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Infectivity studies of hepatitis A and B in non-human primates.
Dev Biol Stand 1975; 30:229-35DB

Abstract

We have induced hepatitis A in marmosets of the subspecies Saguinus mystax following primary inoculation with human serum containing the MS-1 strain of hepatitis A virus (HAV) and in 3 further marmoset subpassages using infective marmoset serum from each preceding passage. In each passage acquisition of serum antibody against 17 nm virus-like particles recovered from acute illness stools of human volunteers who developed hepatitis following inoculation with the MS-1 strain of HAV, as well as from acute illness stools of hepatitis A cases from a common source epidemic of heaptitis A in Arizona could be demonstrated by immune electron microscopy (IEM). Particle-containing stool filtrates from the latter epidemic also induced hepatitis in chimpanzees after intravenous inoculation. Inoculation of partially purified particles from a single banding in CsCl2 has further resulted in the induction of hepatitis in S. mystax marmosets. We have successfully induced hepatitis in a series of chimpanzees inoculated with sera containing hepatitis B surface antigen (HBsAg) of varying subspecificities. Susceptibility appears universal in animals who are initially lacking in serum antibody (anti-HBs) and antibody induced following experimental infection is homologous in subspecificity to the subspecificities of the antigenic coat components of the HBsAg in the inoculum. Results of cross challenge experiments indicate that animals developing hepatitis following inoculation of HBsAg of one subspecificity set in the d/y-w/r system do not again develop hepatitis following inoculation with HBsAg of the alternate subspecificity set.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

173599

Citation

Maynard, J E., et al. "Infectivity Studies of Hepatitis a and B in Non-human Primates." Developments in Biological Standardization, vol. 30, 1975, pp. 229-35.
Maynard JE, Krushak DH, Bradley DW, et al. Infectivity studies of hepatitis A and B in non-human primates. Dev Biol Stand. 1975;30:229-35.
Maynard, J. E., Krushak, D. H., Bradley, D. W., & Berquist, K. R. (1975). Infectivity studies of hepatitis A and B in non-human primates. Developments in Biological Standardization, 30, pp. 229-35.
Maynard JE, et al. Infectivity Studies of Hepatitis a and B in Non-human Primates. Dev Biol Stand. 1975;30:229-35. PubMed PMID: 173599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Infectivity studies of hepatitis A and B in non-human primates. AU - Maynard,J E, AU - Krushak,D H, AU - Bradley,D W, AU - Berquist,K R, PY - 1975/1/1/pubmed PY - 1975/1/1/medline PY - 1975/1/1/entrez SP - 229 EP - 35 JF - Developments in biological standardization JO - Dev. Biol. Stand. VL - 30 N2 - We have induced hepatitis A in marmosets of the subspecies Saguinus mystax following primary inoculation with human serum containing the MS-1 strain of hepatitis A virus (HAV) and in 3 further marmoset subpassages using infective marmoset serum from each preceding passage. In each passage acquisition of serum antibody against 17 nm virus-like particles recovered from acute illness stools of human volunteers who developed hepatitis following inoculation with the MS-1 strain of HAV, as well as from acute illness stools of hepatitis A cases from a common source epidemic of heaptitis A in Arizona could be demonstrated by immune electron microscopy (IEM). Particle-containing stool filtrates from the latter epidemic also induced hepatitis in chimpanzees after intravenous inoculation. Inoculation of partially purified particles from a single banding in CsCl2 has further resulted in the induction of hepatitis in S. mystax marmosets. We have successfully induced hepatitis in a series of chimpanzees inoculated with sera containing hepatitis B surface antigen (HBsAg) of varying subspecificities. Susceptibility appears universal in animals who are initially lacking in serum antibody (anti-HBs) and antibody induced following experimental infection is homologous in subspecificity to the subspecificities of the antigenic coat components of the HBsAg in the inoculum. Results of cross challenge experiments indicate that animals developing hepatitis following inoculation of HBsAg of one subspecificity set in the d/y-w/r system do not again develop hepatitis following inoculation with HBsAg of the alternate subspecificity set. SN - 0301-5149 UR - https://www.unboundmedicine.com/medline/citation/173599/Infectivity_studies_of_hepatitis_A_and_B_in_non_human_primates_ L2 - http://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -