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Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei.
Zoonoses Public Health. 2007; 54(1):44-50.ZP

Abstract

A PCR assay targeting the metalloprotease gene (mprA) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei.

Authors+Show Affiliations

Friedrich-Loeffler-Institut, Naumburger Str. 96a, D-07743 Jena, Germany. heinrich.neubauer@fli.bund.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17359446

Citation

Neubauer, H, et al. "Development and Clinical Evaluation of a PCR Assay Targeting the Metalloprotease Gene (mprA) of B. Pseudomallei." Zoonoses and Public Health, vol. 54, no. 1, 2007, pp. 44-50.
Neubauer H, Sprague LD, Joseph M, et al. Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei. Zoonoses Public Health. 2007;54(1):44-50.
Neubauer, H., Sprague, L. D., Joseph, M., Tomaso, H., Al Dahouk, S., Witte, A., Kinne, J., Hensel, A., Wernery, R., Wernery, U., & Scholz, H. C. (2007). Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei. Zoonoses and Public Health, 54(1), 44-50.
Neubauer H, et al. Development and Clinical Evaluation of a PCR Assay Targeting the Metalloprotease Gene (mprA) of B. Pseudomallei. Zoonoses Public Health. 2007;54(1):44-50. PubMed PMID: 17359446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei. AU - Neubauer,H, AU - Sprague,L D, AU - Joseph,M, AU - Tomaso,H, AU - Al Dahouk,S, AU - Witte,A, AU - Kinne,J, AU - Hensel,A, AU - Wernery,R, AU - Wernery,U, AU - Scholz,H C, PY - 2007/3/16/pubmed PY - 2007/4/6/medline PY - 2007/3/16/entrez SP - 44 EP - 50 JF - Zoonoses and public health JO - Zoonoses Public Health VL - 54 IS - 1 N2 - A PCR assay targeting the metalloprotease gene (mprA) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei. SN - 1863-1959 UR - https://www.unboundmedicine.com/medline/citation/17359446/Development_and_clinical_evaluation_of_a_PCR_assay_targeting_the_metalloprotease_gene__mprA__of_B__pseudomallei_ L2 - https://doi.org/10.1111/j.1863-2378.2007.01008.x DB - PRIME DP - Unbound Medicine ER -