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GC-MS quantification of ketamine, norketamine, and dehydronorketamine in urine specimens and comparative study using ELISA as the preliminary test methodology.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 01; 852(1-2):443-9.JC

Abstract

An automated solid-phase extraction procedure combined with the gas chromatography-mass spectrometry (GC-MS) methodology, without derivatization, has been developed for the determination of ketamine (K), norketamine (NK), and dehydronorketamine (DHNK) in urine. The analytical approach is simple and rapid, yet reliable, achieving good linearity (r(2)>0.999 over the concentration range of 30 to 1000 ng/mL), sensitivity (limits of quantification = 15, 10, and 20 ng/mL for K, NK, and DHNK, respectively), accuracy (90-104%), and precision (RSD<8.1%) for all analytes. Two hundred and six urine specimens collected from suspected drug users were analyzed by this protocol and also screened by Neogen ELISA method to evaluate the efficiency as well as the compatibility of these two methods. Neogen ELISA showed high efficiency (98.1%), high sensitivity (90.9%), high specificity (98.9%), low false-positive rate (1.1%), and moderate false-negative rate (9.1%), adopting 10 ng/mL K as the cutoff. Neogen ELISA screening followed by GC-MS analysis appeared to be a good screening-confirmation test scheme for the analysis of K in urine. Twenty of the 22 positive urine specimens contained all three analytes simultaneously, with DHNK showing the highest and K the lowest concentrations.

Authors+Show Affiliations

National Bureau of Controlled Drugs, Department of Health, Taipei, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17339137

Citation

Cheng, Pai-Sheng, et al. "GC-MS Quantification of Ketamine, Norketamine, and Dehydronorketamine in Urine Specimens and Comparative Study Using ELISA as the Preliminary Test Methodology." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 852, no. 1-2, 2007, pp. 443-9.
Cheng PS, Fu CY, Lee CH, et al. GC-MS quantification of ketamine, norketamine, and dehydronorketamine in urine specimens and comparative study using ELISA as the preliminary test methodology. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;852(1-2):443-9.
Cheng, P. S., Fu, C. Y., Lee, C. H., Liu, C., & Chien, C. S. (2007). GC-MS quantification of ketamine, norketamine, and dehydronorketamine in urine specimens and comparative study using ELISA as the preliminary test methodology. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 852(1-2), 443-9.
Cheng PS, et al. GC-MS Quantification of Ketamine, Norketamine, and Dehydronorketamine in Urine Specimens and Comparative Study Using ELISA as the Preliminary Test Methodology. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):443-9. PubMed PMID: 17339137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GC-MS quantification of ketamine, norketamine, and dehydronorketamine in urine specimens and comparative study using ELISA as the preliminary test methodology. AU - Cheng,Pai-Sheng, AU - Fu,Chien-Yu, AU - Lee,Choung-Huei, AU - Liu,Chiareiy, AU - Chien,Chun-Sheng, Y1 - 2007/02/15/ PY - 2006/09/18/received PY - 2007/01/26/revised PY - 2007/02/02/accepted PY - 2007/3/7/pubmed PY - 2007/8/2/medline PY - 2007/3/7/entrez SP - 443 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 852 IS - 1-2 N2 - An automated solid-phase extraction procedure combined with the gas chromatography-mass spectrometry (GC-MS) methodology, without derivatization, has been developed for the determination of ketamine (K), norketamine (NK), and dehydronorketamine (DHNK) in urine. The analytical approach is simple and rapid, yet reliable, achieving good linearity (r(2)>0.999 over the concentration range of 30 to 1000 ng/mL), sensitivity (limits of quantification = 15, 10, and 20 ng/mL for K, NK, and DHNK, respectively), accuracy (90-104%), and precision (RSD<8.1%) for all analytes. Two hundred and six urine specimens collected from suspected drug users were analyzed by this protocol and also screened by Neogen ELISA method to evaluate the efficiency as well as the compatibility of these two methods. Neogen ELISA showed high efficiency (98.1%), high sensitivity (90.9%), high specificity (98.9%), low false-positive rate (1.1%), and moderate false-negative rate (9.1%), adopting 10 ng/mL K as the cutoff. Neogen ELISA screening followed by GC-MS analysis appeared to be a good screening-confirmation test scheme for the analysis of K in urine. Twenty of the 22 positive urine specimens contained all three analytes simultaneously, with DHNK showing the highest and K the lowest concentrations. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17339137/GC_MS_quantification_of_ketamine_norketamine_and_dehydronorketamine_in_urine_specimens_and_comparative_study_using_ELISA_as_the_preliminary_test_methodology_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00102-X DB - PRIME DP - Unbound Medicine ER -