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Antimutagenic effect of broccoli flower head by the ames salmonella reverse mutation assay.
Phytother Res 2007; 21(6):545-7PR

Abstract

A study was performed to investigate the antimutagenic effect of broccoli flower head by the Ames Salmonella reverse mutation assay. Broccoli flower head being the most highly edible part in the plant was analysed for its antimutagenic effect. Without isolating the phytomolecules, the crude ethanol extract of broccoli flower head was tested for suppressing the mutagenic effect induced by certain chemical mutagens. Three strains - TA 98, TA102 and TA 1535 were used in the study. The tester strains were challenged with their respective mutagens. These were challenged with the ethanol extract of broccoli flower head at concentrations of 23 and 46 mg/plate. The plates were incubated for 72 h and the revertant colonies were counted. The crude extract did not prove to be promutagenic. The ethanol extract of the broccoli flower head at 46 mg/plate suppressed the mutagenic effect induced by the corresponding positive mutagens on all the three tester strains used in this study. The crude extract of broccoli flower head alone was not cytotoxic even at the maximum concentration tested (46 mg/plate). In conclusion, the ethanol extract of broccoli at 46 mg/plate suggests their diverse antimutagenic potential against the mutagenic chemicals employed in this study.

Authors+Show Affiliations

Department of Genetic Toxicology, International Institute of Biotechnology and Toxicology (IIBAT), Padappai-601301, Kancheepuram District, Tamil Nadu, India. drssmurugan@hotmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17357172

Citation

Murugan, S S., et al. "Antimutagenic Effect of Broccoli Flower Head By the Ames Salmonella Reverse Mutation Assay." Phytotherapy Research : PTR, vol. 21, no. 6, 2007, pp. 545-7.
Murugan SS, Balakrishnamurthy P, Mathew YJ. Antimutagenic effect of broccoli flower head by the ames salmonella reverse mutation assay. Phytother Res. 2007;21(6):545-7.
Murugan, S. S., Balakrishnamurthy, P., & Mathew, Y. J. (2007). Antimutagenic effect of broccoli flower head by the ames salmonella reverse mutation assay. Phytotherapy Research : PTR, 21(6), pp. 545-7.
Murugan SS, Balakrishnamurthy P, Mathew YJ. Antimutagenic Effect of Broccoli Flower Head By the Ames Salmonella Reverse Mutation Assay. Phytother Res. 2007;21(6):545-7. PubMed PMID: 17357172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimutagenic effect of broccoli flower head by the ames salmonella reverse mutation assay. AU - Murugan,S S, AU - Balakrishnamurthy,P, AU - Mathew,Y J, PY - 2007/3/16/pubmed PY - 2007/9/18/medline PY - 2007/3/16/entrez SP - 545 EP - 7 JF - Phytotherapy research : PTR JO - Phytother Res VL - 21 IS - 6 N2 - A study was performed to investigate the antimutagenic effect of broccoli flower head by the Ames Salmonella reverse mutation assay. Broccoli flower head being the most highly edible part in the plant was analysed for its antimutagenic effect. Without isolating the phytomolecules, the crude ethanol extract of broccoli flower head was tested for suppressing the mutagenic effect induced by certain chemical mutagens. Three strains - TA 98, TA102 and TA 1535 were used in the study. The tester strains were challenged with their respective mutagens. These were challenged with the ethanol extract of broccoli flower head at concentrations of 23 and 46 mg/plate. The plates were incubated for 72 h and the revertant colonies were counted. The crude extract did not prove to be promutagenic. The ethanol extract of the broccoli flower head at 46 mg/plate suppressed the mutagenic effect induced by the corresponding positive mutagens on all the three tester strains used in this study. The crude extract of broccoli flower head alone was not cytotoxic even at the maximum concentration tested (46 mg/plate). In conclusion, the ethanol extract of broccoli at 46 mg/plate suggests their diverse antimutagenic potential against the mutagenic chemicals employed in this study. SN - 0951-418X UR - https://www.unboundmedicine.com/medline/citation/17357172/Antimutagenic_effect_of_broccoli_flower_head_by_the_ames_salmonella_reverse_mutation_assay_ L2 - https://doi.org/10.1002/ptr.2104 DB - PRIME DP - Unbound Medicine ER -