Yasuhiro, AU - Nagai,Nobuo, AU - Jin,Lixin, AU - Yu,Jie, AU - Wang,Huaijun, AU - Marchal,Guy, AU - Ni,Yicheng, Y1 - 2007/03/06/ PY - 2006/10/04/received PY - 2007/01/03/revised PY - 2007/01/04/accepted PY - 2007/3/6/pubmed PY - 2007/12/7/medline PY - 2007/3/6/entrez SP - 68 EP - 75 JF - European journal of radiology JO - Eur J Radiol VL - 63 IS - 1 N2 - PURPOSE: To longitudinally investigate stroke in rats after photothrombotic occlusion of proximal middle cerebral artery (MCA) with magnetic resonance imaging (MRI) in correlation with histopathology. MATERIALS AND METHODS: Forty-two rats were subjected to photochemical MCA occlusion and MRI at 1.5T, and sacrificed in seven groups (n=6 each) at the following time points: 1, 3, 6 and 12h, and at day 1, 3 and 9. T2-weighted (T2WI) and diffusion-weighted imaging (DWI) with apparent diffusion coefficient (ADC) map was performed in all rats. Contrast-enhanced T1-weighted imaging (CE-T1WI) was compared to intravital staining with Evans blue in one group for assessing blood-brain barrier (BBB) integrity. The brain was stained histochemically with triphenyl tetrazolium chloride (TTC) and processed for pathological assessment. The evolutional changes of relative lesion volume, signal intensity (SI), and the BBB integrity on MRI with corresponding histopathology were evaluated. RESULTS: The ischemic lesion volume reached a maximum around 12h to day 1 as visualized successively by DWI, ADC map and T2WI, implicating the evolving pathology from cytotoxic edema through vasogenic edema to tissue death. The ADC of brain infarction underwent a significant reversion after 12h, reflecting the colliquative necrosis. On CE-T1WI, BBB leakage peaked at 6h and at day 3 with a transitional partial recovery around 24h. The infarct volume on T2WI, DWI and ADC map matched well with that on TTC staining at 12h and at day 1 (p>0.05). CONCLUSION: The evolution of the present photothrombotic stroke model in rats could be characterized by MRI. The obtained information may help longitudinal studies of cerebral ischemia and anti-stroke agents using the same model. SN - 0720-048X UR - https://www.unboundmedicine.com/medline/citation/17337149/Rodent_stroke_induced_by_photochemical_occlusion_of_proximal_middle_cerebral_artery:_evolution_monitored_with_MR_imaging_and_histopathology_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0720-048X(07)00025-3 DB - PRIME DP - Unbound Medicine ER -