TY - JOUR T1 - Relationship between MPA free fraction and free MPAG concentrations in heart transplant recipients based on simultaneous HPLC quantification of the target compounds in human plasma. AU - Cussonneau,Xavier, AU - Bolon-Larger,Magali, AU - Prunet-Spano,Céline, AU - Bastien,Olivier, AU - Boulieu,Roselyne, Y1 - 2007/02/28/ PY - 2006/12/14/received PY - 2007/02/08/revised PY - 2007/02/16/accepted PY - 2007/3/14/pubmed PY - 2007/8/2/medline PY - 2007/3/14/entrez SP - 674 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 852 IS - 1-2 N2 - A simple and sensitive HPLC method for the simultaneous analysis of free MPA and free MPAG was developed. Separation was achieved on a X-Terra RP18 column with acetonitrile-40 mM orthophosphoric acid as eluents using a gradient elution mode over 35 min at a flow rate of 1.5 ml/min. The assay was linear in the range 0.005 mg/L (LOQ) to 5mg/L for free MPA and 0.05 mg/L (LOQ) to 200 mg/L for free MPAG. Isolation of free MPA and free MPAG was done by ultrafiltration and the ultrafiltrate was directly injected. A positive correlation between MPA free fractions and free MPAG concentrations was found. Likewise, free MPAG was related to total MPAG concentrations in the seven heart transplant patients. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/17350903/Relationship_between_MPA_free_fraction_and_free_MPAG_concentrations_in_heart_transplant_recipients_based_on_simultaneous_HPLC_quantification_of_the_target_compounds_in_human_plasma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(07)00162-6 DB - PRIME DP - Unbound Medicine ER -