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[Reassortment and gene interactions in the crossing of low-pathogenic avian influenza H5 virus with human influenza virus].
Vopr Virusol. 2007 Jan-Feb; 52(1):23-8.VV

Abstract

The reassortants obtained via the crossing of highly productive influenza virus A/Puerto Rico/8/34 (H1N1) strain and the low pathogenic avian influenza virus A/Duck/Primorie/2621/2001 (H5N2) strain were genotyped and characterized. The H5N2 reassortant having 6 genes from A/Puerto Rico/8/34 virus has the high level of reproduction in chick embryos, while slightly more moderate than in the parent A/Puerto Rico/8/34 strain. The reproduction of the H5N1 reassortant that had 7 genes from A/Puerto Rico/8134 virus was very low. The serial passage selection allowed the investigators to obtain the H5N1 strain that was reproductively close to the H5N2 reassortant. This variant had one amino acid substitution in hemagglutinin (N244D, H3 numbering) and a lower affinity for fetuin. By the level of virulence to mice, the H5N1 and H5N2 reassortants were close to A/Puerto Rico/8/34 virus and greatly differed in this respect from low virulent A/Duck/Primorie/2621/2001 (H5N2). The results are discussed in connection with the problem of vaccination when there is a threat for H5N1 virus subtype-caused pandemic.

Authors

Kochergin-Nikitskiĭ KS
No affiliation info available
Rudneva IA
No affiliation info available
Timofeeva TA
No affiliation info available
Il'iushina NA
No affiliation info available
Varich NL
No affiliation info available
Vlasova AN
No affiliation info available
Kaverin NV
No affiliation info available
L'vov DK
No affiliation info available

MeSH

AnimalsChick EmbryoGenes, ViralHemagglutinins, ViralHumansInfluenza A Virus, H1N1 SubtypeInfluenza A Virus, H5N1 SubtypeInfluenza A Virus, H5N2 SubtypeInfluenza A virusInfluenza, HumanMiceReassortant VirusesSelection, GeneticSerial PassageVirus CultivationVirus Replicationalpha-Fetoproteins

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

rus

PubMed ID

17338230

Citation

Kochergin-Nikitskiĭ, K S., et al. "[Reassortment and Gene Interactions in the Crossing of Low-pathogenic Avian Influenza H5 Virus With Human Influenza Virus]." Voprosy Virusologii, vol. 52, no. 1, 2007, pp. 23-8.
Kochergin-Nikitskiĭ KS, Rudneva IA, Timofeeva TA, et al. [Reassortment and gene interactions in the crossing of low-pathogenic avian influenza H5 virus with human influenza virus]. Vopr Virusol. 2007;52(1):23-8.
Kochergin-Nikitskiĭ, K. S., Rudneva, I. A., Timofeeva, T. A., Il'iushina, N. A., Varich, N. L., Vlasova, A. N., Kaverin, N. V., & L'vov, D. K. (2007). [Reassortment and gene interactions in the crossing of low-pathogenic avian influenza H5 virus with human influenza virus]. Voprosy Virusologii, 52(1), 23-8.
Kochergin-Nikitskiĭ KS, et al. [Reassortment and Gene Interactions in the Crossing of Low-pathogenic Avian Influenza H5 Virus With Human Influenza Virus]. Vopr Virusol. 2007 Jan-Feb;52(1):23-8. PubMed PMID: 17338230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Reassortment and gene interactions in the crossing of low-pathogenic avian influenza H5 virus with human influenza virus]. AU - Kochergin-Nikitskiĭ,K S, AU - Rudneva,I A, AU - Timofeeva,T A, AU - Il'iushina,N A, AU - Varich,N L, AU - Vlasova,A N, AU - Kaverin,N V, AU - L'vov,D K, PY - 2007/3/7/pubmed PY - 2007/4/6/medline PY - 2007/3/7/entrez SP - 23 EP - 8 JF - Voprosy virusologii JO - Vopr Virusol VL - 52 IS - 1 N2 - The reassortants obtained via the crossing of highly productive influenza virus A/Puerto Rico/8/34 (H1N1) strain and the low pathogenic avian influenza virus A/Duck/Primorie/2621/2001 (H5N2) strain were genotyped and characterized. The H5N2 reassortant having 6 genes from A/Puerto Rico/8/34 virus has the high level of reproduction in chick embryos, while slightly more moderate than in the parent A/Puerto Rico/8/34 strain. The reproduction of the H5N1 reassortant that had 7 genes from A/Puerto Rico/8134 virus was very low. The serial passage selection allowed the investigators to obtain the H5N1 strain that was reproductively close to the H5N2 reassortant. This variant had one amino acid substitution in hemagglutinin (N244D, H3 numbering) and a lower affinity for fetuin. By the level of virulence to mice, the H5N1 and H5N2 reassortants were close to A/Puerto Rico/8/34 virus and greatly differed in this respect from low virulent A/Duck/Primorie/2621/2001 (H5N2). The results are discussed in connection with the problem of vaccination when there is a threat for H5N1 virus subtype-caused pandemic. SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/17338230/[Reassortment_and_gene_interactions_in_the_crossing_of_low_pathogenic_avian_influenza_H5_virus_with_human_influenza_virus]_ DB - PRIME DP - Unbound Medicine ER -