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Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei.
Zoonoses Public Health. 2007; 54(1):44-50.ZP

Abstract

A PCR assay targeting the metalloprotease gene (mprA) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei.

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Authors+Show Affiliations

Neubauer H
Friedrich-Loeffler-Institut, Naumburger Str. 96a, D-07743 Jena, Germany. heinrich.neubauer@fli.bund.de
Sprague LD
No affiliation info available
Joseph M
No affiliation info available
Tomaso H
No affiliation info available
Al Dahouk S
No affiliation info available
Witte A
No affiliation info available
Kinne J
No affiliation info available
Hensel A
No affiliation info available
Wernery R
No affiliation info available
Wernery U
No affiliation info available
Scholz HC
No affiliation info available

MeSH

AnimalsBase SequenceBurkholderia pseudomalleiDNA PrimersDNA, BacterialGene AmplificationHumansMetalloproteasesMolecular Sequence DataPolymerase Chain ReactionSensitivity and SpecificitySpecies Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17359446

Citation

Neubauer, H, et al. "Development and Clinical Evaluation of a PCR Assay Targeting the Metalloprotease Gene (mprA) of B. Pseudomallei." Zoonoses and Public Health, vol. 54, no. 1, 2007, pp. 44-50.
Neubauer H, Sprague LD, Joseph M, et al. Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei. Zoonoses Public Health. 2007;54(1):44-50.
Neubauer, H., Sprague, L. D., Joseph, M., Tomaso, H., Al Dahouk, S., Witte, A., Kinne, J., Hensel, A., Wernery, R., Wernery, U., & Scholz, H. C. (2007). Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei. Zoonoses and Public Health, 54(1), 44-50.
Neubauer H, et al. Development and Clinical Evaluation of a PCR Assay Targeting the Metalloprotease Gene (mprA) of B. Pseudomallei. Zoonoses Public Health. 2007;54(1):44-50. PubMed PMID: 17359446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei. AU - Neubauer,H, AU - Sprague,L D, AU - Joseph,M, AU - Tomaso,H, AU - Al Dahouk,S, AU - Witte,A, AU - Kinne,J, AU - Hensel,A, AU - Wernery,R, AU - Wernery,U, AU - Scholz,H C, PY - 2007/3/16/pubmed PY - 2007/4/6/medline PY - 2007/3/16/entrez SP - 44 EP - 50 JF - Zoonoses and public health JO - Zoonoses Public Health VL - 54 IS - 1 N2 - A PCR assay targeting the metalloprotease gene (mprA) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei. SN - 1863-1959 UR - https://www.unboundmedicine.com/medline/citation/17359446/Development_and_clinical_evaluation_of_a_PCR_assay_targeting_the_metalloprotease_gene__mprA__of_B__pseudomallei_ L2 - https://doi.org/10.1111/j.1863-2378.2007.01008.x DB - PRIME DP - Unbound Medicine ER -