Tags

Type your tag names separated by a space and hit enter

Nano-liquid chromatography analysis of dansylated biogenic amines in wines.
J Chromatogr A. 2007 Apr 20; 1147(2):192-9.JC

Abstract

In this work, the simultaneous analysis of 10 biogenic amines (ethanolamine, methylamine, tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine) in wines by nano-liquid chromatography (nano-LC) using UV detection and a capillary bidentate C(18) column of 100 microm I.D. is proposed. The 10 selected amines, which are the most important to be determined in wine samples, were derivatized with dansyl-chloride (Dns-Cl) previous to their nano-LC determination. Excess of the derivatizing agent as well as other components of the samples were eliminated by the use of an on-line cleaning step employing a C(18) trapping column which also provided a pre-concentration effect. The mobile phase composed of acetonitrile, water, acetic acid and triethylamine (TEA) mixture was pumped at a low flow rate (634 nL/min). Limits of detection (LODs) achieved ranged between 18.3 and 48.3 ng/mL; while calibration curves showed good linearity (R(2)>0.9924). The method was applied to the analysis of this group of amines in white and red wine samples after suitable treatment with polyvinylpyrrolidone (PVP) and extraction with C(18) cartridges.

Authors+Show Affiliations

Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, P.O. Box 10, Area della Ricerca di Roma, Monterotondo Scalo, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17353021

Citation

Hernández-Borges, Javier, et al. "Nano-liquid Chromatography Analysis of Dansylated Biogenic Amines in Wines." Journal of Chromatography. A, vol. 1147, no. 2, 2007, pp. 192-9.
Hernández-Borges J, D'Orazio G, Aturki Z, et al. Nano-liquid chromatography analysis of dansylated biogenic amines in wines. J Chromatogr A. 2007;1147(2):192-9.
Hernández-Borges, J., D'Orazio, G., Aturki, Z., & Fanali, S. (2007). Nano-liquid chromatography analysis of dansylated biogenic amines in wines. Journal of Chromatography. A, 1147(2), 192-9.
Hernández-Borges J, et al. Nano-liquid Chromatography Analysis of Dansylated Biogenic Amines in Wines. J Chromatogr A. 2007 Apr 20;1147(2):192-9. PubMed PMID: 17353021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nano-liquid chromatography analysis of dansylated biogenic amines in wines. AU - Hernández-Borges,Javier, AU - D'Orazio,Giovanni, AU - Aturki,Zeineb, AU - Fanali,Salvatore, Y1 - 2007/02/24/ PY - 2006/12/12/received PY - 2007/02/13/revised PY - 2007/02/19/accepted PY - 2007/3/14/pubmed PY - 2007/6/1/medline PY - 2007/3/14/entrez SP - 192 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1147 IS - 2 N2 - In this work, the simultaneous analysis of 10 biogenic amines (ethanolamine, methylamine, tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine) in wines by nano-liquid chromatography (nano-LC) using UV detection and a capillary bidentate C(18) column of 100 microm I.D. is proposed. The 10 selected amines, which are the most important to be determined in wine samples, were derivatized with dansyl-chloride (Dns-Cl) previous to their nano-LC determination. Excess of the derivatizing agent as well as other components of the samples were eliminated by the use of an on-line cleaning step employing a C(18) trapping column which also provided a pre-concentration effect. The mobile phase composed of acetonitrile, water, acetic acid and triethylamine (TEA) mixture was pumped at a low flow rate (634 nL/min). Limits of detection (LODs) achieved ranged between 18.3 and 48.3 ng/mL; while calibration curves showed good linearity (R(2)>0.9924). The method was applied to the analysis of this group of amines in white and red wine samples after suitable treatment with polyvinylpyrrolidone (PVP) and extraction with C(18) cartridges. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17353021/Nano_liquid_chromatography_analysis_of_dansylated_biogenic_amines_in_wines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00364-0 DB - PRIME DP - Unbound Medicine ER -