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Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat.
Theor Appl Genet. 2007 May; 114(8):1379-89.TA

Abstract

Leaf rust and stripe rust are important foliar diseases of wheat worldwide. Leaf rust and stripe rust resistant introgression lines were developed by induced homoeologous chromosome pairing between wheat chromosome 5D and 5M(g) of Aegilops geniculata (U(g)M(g)). Characterization of rust resistant BC(2)F(5) and BC(3)F(6) homozygous progenies using genomic in situ hybridization with Aegilops comosa (M) DNA as probe identified three different types of introgressions; two cytologically visible and one invisible (termed cryptic alien introgression). All three types of introgression lines showed similar and complete resistance to the most prevalent pathotypes of leaf rust and stripe rust in Kansas (USA) and Punjab (India). Diagnostic polymorphisms between the alien segment and recipient parent were identified using physically mapped RFLP probes. Molecular mapping revealed that cryptic alien introgression conferring resistance to leaf rust and stripe rust comprised less than 5% of the 5DS arm and was designated T5DL.5DS-5M(g)S(0.95). Genetic mapping with an F(2)population of Wichita x T5DL.5DS-5M(g)S(0.95) demonstrated the monogenic and dominant inheritance of resistance to both diseases. Two diagnostic RFLP markers, previously mapped on chromosome arm 5DS, co-segregated with the rust resistance in the F(2) population. The unique map location of the resistant introgression on chromosome T5DL.5DS-5M(g)S(0.95) suggested that the leaf rust and stripe rust resistance genes were new and were designated Lr57 and Yr40. This is the first documentation of a successful transfer and characterization of cryptic alien introgression from Ae. geniculata conferring resistance to both leaf rust and stripe rust in wheat.

Authors+Show Affiliations

Kuraparthy V
Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA.
Chhuneja P
No affiliation info available
Dhaliwal HS
No affiliation info available
Kaur S
No affiliation info available
Bowden RL
No affiliation info available
Gill BS
No affiliation info available

MeSH

BasidiomycotaChromosome MappingImmunity, InnatePlant DiseasesPlant ProteinsPoaceaeTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17356867

Citation

Kuraparthy, Vasu, et al. "Characterization and Mapping of Cryptic Alien Introgression From Aegilops Geniculata With New Leaf Rust and Stripe Rust Resistance Genes Lr57 and Yr40 in Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 114, no. 8, 2007, pp. 1379-89.
Kuraparthy V, Chhuneja P, Dhaliwal HS, et al. Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theor Appl Genet. 2007;114(8):1379-89.
Kuraparthy, V., Chhuneja, P., Dhaliwal, H. S., Kaur, S., Bowden, R. L., & Gill, B. S. (2007). Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 114(8), 1379-89.
Kuraparthy V, et al. Characterization and Mapping of Cryptic Alien Introgression From Aegilops Geniculata With New Leaf Rust and Stripe Rust Resistance Genes Lr57 and Yr40 in Wheat. Theor Appl Genet. 2007;114(8):1379-89. PubMed PMID: 17356867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. AU - Kuraparthy,Vasu, AU - Chhuneja,Parveen, AU - Dhaliwal,Harcharan S, AU - Kaur,Satinder, AU - Bowden,Robert L, AU - Gill,Bikram S, Y1 - 2007/03/14/ PY - 2006/10/18/received PY - 2007/02/05/accepted PY - 2007/3/16/pubmed PY - 2007/8/19/medline PY - 2007/3/16/entrez SP - 1379 EP - 89 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 114 IS - 8 N2 - Leaf rust and stripe rust are important foliar diseases of wheat worldwide. Leaf rust and stripe rust resistant introgression lines were developed by induced homoeologous chromosome pairing between wheat chromosome 5D and 5M(g) of Aegilops geniculata (U(g)M(g)). Characterization of rust resistant BC(2)F(5) and BC(3)F(6) homozygous progenies using genomic in situ hybridization with Aegilops comosa (M) DNA as probe identified three different types of introgressions; two cytologically visible and one invisible (termed cryptic alien introgression). All three types of introgression lines showed similar and complete resistance to the most prevalent pathotypes of leaf rust and stripe rust in Kansas (USA) and Punjab (India). Diagnostic polymorphisms between the alien segment and recipient parent were identified using physically mapped RFLP probes. Molecular mapping revealed that cryptic alien introgression conferring resistance to leaf rust and stripe rust comprised less than 5% of the 5DS arm and was designated T5DL.5DS-5M(g)S(0.95). Genetic mapping with an F(2)population of Wichita x T5DL.5DS-5M(g)S(0.95) demonstrated the monogenic and dominant inheritance of resistance to both diseases. Two diagnostic RFLP markers, previously mapped on chromosome arm 5DS, co-segregated with the rust resistance in the F(2) population. The unique map location of the resistant introgression on chromosome T5DL.5DS-5M(g)S(0.95) suggested that the leaf rust and stripe rust resistance genes were new and were designated Lr57 and Yr40. This is the first documentation of a successful transfer and characterization of cryptic alien introgression from Ae. geniculata conferring resistance to both leaf rust and stripe rust in wheat. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/17356867/Characterization_and_mapping_of_cryptic_alien_introgression_from_Aegilops_geniculata_with_new_leaf_rust_and_stripe_rust_resistance_genes_Lr57_and_Yr40_in_wheat_ DB - PRIME DP - Unbound Medicine ER -