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Crystal structure of an archaeal homologue of multidrug resistance repressor protein, EmrR, from hyperthermophilic archaea Sulfolobus tokodaii strain 7.
Proteins. 2007 Jun 01; 67(4):1138-46.P

Abstract

MarR family proteins, MarR, MexR, and EmrR, are known as bacterial regulators for a phenotype resistant to multiple antibiotic drugs. Genomic data have indicated the presence of bacterial-type transcriptional regulators, including MarR family proteins in archaea, though the archaeal transcription system is close to that of eukaryote. To elucidate the structural basis of the transcriptional regulation mechanism of archaeal MarR family proteins, the crystal structure of the ST1710 protein, which was identified as an archaeal EmrR homologue, StEmrR, from hyperthermophilic archaeon Sulfolobus tokodaii strain 7 was determined at 1.45-A resolution. The protein was composed of two N- and C-terminal dimerization domains, and the DNA-binding domain consisted of a winged helix motif, as in the case of bacterial MarR family proteins. Despite the relatively low overall structural similarity between StEmrR and bacterial MarR family proteins, the structure of the DNA-binding domain displayed high structural similarity. A comparison with the crystal structures of bacterial MarR family proteins revealed that structural variation was mainly due to the different orientation of the two helices at the N- and C-termini. Our results indicated that the distance between the two DNA-binding domains of MarR family proteins would be changed by the rotation of the two terminal helices to interact with the target DNA.

Authors+Show Affiliations

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17357153

Citation

Miyazono, Ken-ichi, et al. "Crystal Structure of an Archaeal Homologue of Multidrug Resistance Repressor Protein, EmrR, From Hyperthermophilic Archaea Sulfolobus Tokodaii Strain 7." Proteins, vol. 67, no. 4, 2007, pp. 1138-46.
Miyazono K, Tsujimura M, Kawarabayasi Y, et al. Crystal structure of an archaeal homologue of multidrug resistance repressor protein, EmrR, from hyperthermophilic archaea Sulfolobus tokodaii strain 7. Proteins. 2007;67(4):1138-46.
Miyazono, K., Tsujimura, M., Kawarabayasi, Y., & Tanokura, M. (2007). Crystal structure of an archaeal homologue of multidrug resistance repressor protein, EmrR, from hyperthermophilic archaea Sulfolobus tokodaii strain 7. Proteins, 67(4), 1138-46.
Miyazono K, et al. Crystal Structure of an Archaeal Homologue of Multidrug Resistance Repressor Protein, EmrR, From Hyperthermophilic Archaea Sulfolobus Tokodaii Strain 7. Proteins. 2007 Jun 1;67(4):1138-46. PubMed PMID: 17357153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystal structure of an archaeal homologue of multidrug resistance repressor protein, EmrR, from hyperthermophilic archaea Sulfolobus tokodaii strain 7. AU - Miyazono,Ken-ichi, AU - Tsujimura,Masanari, AU - Kawarabayasi,Yutaka, AU - Tanokura,Masaru, PY - 2007/3/16/pubmed PY - 2007/6/8/medline PY - 2007/3/16/entrez SP - 1138 EP - 46 JF - Proteins JO - Proteins VL - 67 IS - 4 N2 - MarR family proteins, MarR, MexR, and EmrR, are known as bacterial regulators for a phenotype resistant to multiple antibiotic drugs. Genomic data have indicated the presence of bacterial-type transcriptional regulators, including MarR family proteins in archaea, though the archaeal transcription system is close to that of eukaryote. To elucidate the structural basis of the transcriptional regulation mechanism of archaeal MarR family proteins, the crystal structure of the ST1710 protein, which was identified as an archaeal EmrR homologue, StEmrR, from hyperthermophilic archaeon Sulfolobus tokodaii strain 7 was determined at 1.45-A resolution. The protein was composed of two N- and C-terminal dimerization domains, and the DNA-binding domain consisted of a winged helix motif, as in the case of bacterial MarR family proteins. Despite the relatively low overall structural similarity between StEmrR and bacterial MarR family proteins, the structure of the DNA-binding domain displayed high structural similarity. A comparison with the crystal structures of bacterial MarR family proteins revealed that structural variation was mainly due to the different orientation of the two helices at the N- and C-termini. Our results indicated that the distance between the two DNA-binding domains of MarR family proteins would be changed by the rotation of the two terminal helices to interact with the target DNA. SN - 1097-0134 UR - https://www.unboundmedicine.com/medline/citation/17357153/Crystal_structure_of_an_archaeal_homologue_of_multidrug_resistance_repressor_protein_EmrR_from_hyperthermophilic_archaea_Sulfolobus_tokodaii_strain_7_ L2 - https://doi.org/10.1002/prot.21327 DB - PRIME DP - Unbound Medicine ER -