Tags

Type your tag names separated by a space and hit enter

Separation and determination of four active components in medicinal preparations by flow injection-capillary electrophoresis.
J Pharm Biomed Anal. 2007 Apr 11; 43(5):1700-5.JP

Abstract

A simple, rapid and accurate method for the separation and determination of paracetamol (Par), pseudoephedrine hydrochloride (Pse), dextromethorphan hydrobromide (Dex) and chlorphenamine hydrogen maleate (Chl) was developed by combination of flow injection and capillary zone electrophoresis for the first time. The analysis was carried out using an unmodified fused-silica capillary (75 mm x 75 microm i.d. x 375 microm o.d., effective separation length of 45 mm) and direct ultraviolet detection at 214 nm, 1.0 kV applied voltage. The optimized running buffer composed of 75 mM sodium borate-15% (v/v) acetonitrile (ACN) (pH* 9.30) was applied for the separation of the four analytes. The separation was achieved in 4.5 min. The sample throughput rate could reach up to 19 h(-1). The repeatability (defined as relative standard deviation) was 0.6%, 1.0%, 2.1%, 1.9% with peak height evaluation and 0.7%, 1.8%, 0.7%, 1.1% with peak area evaluation for Par, Pse, Dex and Chl, respectively. The limits of detection (S/N=3) were 0.22 microg/ml, 0.29 microg/ml, 0.42 microg/ml and 0.70 microg/ml for Par, Pse, Dex and Chl, respectively. The method was successfully applied to determine the four compounds in three cold medicines with recoveries in the range of 97.18-105.15%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17337151

Citation

Liu, Xiumei, et al. "Separation and Determination of Four Active Components in Medicinal Preparations By Flow Injection-capillary Electrophoresis." Journal of Pharmaceutical and Biomedical Analysis, vol. 43, no. 5, 2007, pp. 1700-5.
Liu X, Liu L, Chen H, et al. Separation and determination of four active components in medicinal preparations by flow injection-capillary electrophoresis. J Pharm Biomed Anal. 2007;43(5):1700-5.
Liu, X., Liu, L., Chen, H., & Chen, X. (2007). Separation and determination of four active components in medicinal preparations by flow injection-capillary electrophoresis. Journal of Pharmaceutical and Biomedical Analysis, 43(5), 1700-5.
Liu X, et al. Separation and Determination of Four Active Components in Medicinal Preparations By Flow Injection-capillary Electrophoresis. J Pharm Biomed Anal. 2007 Apr 11;43(5):1700-5. PubMed PMID: 17337151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of four active components in medicinal preparations by flow injection-capillary electrophoresis. AU - Liu,Xiumei, AU - Liu,Lihong, AU - Chen,Hongli, AU - Chen,Xingguo, Y1 - 2007/01/08/ PY - 2006/09/08/received PY - 2006/12/24/revised PY - 2007/01/02/accepted PY - 2007/3/6/pubmed PY - 2007/6/19/medline PY - 2007/3/6/entrez SP - 1700 EP - 5 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 43 IS - 5 N2 - A simple, rapid and accurate method for the separation and determination of paracetamol (Par), pseudoephedrine hydrochloride (Pse), dextromethorphan hydrobromide (Dex) and chlorphenamine hydrogen maleate (Chl) was developed by combination of flow injection and capillary zone electrophoresis for the first time. The analysis was carried out using an unmodified fused-silica capillary (75 mm x 75 microm i.d. x 375 microm o.d., effective separation length of 45 mm) and direct ultraviolet detection at 214 nm, 1.0 kV applied voltage. The optimized running buffer composed of 75 mM sodium borate-15% (v/v) acetonitrile (ACN) (pH* 9.30) was applied for the separation of the four analytes. The separation was achieved in 4.5 min. The sample throughput rate could reach up to 19 h(-1). The repeatability (defined as relative standard deviation) was 0.6%, 1.0%, 2.1%, 1.9% with peak height evaluation and 0.7%, 1.8%, 0.7%, 1.1% with peak area evaluation for Par, Pse, Dex and Chl, respectively. The limits of detection (S/N=3) were 0.22 microg/ml, 0.29 microg/ml, 0.42 microg/ml and 0.70 microg/ml for Par, Pse, Dex and Chl, respectively. The method was successfully applied to determine the four compounds in three cold medicines with recoveries in the range of 97.18-105.15%. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/17337151/Separation_and_determination_of_four_active_components_in_medicinal_preparations_by_flow_injection_capillary_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(07)00022-2 DB - PRIME DP - Unbound Medicine ER -