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Renal vascular and tubulointerstitial inflammation and proliferation in Cyp1a1-Ren2 transgenic rats with inducible ANG II-dependent malignant hypertension.
Am J Physiol Renal Physiol. 2007 Jun; 292(6):F1858-66.AJ

Abstract

Transgenic rats with inducible ANG II-dependent malignant hypertension [TGR(Cyp1a1Ren2)] were generated by inserting the mouse Ren2 renin gene into the genome of the rat. The present study was performed to assess renal morphological changes occurring during the development of ANG II-dependent malignant hypertension in these rats. Male Cyp1a1-Ren2 rats (n = 10) were fed normal rat food containing indole-3-carbinol (I3C; 0.3%) for 10 days to induce malignant hypertension. Rats induced with I3C had higher mean arterial pressures (173 +/- 9 vs. 112 +/- 11 mmHg, P < 0.01) than noninduced normotensive rats (n = 9). Glomerular damage was evaluated by determination of the glomerulosclerosis index (GSI) in tissue sections stained with periodic acid-Schiff. Kidneys of hypertensive rats had a higher GSI than normotensive rats (21.3 +/- 5.6 vs. 3.5 +/- 1.31 units). Quantitative analysis of macrophage ED-1-positive cells and proliferating cell nuclear antigen using immunohistochemistry demonstrated increased macrophage numbers in the renal interstitium (106.4 +/- 11.4 vs. 58.7 +/- 5.0 cells/mm(2)) and increased proliferating cell number in cortical tubules (37.8 +/- 5.7 vs. 24.2 +/- 2.1 cells/mm(2)), renal cortical vessels (2.2 +/- 0.5 vs. 0.13 +/- 0.07 cells/vessel), and the cortical interstitium (33.6 +/- 5.7 vs. 4.2 +/- 1.4 cells/mm(2)) of hypertensive rat kidneys. These findings demonstrate that the renal pathological changes that occur during the development of malignant hypertension in Cyp1a1-Ren2 rats are characterized by inflammation and cellular proliferation in cortical vessels and tubulointerstitium.

Authors+Show Affiliations

Department of Physiology, Hypertension and Renal Center of Excellence, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17344186

Citation

Graciano, Miguel L., et al. "Renal Vascular and Tubulointerstitial Inflammation and Proliferation in Cyp1a1-Ren2 Transgenic Rats With Inducible ANG II-dependent Malignant Hypertension." American Journal of Physiology. Renal Physiology, vol. 292, no. 6, 2007, pp. F1858-66.
Graciano ML, Mouton CR, Patterson ME, et al. Renal vascular and tubulointerstitial inflammation and proliferation in Cyp1a1-Ren2 transgenic rats with inducible ANG II-dependent malignant hypertension. Am J Physiol Renal Physiol. 2007;292(6):F1858-66.
Graciano, M. L., Mouton, C. R., Patterson, M. E., Seth, D. M., Mullins, J. J., & Mitchell, K. D. (2007). Renal vascular and tubulointerstitial inflammation and proliferation in Cyp1a1-Ren2 transgenic rats with inducible ANG II-dependent malignant hypertension. American Journal of Physiology. Renal Physiology, 292(6), F1858-66.
Graciano ML, et al. Renal Vascular and Tubulointerstitial Inflammation and Proliferation in Cyp1a1-Ren2 Transgenic Rats With Inducible ANG II-dependent Malignant Hypertension. Am J Physiol Renal Physiol. 2007;292(6):F1858-66. PubMed PMID: 17344186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Renal vascular and tubulointerstitial inflammation and proliferation in Cyp1a1-Ren2 transgenic rats with inducible ANG II-dependent malignant hypertension. AU - Graciano,Miguel L, AU - Mouton,Cynthia R, AU - Patterson,Matthew E, AU - Seth,Dale M, AU - Mullins,John J, AU - Mitchell,Kenneth D, Y1 - 2007/03/06/ PY - 2007/3/9/pubmed PY - 2007/7/26/medline PY - 2007/3/9/entrez SP - F1858 EP - 66 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 292 IS - 6 N2 - Transgenic rats with inducible ANG II-dependent malignant hypertension [TGR(Cyp1a1Ren2)] were generated by inserting the mouse Ren2 renin gene into the genome of the rat. The present study was performed to assess renal morphological changes occurring during the development of ANG II-dependent malignant hypertension in these rats. Male Cyp1a1-Ren2 rats (n = 10) were fed normal rat food containing indole-3-carbinol (I3C; 0.3%) for 10 days to induce malignant hypertension. Rats induced with I3C had higher mean arterial pressures (173 +/- 9 vs. 112 +/- 11 mmHg, P < 0.01) than noninduced normotensive rats (n = 9). Glomerular damage was evaluated by determination of the glomerulosclerosis index (GSI) in tissue sections stained with periodic acid-Schiff. Kidneys of hypertensive rats had a higher GSI than normotensive rats (21.3 +/- 5.6 vs. 3.5 +/- 1.31 units). Quantitative analysis of macrophage ED-1-positive cells and proliferating cell nuclear antigen using immunohistochemistry demonstrated increased macrophage numbers in the renal interstitium (106.4 +/- 11.4 vs. 58.7 +/- 5.0 cells/mm(2)) and increased proliferating cell number in cortical tubules (37.8 +/- 5.7 vs. 24.2 +/- 2.1 cells/mm(2)), renal cortical vessels (2.2 +/- 0.5 vs. 0.13 +/- 0.07 cells/vessel), and the cortical interstitium (33.6 +/- 5.7 vs. 4.2 +/- 1.4 cells/mm(2)) of hypertensive rat kidneys. These findings demonstrate that the renal pathological changes that occur during the development of malignant hypertension in Cyp1a1-Ren2 rats are characterized by inflammation and cellular proliferation in cortical vessels and tubulointerstitium. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/17344186/Renal_vascular_and_tubulointerstitial_inflammation_and_proliferation_in_Cyp1a1_Ren2_transgenic_rats_with_inducible_ANG_II_dependent_malignant_hypertension_ L2 - http://journals.physiology.org/doi/full/10.1152/ajprenal.00469.2006?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -