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In vitro activity of an ear rinse containing tromethamine, EDTA, benzyl alcohol and 0.1% ketoconazole on Malassezia organisms from dogs with otitis externa.
Vet Dermatol. 2007 Apr; 18(2):115-9.VD

Abstract

The purpose of this study was to evaluate the in vitro activity of an ear rinse containing tromethamine, EDTA, benzyl alcohol and 0.1% ketoconazole in purified water on Malassezia organisms from dogs with otitis externa. Malassezia organisms were collected from ear swab samples from the external ear canal of 19 dogs with otitis externa plus one control strain of Malassezia pachydermatis. Three test solutions were evaluated: ER (EDTA, tromethamine, benzyl alcohol), ER + keto (EDTA, tromethamine, benzyl alcohol, ketoconazole), and H2O (purified water). Ten-millilitre aliquots of each test solution was transferred into 20 tubes and inoculated with one of the isolates (1 tube per isolate: 19 clinical and 1 control strain). Samples were retrieved from each tube at five time points (0, 15, 30, 45 and 60 min), transferred to Petri dishes, mixed with Sabouraud dextrose agar supplemented with 0.5% Tween 80 and incubated. Following incubation, the plates were examined for growth and colonies counted as colony-forming units per millilitre. The data were analysed using a repeated measures analysis, with pair-wise comparisons of solution-time combinations. There was a significant reduction in Malassezia growth in ER + keto at all time points (P < 0.0001) compared to time zero. Neither ER nor H2O had any effect on the growth of Malassezia. ER + keto was significantly more effective in reducing Malassezia growth (P < 0.0001) at all time points compared to both ER and H2O. ER + keto may be useful in the treatment of Malassezia otitis externa. Future studies should be performed to evaluate the in vivo efficacy of ER + keto as treatment for otic infections caused by Malassezia.

Authors+Show Affiliations

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA. cole.143@osu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17355426

Citation

Cole, Lynette K., et al. "In Vitro Activity of an Ear Rinse Containing Tromethamine, EDTA, Benzyl Alcohol and 0.1% Ketoconazole On Malassezia Organisms From Dogs With Otitis Externa." Veterinary Dermatology, vol. 18, no. 2, 2007, pp. 115-9.
Cole LK, Luu DH, Rajala-Schultz PJ, et al. In vitro activity of an ear rinse containing tromethamine, EDTA, benzyl alcohol and 0.1% ketoconazole on Malassezia organisms from dogs with otitis externa. Vet Dermatol. 2007;18(2):115-9.
Cole, L. K., Luu, D. H., Rajala-Schultz, P. J., Meadows, C., & Torres, A. H. (2007). In vitro activity of an ear rinse containing tromethamine, EDTA, benzyl alcohol and 0.1% ketoconazole on Malassezia organisms from dogs with otitis externa. Veterinary Dermatology, 18(2), 115-9.
Cole LK, et al. In Vitro Activity of an Ear Rinse Containing Tromethamine, EDTA, Benzyl Alcohol and 0.1% Ketoconazole On Malassezia Organisms From Dogs With Otitis Externa. Vet Dermatol. 2007;18(2):115-9. PubMed PMID: 17355426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro activity of an ear rinse containing tromethamine, EDTA, benzyl alcohol and 0.1% ketoconazole on Malassezia organisms from dogs with otitis externa. AU - Cole,Lynette K, AU - Luu,Dao H, AU - Rajala-Schultz,Paivi J, AU - Meadows,Cheyney, AU - Torres,Audrey H, PY - 2007/3/16/pubmed PY - 2007/5/16/medline PY - 2007/3/16/entrez SP - 115 EP - 9 JF - Veterinary dermatology JO - Vet. Dermatol. VL - 18 IS - 2 N2 - The purpose of this study was to evaluate the in vitro activity of an ear rinse containing tromethamine, EDTA, benzyl alcohol and 0.1% ketoconazole in purified water on Malassezia organisms from dogs with otitis externa. Malassezia organisms were collected from ear swab samples from the external ear canal of 19 dogs with otitis externa plus one control strain of Malassezia pachydermatis. Three test solutions were evaluated: ER (EDTA, tromethamine, benzyl alcohol), ER + keto (EDTA, tromethamine, benzyl alcohol, ketoconazole), and H2O (purified water). Ten-millilitre aliquots of each test solution was transferred into 20 tubes and inoculated with one of the isolates (1 tube per isolate: 19 clinical and 1 control strain). Samples were retrieved from each tube at five time points (0, 15, 30, 45 and 60 min), transferred to Petri dishes, mixed with Sabouraud dextrose agar supplemented with 0.5% Tween 80 and incubated. Following incubation, the plates were examined for growth and colonies counted as colony-forming units per millilitre. The data were analysed using a repeated measures analysis, with pair-wise comparisons of solution-time combinations. There was a significant reduction in Malassezia growth in ER + keto at all time points (P < 0.0001) compared to time zero. Neither ER nor H2O had any effect on the growth of Malassezia. ER + keto was significantly more effective in reducing Malassezia growth (P < 0.0001) at all time points compared to both ER and H2O. ER + keto may be useful in the treatment of Malassezia otitis externa. Future studies should be performed to evaluate the in vivo efficacy of ER + keto as treatment for otic infections caused by Malassezia. SN - 0959-4493 UR - https://www.unboundmedicine.com/medline/citation/17355426/In_vitro_activity_of_an_ear_rinse_containing_tromethamine_EDTA_benzyl_alcohol_and_0_1_ketoconazole_on_Malassezia_organisms_from_dogs_with_otitis_externa_ L2 - https://doi.org/10.1111/j.1365-3164.2007.00583.x DB - PRIME DP - Unbound Medicine ER -