Tags

Type your tag names separated by a space and hit enter

Melatonin and its synthesizing enzymes (arylalkylamine N-acetyltransferase-like and hydroxyindole-O-methyltransferase) in avian eggs and early embryos.
J Pineal Res. 2007 Apr; 42(3):310-8.JP

Abstract

The presence of melatonin and the enzymes (transcripts and activities) involved in its synthesis, i.e. arylalkylamine N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT), was investigated in the eggs and early embryos of Japanese quail at Hamburger-Hamilton stages 1-10. Melatonin was present in the egg yolk (approximately 70 pg/g) and albumen (approximately 20 pg/g). The average content of melatonin was approximately 416 pg/egg. AA-NAT and HIOMT transcripts were present in the oocytes, blastoderms, and ovarian follicles. AA-NAT-like and HIOMT activities were detected in quail egg yolk. The activity of AA-NAT in yolk was comparable with that found in the pineal gland when calculated per milligram of yolk or pineal gland, but was significantly lower when re-calculated per milligram of protein in the yolk or pineal gland. AA-NAT-like activity was also identified in the ovarian follicles. Low HIOMT activity was detected in yolk, but not in the ovarian follicle. Both enzymes were essentially absent from early embryos although some residual activities, probably of yolk origin, were present in the stage 1 embryo. Melatonin and all the constituents needed for its synthesis (serotonin, AA-NAT and HIOMT activities) are contained within the avian yolk and could be used by the embryo from the very beginning of its development. The role of extrapineal melatonin in early avian development may be in protecting the embryo from the action of free radicals formed during intensive embryonic metabolism and/or it may participate (together with serotonin) in a 'diffuse neuroendocrine system' acting at early developmental stages, before differentiation of the nervous system.

Authors+Show Affiliations

Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec n/Warsaw, Poland. b.olszanska@ighz.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17349030

Citation

Olszańska, Bozenna, et al. "Melatonin and Its Synthesizing Enzymes (arylalkylamine N-acetyltransferase-like and hydroxyindole-O-methyltransferase) in Avian Eggs and Early Embryos." Journal of Pineal Research, vol. 42, no. 3, 2007, pp. 310-8.
Olszańska B, Bozenna O, Majewski P, et al. Melatonin and its synthesizing enzymes (arylalkylamine N-acetyltransferase-like and hydroxyindole-O-methyltransferase) in avian eggs and early embryos. J Pineal Res. 2007;42(3):310-8.
Olszańska, B., Bozenna, O., Majewski, P., Paweł, M., Lewczuk, B., Bogdan, L., Stepińska, U., & Urszula, S. (2007). Melatonin and its synthesizing enzymes (arylalkylamine N-acetyltransferase-like and hydroxyindole-O-methyltransferase) in avian eggs and early embryos. Journal of Pineal Research, 42(3), 310-8.
Olszańska B, et al. Melatonin and Its Synthesizing Enzymes (arylalkylamine N-acetyltransferase-like and hydroxyindole-O-methyltransferase) in Avian Eggs and Early Embryos. J Pineal Res. 2007;42(3):310-8. PubMed PMID: 17349030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melatonin and its synthesizing enzymes (arylalkylamine N-acetyltransferase-like and hydroxyindole-O-methyltransferase) in avian eggs and early embryos. AU - Olszańska,Bozenna, AU - Bozenna,Olszańska, AU - Majewski,Paweł, AU - Paweł,Majewski, AU - Lewczuk,Bogdan, AU - Bogdan,Lewczuk, AU - Stepińska,Urszula, AU - Urszula,Stepińska, PY - 2007/3/14/pubmed PY - 2007/6/15/medline PY - 2007/3/14/entrez SP - 310 EP - 8 JF - Journal of pineal research JO - J Pineal Res VL - 42 IS - 3 N2 - The presence of melatonin and the enzymes (transcripts and activities) involved in its synthesis, i.e. arylalkylamine N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT), was investigated in the eggs and early embryos of Japanese quail at Hamburger-Hamilton stages 1-10. Melatonin was present in the egg yolk (approximately 70 pg/g) and albumen (approximately 20 pg/g). The average content of melatonin was approximately 416 pg/egg. AA-NAT and HIOMT transcripts were present in the oocytes, blastoderms, and ovarian follicles. AA-NAT-like and HIOMT activities were detected in quail egg yolk. The activity of AA-NAT in yolk was comparable with that found in the pineal gland when calculated per milligram of yolk or pineal gland, but was significantly lower when re-calculated per milligram of protein in the yolk or pineal gland. AA-NAT-like activity was also identified in the ovarian follicles. Low HIOMT activity was detected in yolk, but not in the ovarian follicle. Both enzymes were essentially absent from early embryos although some residual activities, probably of yolk origin, were present in the stage 1 embryo. Melatonin and all the constituents needed for its synthesis (serotonin, AA-NAT and HIOMT activities) are contained within the avian yolk and could be used by the embryo from the very beginning of its development. The role of extrapineal melatonin in early avian development may be in protecting the embryo from the action of free radicals formed during intensive embryonic metabolism and/or it may participate (together with serotonin) in a 'diffuse neuroendocrine system' acting at early developmental stages, before differentiation of the nervous system. SN - 0742-3098 UR - https://www.unboundmedicine.com/medline/citation/17349030/Melatonin_and_its_synthesizing_enzymes__arylalkylamine_N_acetyltransferase_like_and_hydroxyindole_O_methyltransferase__in_avian_eggs_and_early_embryos_ L2 - https://doi.org/10.1111/j.1600-079X.2007.00421.x DB - PRIME DP - Unbound Medicine ER -